Experiment: Bas14
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ybaA and atl are separated by 378 nucleotides atl and ybaY are separated by 32 nucleotides ybaY and tesB are separated by 217 nucleotides
NIAGMN_06290: ybaA - Uncharacterized protein YbaA, at 1,197,641 to 1,197,994
ybaA
NIAGMN_06300: atl - DNA base-flipping protein, at 1,198,373 to 1,198,684
atl
NIAGMN_06305: ybaY - Uncharacterized lipoprotein YbaY, at 1,198,717 to 1,199,289
ybaY
NIAGMN_06310: tesB - acyl-CoA thioesterase II, at 1,199,507 to 1,200,367
tesB
Position (kb)
1198
1199
1200 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1197.778 kb on - strand, within ybaA at 1197.988 kb on - strand at 1198.002 kb on - strand at 1198.015 kb on - strand at 1198.015 kb on - strand at 1198.436 kb on + strand, within atl at 1198.436 kb on + strand, within atl at 1198.448 kb on + strand, within atl at 1198.510 kb on - strand, within atl at 1198.593 kb on + strand, within atl at 1198.593 kb on + strand, within atl at 1198.659 kb on + strand at 1198.659 kb on + strand at 1198.679 kb on + strand at 1198.679 kb on + strand at 1198.679 kb on + strand at 1198.680 kb on - strand at 1198.680 kb on - strand at 1198.682 kb on + strand at 1198.682 kb on + strand at 1198.711 kb on + strand at 1198.712 kb on - strand at 1198.718 kb on + strand at 1198.718 kb on + strand at 1198.718 kb on + strand at 1198.719 kb on - strand at 1198.721 kb on + strand at 1198.721 kb on + strand at 1198.721 kb on + strand at 1198.722 kb on - strand at 1198.722 kb on - strand at 1198.780 kb on + strand, within ybaY at 1198.786 kb on - strand, within ybaY at 1198.900 kb on - strand, within ybaY at 1198.904 kb on - strand, within ybaY at 1198.945 kb on - strand, within ybaY at 1198.974 kb on + strand, within ybaY at 1198.975 kb on - strand, within ybaY at 1199.007 kb on + strand, within ybaY at 1199.008 kb on - strand, within ybaY at 1199.157 kb on + strand, within ybaY at 1199.188 kb on - strand, within ybaY at 1199.188 kb on - strand, within ybaY at 1199.302 kb on - strand at 1199.305 kb on - strand at 1199.305 kb on - strand at 1199.359 kb on + strand at 1199.360 kb on - strand at 1199.457 kb on + strand at 1199.457 kb on + strand at 1199.457 kb on + strand at 1199.458 kb on - strand at 1199.504 kb on + strand at 1199.504 kb on + strand at 1199.505 kb on - strand at 1199.505 kb on - strand at 1199.505 kb on - strand at 1199.507 kb on - strand at 1199.592 kb on + strand at 1199.608 kb on - strand, within tesB at 1199.639 kb on + strand, within tesB at 1199.639 kb on + strand, within tesB at 1199.639 kb on + strand, within tesB at 1199.640 kb on - strand, within tesB at 1199.676 kb on + strand, within tesB at 1199.716 kb on + strand, within tesB at 1199.716 kb on + strand, within tesB at 1199.717 kb on - strand, within tesB at 1199.717 kb on - strand, within tesB at 1199.729 kb on - strand, within tesB at 1199.732 kb on + strand, within