Experiment: Bas14
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hemL and fhuB are separated by 34 nucleotides fhuB and fhuD overlap by 4 nucleotides fhuD and fhuC overlap by 1 nucleotides
NIAGMN_08150: hemL - glutamate-1-semialdehyde 2,1-aminomutase, at 1,562,963 to 1,564,243
hemL
NIAGMN_08155: fhuB - Fe(3+)-hydroxamate ABC transporter permease FhuB, at 1,564,278 to 1,566,260
fhuB
NIAGMN_08160: fhuD - Fe(3+)-hydroxamate ABC transporter substrate-binding protein FhuD, at 1,566,257 to 1,567,147
fhuD
NIAGMN_08165: fhuC - Fe3+-hydroxamate ABC transporter ATP-binding protein FhuC, at 1,567,147 to 1,567,944
fhuC
Position (kb)
1564
1565
1566
1567 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1564.276 kb on + strand at 1564.280 kb on - strand at 1564.303 kb on + strand at 1564.304 kb on - strand at 1564.307 kb on + strand at 1564.308 kb on - strand at 1564.308 kb on - strand at 1564.486 kb on - strand, within fhuB at 1564.486 kb on - strand, within fhuB at 1564.618 kb on - strand, within fhuB at 1564.780 kb on - strand, within fhuB at 1564.799 kb on + strand, within fhuB at 1564.800 kb on - strand, within fhuB at 1564.803 kb on + strand, within fhuB at 1564.804 kb on - strand, within fhuB at 1564.804 kb on - strand, within fhuB at 1564.865 kb on + strand, within fhuB at 1564.896 kb on - strand, within fhuB at 1564.980 kb on - strand, within fhuB at 1564.980 kb on - strand, within fhuB at 1564.980 kb on - strand, within fhuB at 1564.980 kb on - strand, within fhuB at 1565.034 kb on - strand, within fhuB at 1565.034 kb on - strand, within fhuB at 1565.274 kb on - strand, within fhuB at 1565.274 kb on - strand, within fhuB at 1565.284 kb on - strand, within fhuB at 1565.304 kb on + strand, within fhuB at 1565.304 kb on + strand, within fhuB at 1565.305 kb on - strand, within fhuB at 1565.305 kb on - strand, within fhuB at 1565.541 kb on - strand, within fhuB at 1565.541 kb on - strand, within fhuB at 1565.637 kb on + strand, within fhuB at 1565.795 kb on - strand, within fhuB at 1565.795 kb on - strand, within fhuB at 1565.806 kb on - strand, within fhuB at 1565.871 kb on + strand, within fhuB at 1566.009 kb on - strand, within fhuB at 1566.126 kb on - strand at 1566.229 kb on - strand at 1566.249 kb on - strand at 1566.249 kb on - strand at 1566.249 kb on - strand at 1566.255 kb on - strand at 1566.324 kb on + strand at 1566.325 kb on - strand at 1566.325 kb on - strand at 1566.458 kb on - strand, within fhuD at 1566.459 kb on + strand, within fhuD at 1566.460 kb on - strand, within fhuD at 1566.460 kb on - strand, within fhuD at 1566.460 kb on - strand, within fhuD at 1566.460 kb on - strand, within fhuD at 1566.490 kb on + strand, within fhuD at 1566.491 kb on - strand, within fhuD at 1566.491 kb on - strand, within fhuD at 1566.587 kb on - strand, within fhuD at 1566.587 kb on - strand, within fhuD at 1566.676 kb on - strand, within fhuD at 1566.713 kb on - strand, within fhuD at 1566.743 kb on - strand, within fhuD at 1566.851 kb on - strand, within fhuD at 1566.887 kb on - strand, within fhuD at 1566.927 kb on - strand, within fhuD at 1566.927 kb on - strand, within fhuD at 1567.001 kb on + strand, within fhuD