Experiment: Bas14
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt zitB and pnuC overlap by 4 nucleotides pnuC and nadA are separated by 37 nucleotides nadA and Lys_trna are separated by 277 nucleotides Lys_trna and Lys_trna are separated by 146 nucleotides Lys_trna and Val_trna are separated by 3 nucleotides Val_trna and Lys_trna are separated by 51 nucleotides Lys_trna and Val_trna are separated by 2 nucleotides Val_trna and Lys_trna are separated by 35 nucleotides
NIAGMN_04800: zitB - CDF family zinc transporter ZitB, at 872,353 to 873,294
zitB
NIAGMN_04805: pnuC - nicotinamide riboside transporter PnuC, at 873,291 to 874,010
pnuC
NIAGMN_04810: nadA - quinolinate synthase NadA, at 874,048 to 875,091
nadA
NIAGMN_04815: Lys_trna - tRNA-Lys(ttt), at 875,369 to 875,444
_trna
NIAGMN_04820: Lys_trna - tRNA-Lys(ttt), at 875,591 to 875,666
_trna
NIAGMN_04825: Val_trna - tRNA-Val(tac), at 875,670 to 875,745
_trna
NIAGMN_04830: Lys_trna - tRNA-Lys(ttt), at 875,797 to 875,872
_trna
NIAGMN_04835: Val_trna - tRNA-Val(tac), at 875,875 to 875,950
_trna
NIAGMN_04840: Lys_trna - tRNA-Lys(ttt), at 875,986 to 876,061
_trna
Position (kb) 874
875
876 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 873.083 kb on + strand, within zitB at 873.083 kb on + strand, within zitB at 873.083 kb on + strand, within zitB at 873.190 kb on + strand, within zitB at 873.223 kb on + strand at 873.223 kb on + strand at 873.380 kb on - strand, within pnuC at 873.428 kb on + strand, within pnuC at 873.441 kb on - strand, within pnuC at 873.473 kb on + strand, within pnuC at 873.474 kb on - strand, within pnuC at 873.504 kb on + strand, within pnuC at 873.504 kb on + strand, within pnuC at 873.504 kb on + strand, within pnuC at 873.550 kb on - strand, within pnuC at 873.793 kb on - strand, within pnuC at 873.808 kb on + strand, within pnuC at 873.972 kb on + strand at 873.973 kb on - strand at 873.973 kb on - strand at 873.974 kb on + strand at 873.975 kb on - strand at 873.975 kb on - strand at 873.975 kb on - strand at 873.975 kb on - strand at 873.975 kb on - strand at 873.979 kb on - strand at 873.979 kb on - strand at 874.043 kb on - strand at 874.058 kb on - strand at 874.081 kb on - strand at 874.156 kb on + strand, within nadA at 874.157 kb on - strand, within nadA at 874.396 kb on - strand, within nadA at 874.510 kb on + strand, within nadA at 874.841 kb on - strand, within nadA at 874.899 kb on + strand, within nadA at 875.220 kb on + strand at 875.264 kb on + strand at 875.293 kb on - strand at 875.335 kb on - strand at 875.352 kb on - strand at 875.365 kb on - strand at 875.471 kb on - strand at 875.567 kb on - strand at 875.567 kb on - strand at 875.749 kb on - strand at 875.761 kb on - strand at 875.761 kb on - strand at 875.764 kb on - strand at 875.771 kb on - strand at 875.771 kb on - strand at 875.791 kb on - strand at 875.791 kb on - strand at 875.795 kb on - strand at 875.795 kb on - strand at 875.957 kb on - strand at 875.957 kb on - strand at 875.957 kb on - strand at 875.969 kb on - strand at 875.969 kb on - strand at 875.969 kb on - strand at 875.975 kb on - strand at 875.975 kb on - strand at 875.975 kb on - strand at 875.975 kb on - strand at 875.979 kb on - strand at 875.979 kb on - strand at 875.979 kb on - strand at 875.979 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bas14 remove 873,083 + zitB NIAGMN_04800 0.77 +0.3 873,083 + zitB NIAGMN_04800 0.77 +1.3 873,083 + zitB NIAGMN_04800 0.77 +0.3 873,190 + zitB NIAGMN_04800 0.89 -0.9 873,223 + -1.6 873,223 + +1.2 873,380 - pnuC NIAGMN_04805 0.12 +0.9 873,428 + pnuC NIAGMN_04805 0.19 +1.0 873,441 - pnuC NIAGMN_04805 0.21 +1.7 873,473 + pnuC NIAGMN_04805 0.25 -2.1 873,474 - pnuC NIAGMN_04805 0.25 -0.6 873,504 + pnuC NIAGMN_04805 0.30 +0.2 873,504 + pnuC NIAGMN_04805 0.30 +1.7 873,504 + pnuC NIAGMN_04805 0.30 +0.7 873,550 - pnuC NIAGMN_04805 0.36 -0.6 873,793 - pnuC NIAGMN_04805 0.70 +0.7 873,808 + pnuC NIAGMN_04805 0.72 -2.5 873,972 + -0.6 873,973 - +0.7 873,973 - +1.7 873,974 + -0.2 873,975 - +0.7 873,975 - +0.6 873,975 - +1.2 873,975 - +0.4 873,975 - +1.7 873,979 - +1.7 873,979 - -0.6 874,043 - +1.7 874,058 - +0.7 874,081 - +1.7 874,156 + nadA NIAGMN_04810 0.10 +0.2 874,157 - nadA NIAGMN_04810 0.10 +0.1 874,396 - nadA NIAGMN_04810 0.33 +0.1 874,510 + nadA NIAGMN_04810 0.44 -0.7 874,841 - nadA NIAGMN_04810 0.76 -0.6 874,899 + nadA NIAGMN_04810 0.82 -0.6 875,220 + +1.8 875,264 + -0.8 875,293 - -0.6 875,335 - +0.1 875,352 - -0.5 875,365 - -0.4 875,471 - +0.4 875,567 - +0.6 875,567 - -1.4 875,749 - +1.0 875,761 - -1.2 875,761 - +0.5 875,764 - -0.3 875,771 - +0.4 875,771 - -0.1 875,791 - -1.7 875,791 - -1.2 875,795 - +0.9 875,795 - -0.8 875,957 - +0.9 875,957 - +1.8 875,957 - +0.8 875,969 - -2.4 875,969 - +3.1 875,969 - +0.1 875,975 - -0.2 875,975 - -0.6 875,975 - -1.5 875,975 - +0.5 875,979 - -1.2 875,979 - -0.7 875,979 - +0.8 875,979 - +1.2
Or see this region's nucleotide sequence