Experiment: Bas14
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hemC and hemD overlap by 4 nucleotides hemD and hemX are separated by 21 nucleotides hemX and hemY are separated by 2 nucleotides
NIAGMN_12205: hemC - hydroxymethylbilane synthase, at 2,417,279 to 2,418,241
hemC
NIAGMN_12210: hemD - uroporphyrinogen-III synthase, at 2,418,238 to 2,418,978
hemD
NIAGMN_12215: hemX - uroporphyrinogen-III C-methyltransferase, at 2,419,000 to 2,420,205
hemX
NIAGMN_12220: hemY - protoheme IX biogenesis protein HemY, at 2,420,208 to 2,421,404
hemY
Position (kb)
2418
2419
2420
2421 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 2418.218 kb on + strand at 2418.218 kb on + strand at 2418.218 kb on + strand at 2418.218 kb on + strand at 2418.962 kb on + strand at 2418.971 kb on + strand at 2418.971 kb on + strand at 2418.971 kb on + strand at 2418.971 kb on + strand at 2418.971 kb on + strand at 2418.971 kb on + strand at 2418.972 kb on - strand at 2418.972 kb on - strand at 2418.972 kb on - strand at 2418.977 kb on - strand at 2418.977 kb on - strand at 2418.977 kb on - strand at 2418.977 kb on - strand at 2418.977 kb on - strand at 2418.985 kb on + strand at 2418.986 kb on - strand at 2418.986 kb on - strand at 2418.986 kb on - strand at 2419.092 kb on + strand at 2419.134 kb on + strand, within hemX at 2419.162 kb on + strand, within hemX at 2419.162 kb on + strand, within hemX at 2419.278 kb on + strand, within hemX at 2419.278 kb on + strand, within hemX at 2419.279 kb on - strand, within hemX at 2419.279 kb on - strand, within hemX at 2419.279 kb on - strand, within hemX at 2419.281 kb on + strand, within hemX at 2419.281 kb on + strand, within hemX at 2419.281 kb on + strand, within hemX at 2419.282 kb on - strand, within hemX at 2419.282 kb on - strand, within hemX at 2419.303 kb on - strand, within hemX at 2419.595 kb on - strand, within hemX at 2419.637 kb on + strand, within hemX at 2419.642 kb on - strand, within hemX at 2419.728 kb on + strand, within hemX at 2419.773 kb on + strand, within hemX at 2419.828 kb on - strand, within hemX at 2419.829 kb on + strand, within hemX at 2419.831 kb on + strand, within hemX at 2419.831 kb on + strand, within hemX at 2419.831 kb on + strand, within hemX at 2419.831 kb on + strand, within hemX at 2419.831 kb on + strand, within hemX at 2419.831 kb on + strand, within hemX at 2419.832 kb on - strand, within hemX at 2419.832 kb on - strand, within hemX at 2419.903 kb on + strand, within hemX at 2419.904 kb on - strand, within hemX at 2419.904 kb on - strand, within hemX at 2419.904 kb on - strand, within hemX at 2419.937 kb on + strand, within hemX at 2419.938 kb on - strand, within hemX at 2419.938 kb on - strand, within hemX at 2419.945 kb on + strand, within hemX at 2419.945 kb on + strand, within hemX at 2419.945 kb on + strand, within hemX at 2419.945 kb on + strand, within hemX at 2419.945 kb on + strand, within hemX at 2419.945 kb on + strand, within hemX at 2419.945 kb on + strand, within hemX at 2419.945 kb on + strand, within hemX at 2419.946 kb on - strand, within hemX at 2419.946 kb on - strand, within hemX at 2419.946 kb on - strand, within