at 1567.002 kb on - strand, within fhuD at 1567.004 kb on + strand, within fhuD at 1567.004 kb on + strand, within fhuD at 1567.005 kb on - strand, within fhuD at 1567.005 kb on - strand, within fhuD at 1567.036 kb on + strand, within fhuD at 1567.036 kb on + strand, within fhuD at 1567.051 kb on + strand, within fhuD at 1567.082 kb on + strand at 1567.152 kb on - strand at 1567.212 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bas14 remove 1,564,276 + -0.0 1,564,280 - -0.5 1,564,303 + +1.3 1,564,304 - +1.3 1,564,307 + +1.3 1,564,308 - +0.4 1,564,308 - +1.6 1,564,486 - fhuB NIAGMN_08155 0.10 +0.1 1,564,486 - fhuB NIAGMN_08155 0.10 +1.4 1,564,618 - fhuB NIAGMN_08155 0.17 -1.7 1,564,780 - fhuB NIAGMN_08155 0.25 -1.4 1,564,799 + fhuB NIAGMN_08155 0.26 +0.3 1,564,800 - fhuB NIAGMN_08155 0.26 -0.5 1,564,803 + fhuB NIAGMN_08155 0.26 +1.9 1,564,804 - fhuB NIAGMN_08155 0.27 +0.3 1,564,804 - fhuB NIAGMN_08155 0.27 -1.4 1,564,865 + fhuB NIAGMN_08155 0.30 +1.9 1,564,896 - fhuB NIAGMN_08155 0.31 -0.2 1,564,980 - fhuB NIAGMN_08155 0.35 +0.6 1,564,980 - fhuB NIAGMN_08155 0.35 -0.4 1,564,980 - fhuB NIAGMN_08155 0.35 +0.2 1,564,980 - fhuB NIAGMN_08155 0.35 -0.1 1,565,034 - fhuB NIAGMN_08155 0.38 -0.4 1,565,034 - fhuB NIAGMN_08155 0.38 -0.7 1,565,274 - fhuB NIAGMN_08155 0.50 +0.2 1,565,274 - fhuB NIAGMN_08155 0.50 +1.8 1,565,284 - fhuB NIAGMN_08155 0.51 -0.1 1,565,304 + fhuB NIAGMN_08155 0.52 -0.5 1,565,304 + fhuB NIAGMN_08155 0.52 +0.9 1,565,305 - fhuB NIAGMN_08155 0.52 -1.1 1,565,305 - fhuB NIAGMN_08155 0.52 -0.2 1,565,541 - fhuB NIAGMN_08155 0.64 -0.4 1,565,541 - fhuB NIAGMN_08155 0.64 -0.6 1,565,637 + fhuB NIAGMN_08155 0.69 +0.9 1,565,795 - fhuB NIAGMN_08155 0.77 -0.2 1,565,795 - fhuB NIAGMN_08155 0.77 -1.3 1,565,806 - fhuB NIAGMN_08155 0.77 +0.3 1,565,871 + fhuB NIAGMN_08155 0.80 +0.3 1,566,009 - fhuB NIAGMN_08155 0.87 +0.8 1,566,126 - +0.4 1,566,229 - +0.7 1,566,249 - +0.1 1,566,249 - -0.2 1,566,249 - -1.1 1,566,255 - -0.7 1,566,324 + +1.9 1,566,325 - +0.7 1,566,325 - -2.4 1,566,458 - fhuD NIAGMN_08160 0.23 -1.0 1,566,459 + fhuD NIAGMN_08160 0.23 -0.1 1,566,460 - fhuD NIAGMN_08160 0.23 +0.0 1,566,460 - fhuD NIAGMN_08160 0.23 -0.1 1,566,460 - fhuD NIAGMN_08160 0.23 -0.4 1,566,460 - fhuD NIAGMN_08160 0.23 -1.0 1,566,490 + fhuD NIAGMN_08160 0.26 +1.6 1,566,491 - fhuD NIAGMN_08160 0.26 +0.5 1,566,491 - fhuD NIAGMN_08160 0.26 +0.9 1,566,587 - fhuD NIAGMN_08160 0.37 -0.8 1,566,587 - fhuD NIAGMN_08160 0.37 -0.1 1,566,676 - fhuD NIAGMN_08160 0.47 +0.5 1,566,713 - fhuD NIAGMN_08160 0.51 -1.8 1,566,743 - fhuD NIAGMN_08160 0.55 +1.0 1,566,851 - fhuD NIAGMN_08160 0.67 +0.3 1,566,887 - fhuD NIAGMN_08160 0.71 +0.1 1,566,927 - fhuD NIAGMN_08160 0.75 -0.0 1,566,927 - fhuD NIAGMN_08160 0.75 -0.7 1,567,001 + fhuD NIAGMN_08160 0.84 -1.3 1,567,002 - fhuD NIAGMN_08160 0.84 -0.7 1,567,004 + fhuD NIAGMN_08160 0.84 -3.4 1,567,004 + fhuD NIAGMN_08160 0.84 -0.5 1,567,005 - fhuD NIAGMN_08160 0.84 -1.2 1,567,005 - fhuD NIAGMN_08160 0.84 +1.8 1,567,036 + fhuD NIAGMN_08160 0.87 -0.8 1,567,036 + fhuD NIAGMN_08160 0.87 +1.0 1,567,051 + fhuD NIAGMN_08160 0.89 -1.0 1,567,082 + +0.1 1,567,152 - +2.5 1,567,212 - -0.2
Or see this region's nucleotide sequence