Experiment: Bas14
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nanS and NIAGMN_09485 are separated by 295 nucleotides NIAGMN_09485 and NIAGMN_09490 are separated by 101 nucleotides NIAGMN_09490 and NIAGMN_09495 are separated by 159 nucleotides NIAGMN_09495 and NIAGMN_09500 are separated by 369 nucleotides NIAGMN_09500 and uvrD are separated by 128 nucleotides
NIAGMN_09480: nanS - putative 9-O-acetyl-N-acetylneuraminic acid deacetylase, at 1,847,994 to 1,848,974
nanS
NIAGMN_09485: NIAGMN_09485 - Transposase, IS4 family protein, at 1,849,270 to 1,849,515
_09485
NIAGMN_09490: NIAGMN_09490 - Transposase, at 1,849,617 to 1,849,703
_09490
NIAGMN_09495: NIAGMN_09495 - Orf, at 1,849,863 to 1,849,901
_09495
NIAGMN_09500: NIAGMN_09500 - Orf, at 1,850,271 to 1,850,339
_09500
NIAGMN_09505: uvrD - DNA helicase, at 1,850,468 to 1,852,582
uvrD
Position (kb)
1849
1850 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1848.924 kb on - strand at 1848.940 kb on + strand at 1848.941 kb on - strand at 1849.037 kb on + strand at 1849.037 kb on + strand at 1849.038 kb on - strand at 1849.038 kb on - strand at 1849.073 kb on - strand at 1849.111 kb on + strand at 1849.111 kb on + strand at 1849.149 kb on + strand at 1849.149 kb on + strand at 1849.333 kb on + strand, within NIAGMN_09485 at 1849.421 kb on - strand, within NIAGMN_09485 at 1849.423 kb on + strand, within NIAGMN_09485 at 1849.423 kb on + strand, within NIAGMN_09485 at 1849.423 kb on + strand, within NIAGMN_09485 at 1849.510 kb on + strand at 1849.510 kb on + strand at 1849.510 kb on + strand at 1849.511 kb on - strand at 1849.586 kb on + strand at 1849.586 kb on + strand at 1849.586 kb on + strand at 1849.605 kb on - strand at 1849.653 kb on - strand, within NIAGMN_09490 at 1849.787 kb on - strand at 1849.855 kb on + strand at 1849.938 kb on + strand at 1850.081 kb on + strand at 1850.239 kb on - strand at 1850.300 kb on + strand, within NIAGMN_09500 at 1850.394 kb on + strand at 1850.423 kb on - strand at 1850.424 kb on + strand at 1850.467 kb on - strand at 1850.480 kb on + strand at 1850.481 kb on - strand at 1850.481 kb on - strand at 1850.526 kb on - strand at 1850.566 kb on + strand at 1850.566 kb on + strand at 1850.571 kb on - strand at 1850.607 kb on - strand at 1850.638 kb on + strand at 1850.658 kb on + strand at 1850.658 kb on + strand at 1850.681 kb on - strand, within uvrD at 1850.746 kb on + strand, within uvrD at 1850.747 kb on - strand, within uvrD at 1850.747 kb on - strand, within uvrD at 1850.747 kb on - strand, within uvrD at 1850.804 kb on - strand, within uvrD at 1850.804 kb on - strand, within uvrD at 1850.807 kb on - strand, within uvrD at 1850.825 kb on - strand, within uvrD
Per-strain Table
Position Strand Gene LocusTag Fraction Bas14 remove 1,848,924 - -0.3 1,848,940 + -0.8 1,848,941 - -0.1 1,849,037 + -1.0 1,849,037 + -1.0 1,849,038 - -1.6 1,849,038 - -0.7 1,849,073 - -2.6 1,849,111 + -0.2 1,849,111 + -0.0 1,849,149 + +1.2 1,849,149 + -0.2 1,849,333 + NIAGMN_09485 0.26 -0.2 1,849,421 - NIAGMN_09485 0.61 +0.3 1,849,423 + NIAGMN_09485 0.62 -0.4 1,849,423 + NIAGMN_09485 0.62 -1.3 1,849,423 + NIAGMN_09485 0.62 -1.0 1,849,510 + -0.2 1,849,510 + -1.6 1,849,510 + -1.0 1,849,511 - -1.0 1,849,586 + -0.8 1,849,586 + -0.9 1,849,586 + -0.5 1,849,605 - -0.7 1,849,653 - NIAGMN_09490 0.41 +0.2 1,849,787 - +1.5 1,849,855 + +0.3 1,849,938 + -0.6 1,850,081 + -1.5 1,850,239 - -1.5 1,850,300 + NIAGMN_09500 0.42 -0.8 1,850,394 + +1.2 1,850,423 - -0.3 1,850,424 + -0.3 1,850,467 - -0.7 1,850,480 + -1.2 1,850,481 - +1.3 1,850,481 - -0.6 1,850,526 - +0.8 1,850,566 + +2.0 1,850,566 + +0.7 1,850,571 - +0.4 1,850,607 - -1.0 1,850,638 + -0.5 1,850,658 + +0.6 1,850,658 + -1.9 1,850,681 - uvrD NIAGMN_09505 0.10 +1.0 1,850,746 + uvrD NIAGMN_09505 0.13 -1.0 1,850,747 - uvrD NIAGMN_09505 0.13 -1.5 1,850,747 - uvrD NIAGMN_09505 0.13 +0.2 1,850,747 - uvrD NIAGMN_09505 0.13 -1.8 1,850,804 - uvrD NIAGMN_09505 0.16 +1.0 1,850,804 - uvrD NIAGMN_09505 0.16 -0.3 1,850,807 - uvrD NIAGMN_09505 0.16 +3.0 1,850,825 - uvrD NIAGMN_09505 0.17 +0.9
Or see this region's nucleotide sequence