hemX at 2419.946 kb on - strand, within hemX at 2419.946 kb on - strand, within hemX at 2419.946 kb on - strand, within hemX at 2419.949 kb on - strand, within hemX at 2419.949 kb on - strand, within hemX at 2419.949 kb on - strand, within hemX at 2419.991 kb on + strand, within hemX at 2419.991 kb on + strand, within hemX at 2419.992 kb on - strand, within hemX at 2419.992 kb on - strand, within hemX at 2420.000 kb on + strand, within hemX at 2420.000 kb on + strand, within hemX at 2420.001 kb on - strand, within hemX at 2420.001 kb on - strand, within hemX at 2420.001 kb on - strand, within hemX at 2420.014 kb on - strand, within hemX at 2420.085 kb on + strand at 2420.125 kb on - strand at 2420.125 kb on - strand at 2420.125 kb on - strand at 2420.176 kb on - strand at 2420.203 kb on + strand at 2420.204 kb on - strand at 2420.213 kb on - strand at 2420.286 kb on + strand at 2420.286 kb on + strand at 2420.286 kb on + strand at 2420.286 kb on + strand at 2420.286 kb on + strand at 2420.287 kb on - strand at 2420.287 kb on - strand at 2420.287 kb on - strand at 2420.310 kb on + strand at 2420.310 kb on + strand at 2420.310 kb on + strand at 2420.310 kb on + strand at 2420.310 kb on + strand at 2420.310 kb on + strand at 2420.310 kb on + strand at 2420.310 kb on + strand at 2420.311 kb on - strand at 2420.311 kb on - strand at 2420.311 kb on - strand at 2420.311 kb on - strand at 2420.347 kb on + strand, within hemY at 2420.348 kb on - strand, within hemY at 2420.365 kb on + strand, within hemY at 2420.366 kb on - strand, within hemY at 2420.445 kb on - strand, within hemY at 2420.462 kb on + strand, within hemY at 2420.463 kb on - strand, within hemY at 2420.463 kb on - strand, within hemY at 2420.569 kb on - strand, within hemY at 2420.572 kb on + strand, within hemY at 2420.572 kb on + strand, within hemY at 2420.573 kb on - strand, within hemY at 2420.573 kb on - strand, within hemY at 2420.686 kb on + strand, within hemY at 2420.686 kb on + strand, within hemY at 2420.686 kb on + strand, within hemY at 2420.687 kb on - strand, within hemY at 2420.687 kb on - strand, within hemY at 2420.687 kb on - strand, within hemY at 2420.687 kb on - strand, within hemY at 2420.687 kb on - strand, within hemY at 2420.739 kb on - strand, within hemY at 2420.754 kb on - strand, within hemY at 2420.754 kb on - strand, within hemY at 2420.773 kb on + strand, within hemY at 2420.773 kb on + strand, within hemY at 2420.774 kb on - strand, within hemY at 2420.774 kb on - strand, within hemY at 2420.776 kb on + strand, within hemY at 2420.776 kb on + strand, within hemY at 2420.776 kb on + strand, within hemY at 2420.776 kb on + strand, within hemY at 2420.776 kb on + strand, within hemY at 2420.776 kb on + strand, within hemY at 2420.776 kb on + strand, within hemY at 2420.776 kb on + strand, within hemY at 2420.777 kb on - strand, within hemY at 2420.777 kb on - strand, within hemY at 2420.777 kb on - strand, within hemY at 2420.796 kb on + strand, within hemY at 2420.796 kb on + strand, within hemY at 2420.796 kb on + strand, within hemY at 2420.796 kb on + strand, within hemY at 2420.797 kb on - strand, within