tesB at 1199.732 kb on + strand, within tesB at 1199.813 kb on + strand, within tesB at 1199.814 kb on - strand, within tesB at 1200.095 kb on + strand, within tesB at 1200.096 kb on - strand, within tesB at 1200.121 kb on + strand, within tesB at 1200.136 kb on - strand, within tesB at 1200.141 kb on + strand, within tesB at 1200.245 kb on + strand, within tesB at 1200.245 kb on + strand, within tesB at 1200.247 kb on + strand, within tesB
Per-strain Table
Position Strand Gene LocusTag Fraction Bas14 remove 1,197,778 - ybaA NIAGMN_06290 0.39 +0.4 1,197,988 - -1.0 1,198,002 - +0.5 1,198,015 - -0.8 1,198,015 - +0.3 1,198,436 + atl NIAGMN_06300 0.20 +0.5 1,198,436 + atl NIAGMN_06300 0.20 -0.2 1,198,448 + atl NIAGMN_06300 0.24 +0.4 1,198,510 - atl NIAGMN_06300 0.44 +0.8 1,198,593 + atl NIAGMN_06300 0.71 -1.2 1,198,593 + atl NIAGMN_06300 0.71 +1.8 1,198,659 + +0.8 1,198,659 + -0.5 1,198,679 + +0.1 1,198,679 + -1.2 1,198,679 + -1.9 1,198,680 - +1.4 1,198,680 - +0.5 1,198,682 + +0.3 1,198,682 + -1.2 1,198,711 + +0.2 1,198,712 - +0.4 1,198,718 + -0.7 1,198,718 + +0.7 1,198,718 + +0.3 1,198,719 - +0.1 1,198,721 + +1.8 1,198,721 + +1.5 1,198,721 + -0.2 1,198,722 - +0.8 1,198,722 - -2.0 1,198,780 + ybaY NIAGMN_06305 0.11 -1.5 1,198,786 - ybaY NIAGMN_06305 0.12 +1.2 1,198,900 - ybaY NIAGMN_06305 0.32 +1.3 1,198,904 - ybaY NIAGMN_06305 0.33 -1.9 1,198,945 - ybaY NIAGMN_06305 0.40 +1.1 1,198,974 + ybaY NIAGMN_06305 0.45 +1.8 1,198,975 - ybaY NIAGMN_06305 0.45 -1.2 1,199,007 + ybaY NIAGMN_06305 0.51 +0.8 1,199,008 - ybaY NIAGMN_06305 0.51 -1.5 1,199,157 + ybaY NIAGMN_06305 0.77 +1.3 1,199,188 - ybaY NIAGMN_06305 0.82 +0.8 1,199,188 - ybaY NIAGMN_06305 0.82 -1.0 1,199,302 - +1.4 1,199,305 - +2.4 1,199,305 - -0.2 1,199,359 + +0.6 1,199,360 - +0.3 1,199,457 + -0.2 1,199,457 + +0.8 1,199,457 + -0.7 1,199,458 - +0.5 1,199,504 + +0.7 1,199,504 + +3.2 1,199,505 - -0.4 1,199,505 - +0.0 1,199,505 - -0.5 1,199,507 - +0.4 1,199,592 + +1.0 1,199,608 - tesB NIAGMN_06310 0.12 +0.0 1,199,639 + tesB NIAGMN_06310 0.15 +1.3 1,199,639 + tesB NIAGMN_06310 0.15 +0.5 1,199,639 + tesB NIAGMN_06310 0.15 -0.2 1,199,640 - tesB NIAGMN_06310 0.15 +2.4 1,199,676 + tesB NIAGMN_06310 0.20 -1.6 1,199,716 + tesB NIAGMN_06310 0.24 -1.2 1,199,716 + tesB NIAGMN_06310 0.24 -1.6 1,199,717 - tesB NIAGMN_06310 0.24 +0.8 1,199,717 - tesB NIAGMN_06310 0.24 -0.5 1,199,729 - tesB NIAGMN_06310 0.26 -0.4 1,199,732 + tesB NIAGMN_06310 0.26 -0.6 1,199,732 + tesB NIAGMN_06310 0.26 -1.5 1,199,813 + tesB NIAGMN_06310 0.36 -0.7 1,199,814 - tesB NIAGMN_06310 0.36 +0.4 1,200,095 + tesB NIAGMN_06310 0.68 -0.7 1,200,096 - tesB NIAGMN_06310 0.68 +0.2 1,200,121 + tesB NIAGMN_06310 0.71 +0.6 1,200,136 - tesB NIAGMN_06310 0.73 -1.0 1,200,141 + tesB NIAGMN_06310 0.74 -0.9 1,200,245 + tesB NIAGMN_06310 0.86 +0.8 1,200,245 + tesB NIAGMN_06310 0.86 -1.8 1,200,247 + tesB NIAGMN_06310 0.86 +2.4
Or see this region's nucleotide sequence