hemY at 2420.797 kb on - strand, within hemY at 2420.797 kb on - strand, within hemY at 2420.797 kb on - strand, within hemY at 2420.797 kb on - strand, within hemY at 2420.797 kb on - strand, within hemY at 2420.832 kb on - strand, within hemY at 2420.834 kb on + strand, within hemY at 2420.835 kb on - strand, within hemY at 2420.996 kb on - strand, within hemY at 2421.026 kb on + strand, within hemY at 2421.027 kb on - strand, within hemY at 2421.047 kb on + strand, within hemY at 2421.048 kb on - strand, within hemY
Per-strain Table
Position Strand Gene LocusTag Fraction Bas14 remove 2,418,218 + +1.0 2,418,218 + -1.4 2,418,218 + -1.6 2,418,218 + +3.6 2,418,962 + +4.0 2,418,971 + +0.6 2,418,971 + -1.1 2,418,971 + +1.5 2,418,971 + +1.2 2,418,971 + -0.4 2,418,971 + -0.2 2,418,972 - +1.0 2,418,972 - +0.2 2,418,972 - +0.8 2,418,977 - +0.8 2,418,977 - +1.6 2,418,977 - +0.4 2,418,977 - -1.0 2,418,977 - +0.5 2,418,985 + +2.1 2,418,986 - -1.0 2,418,986 - +0.8 2,418,986 - +0.6 2,419,092 + +0.2 2,419,134 + hemX NIAGMN_12215 0.11 +0.0 2,419,162 + hemX NIAGMN_12215 0.13 +2.3 2,419,162 + hemX NIAGMN_12215 0.13 -1.1 2,419,278 + hemX NIAGMN_12215 0.23 +2.0 2,419,278 + hemX NIAGMN_12215 0.23 -1.5 2,419,279 - hemX NIAGMN_12215 0.23 +0.5 2,419,279 - hemX NIAGMN_12215 0.23 +1.0 2,419,279 - hemX NIAGMN_12215 0.23 +0.2 2,419,281 + hemX NIAGMN_12215 0.23 -1.0 2,419,281 + hemX NIAGMN_12215 0.23 +2.0 2,419,281 + hemX NIAGMN_12215 0.23 +0.0 2,419,282 - hemX NIAGMN_12215 0.23 -1.4 2,419,282 - hemX NIAGMN_12215 0.23 +3.0 2,419,303 - hemX NIAGMN_12215 0.25 -1.8 2,419,595 - hemX NIAGMN_12215 0.49 +0.9 2,419,637 + hemX NIAGMN_12215 0.53 -0.4 2,419,642 - hemX NIAGMN_12215 0.53 -0.1 2,419,728 + hemX NIAGMN_12215 0.60 -1.7 2,419,773 + hemX NIAGMN_12215 0.64 +0.5 2,419,828 - hemX NIAGMN_12215 0.69 +0.3 2,419,829 + hemX NIAGMN_12215 0.69 -1.0 2,419,831 + hemX NIAGMN_12215 0.69 -1.0 2,419,831 + hemX NIAGMN_12215 0.69 +1.0 2,419,831 + hemX NIAGMN_12215 0.69 +0.6 2,419,831 + hemX NIAGMN_12215 0.69 -1.2 2,419,831 + hemX NIAGMN_12215 0.69 +0.5 2,419,831 + hemX NIAGMN_12215 0.69 -0.7 2,419,832 - hemX NIAGMN_12215 0.69 +0.5 2,419,832 - hemX NIAGMN_12215 0.69 +0.5 2,419,903 + hemX NIAGMN_12215 0.75 +0.8 2,419,904 - hemX NIAGMN_12215 0.75 -1.5 2,419,904 - hemX NIAGMN_12215 0.75 +0.3 2,419,904 - hemX NIAGMN_12215 0.75 +1.0 2,419,937 + hemX NIAGMN_12215 0.78 -0.7 2,419,938 - hemX NIAGMN_12215 0.78 -1.0 2,419,938 - hemX NIAGMN_12215 0.78 -2.1 2,419,945 + hemX NIAGMN_12215 0.78 -0.4 2,419,945 + hemX NIAGMN_12215 0.78 +0.9 2,419,945 + hemX NIAGMN_12215 0.78 +0.7 2,419,945 + hemX NIAGMN_12215 0.78 -1.5 2,419,945 + hemX NIAGMN_12215 0.78 +0.6 2,419,945 + hemX NIAGMN_12215 0.78 +0.5 2,419,945 + hemX NIAGMN_12215 0.78 +1.0 2,419,945 + hemX NIAGMN_12215 0.78 -2.0 2,419,946 - hemX NIAGMN_12215 0.78 -1.1 2,419,946 - hemX NIAGMN_12215 0.78 -0.1 2,419,946 - hemX NIAGMN_12215 0.78 +0.5 2,419,946 - hemX NIAGMN_12215 0.78 +1.0 2,419,946 - hemX NIAGMN_12215 0.78 -0.7 2,419,946 - hemX NIAGMN_12215 0.78 +1.0 2,419,949 - hemX NIAGMN_12215 0.79 -1.3 2,419,949 - hemX NIAGMN_12215 0.79 +2.0 2,419,949 - hemX NIAGMN_12215 0.79 +0.6 2,419,991 + hemX NIAGMN_12215 0.82 -2.4 2,419,991 + hemX NIAGMN_12215 0.82 +1.2 2,419,992 - hemX NIAGMN_12215 0.82 +1.2 2,419,992 - hemX NIAGMN_12215 0.82 -0.9 2,420,000 + hemX NIAGMN_12215 0.83 -1.0 2,420,000 + hemX NIAGMN_12215 0.83 -1.9 2,420,001 - hemX NIAGMN_12215 0.83 +0.5 2,420,001 - hemX NIAGMN_12215 0.83 -1.4 2,420,001 - hemX NIAGMN_12215 0.83 +0.5 2,420,014 - hemX NIAGMN_12215 0.84 +0.9 2,420,085 + -0.3 2,420,125 - -0.5 2,420,125 - -0.1 2,420,125 - -0.2 2,420,176 - +0.6 2,420,203 + -1.9 2,420,204 - +1.0 2,420,213 - +1.3 2,420,286 + -0.1 2,420,286 + -0.4 2,420,286 + -0.5 2,420,286 + +2.0 2,420,286 + -0.3 2,420,287 - +2.0 2,420,287 - -0.3 2,420,287 - -1.3 2,420,310 + +0.5 2,420,310 + -0.7 2,420,310 + +2.0 2,420,310 + -1.1 2,420,310 + +1.5 2,420,310 + -0.0 2,420,310 + -0.5 2,420,310 + +1.4 2,420,311 - +0.6 2,420,311 - +2.0 2,420,311 - +0.5 2,420,311 - +0.5 2,420,347 + hemY NIAGMN_12220 0.12 +2.0 2,420,348 - hemY NIAGMN_12220 0.12 -0.4 2,420,365 + hemY NIAGMN_12220 0.13 -0.0 2,420,366 - hemY NIAGMN_12220 0.13 -0.2 2,420,445 - hemY NIAGMN_12220 0.20 -0.5 2,420,462 + hemY NIAGMN_12220 0.21 -0.1 2,420,463 - hemY NIAGMN_12220 0.21 -2.4 2,420,463 - hemY NIAGMN_12220 0.21 -1.5 2,420,569 - hemY NIAGMN_12220 0.30 -1.8 2,420,572 + hemY NIAGMN_12220 0.30 +0.0 2,420,572 + hemY NIAGMN_12220 0.30 +0.0 2,420,573 - hemY NIAGMN_12220 0.30 +1.3 2,420,573 - hemY NIAGMN_12220 0.30 -0.2 2,420,686 + hemY NIAGMN_12220 0.40 -0.9 2,420,686 + hemY NIAGMN_12220 0.40 -0.9 2,420,686 + hemY NIAGMN_12220 0.40 -0.8 2,420,687 - hemY NIAGMN_12220 0.40 +0.9 2,420,687 - hemY NIAGMN_12220 0.40 -1.0 2,420,687 - hemY NIAGMN_12220 0.40 +0.5 2,420,687 - hemY NIAGMN_12220 0.40 -0.9 2,420,687 - hemY NIAGMN_12220 0.40 +0.9 2,420,739 - hemY NIAGMN_12220 0.44 -1.1 2,420,754 - hemY NIAGMN_12220 0.46 -1.7 2,420,754 - hemY NIAGMN_12220 0.46 +0.5 2,420,773 + hemY NIAGMN_12220 0.47 -1.1 2,420,773 + hemY NIAGMN_12220 0.47 +0.2 2,420,774 - hemY NIAGMN_12220 0.47 +1.0 2,420,774 - hemY NIAGMN_12220 0.47 -0.8 2,420,776 + hemY NIAGMN_12220 0.47 -0.4 2,420,776 + hemY NIAGMN_12220 0.47 +0.7 2,420,776 + hemY NIAGMN_12220 0.47 +0.5 2,420,776 + hemY NIAGMN_12220 0.47 +0.1 2,420,776 + hemY NIAGMN_12220 0.47 -1.0 2,420,776 + hemY NIAGMN_12220 0.47 -1.2 2,420,776 + hemY NIAGMN_12220 0.47 -1.4 2,420,776 + hemY NIAGMN_12220 0.47 +0.3 2,420,777 - hemY NIAGMN_12220 0.48 -1.2 2,420,777 - hemY NIAGMN_12220 0.48 +0.3 2,420,777 - hemY NIAGMN_12220 0.48 +0.5 2,420,796 + hemY NIAGMN_12220 0.49 +0.0 2,420,796 + hemY NIAGMN_12220 0.49 -1.5 2,420,796 + hemY NIAGMN_12220 0.49 -0.4 2,420,796 + hemY NIAGMN_12220 0.49 +0.5 2,420,797 - hemY NIAGMN_12220 0.49 +1.0 2,420,797 - hemY NIAGMN_12220 0.49 +0.3 2,420,797 - hemY NIAGMN_12220 0.49 -0.1 2,420,797 - hemY NIAGMN_12220 0.49 +0.6 2,420,797 - hemY NIAGMN_12220 0.49 +1.2 2,420,797 - hemY NIAGMN_12220 0.49 +0.5 2,420,832 - hemY NIAGMN_12220 0.52 +0.0 2,420,834 + hemY NIAGMN_12220 0.52 +0.2 2,420,835 - hemY NIAGMN_12220 0.52 +2.0 2,420,996 - hemY NIAGMN_12220 0.66 -0.8 2,421,026 + hemY NIAGMN_12220 0.68 -0.3 2,421,027 - hemY NIAGMN_12220 0.68 -1.6 2,421,047 + hemY NIAGMN_12220 0.70 +2.0 2,421,048 - hemY NIAGMN_12220 0.70 -0.1
Or see this region's nucleotide sequence