Strain Fitness in Escherichia coli ECRC102 around NIAGMN_04685

Experiment: Bas14

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntNIAGMN_04665 and NIAGMN_04670 overlap by 4 nucleotidesNIAGMN_04670 and NIAGMN_04675 overlap by 4 nucleotidesNIAGMN_04675 and NIAGMN_04680 are separated by 98 nucleotidesNIAGMN_04680 and NIAGMN_04685 are separated by 210 nucleotidesNIAGMN_04685 and NIAGMN_04690 are separated by 242 nucleotidesNIAGMN_04690 and int are separated by 531 nucleotides NIAGMN_04660: NIAGMN_04660 - cruciferin, at 846,361 to 846,543 _04660 NIAGMN_04665: NIAGMN_04665 - DUF1382 domain-containing protein, at 846,516 to 846,707 _04665 NIAGMN_04670: NIAGMN_04670 - DUF1382 domain-containing protein, at 846,704 to 846,787 _04670 NIAGMN_04675: NIAGMN_04675 - Cell division protein ZapA, at 846,784 to 846,999 _04675 NIAGMN_04680: NIAGMN_04680 - conjugal transfer protein TraR, at 847,098 to 847,319 _04680 NIAGMN_04685: NIAGMN_04685 - hypothetical protein, at 847,530 to 848,132 _04685 NIAGMN_04690: NIAGMN_04690 - hypothetical protein, at 848,375 to 848,542 _04690 NIAGMN_04695: int - Integrase, at 849,074 to 849,844 int Position (kb) 847 848 849Strain fitness (log2 ratio) -1 0 1 2at 846.541 kb on + strandat 846.704 kb on - strandat 846.975 kb on - strand, within NIAGMN_04675at 847.561 kb on + strandat 847.578 kb on + strandat 847.618 kb on + strand, within NIAGMN_04685at 847.625 kb on - strand, within NIAGMN_04685at 847.807 kb on - strand, within NIAGMN_04685at 848.372 kb on + strandat 848.453 kb on + strand, within NIAGMN_04690at 848.453 kb on + strand, within NIAGMN_04690at 848.481 kb on + strand, within NIAGMN_04690at 848.585 kb on + strandat 848.585 kb on + strandat 848.586 kb on - strandat 848.702 kb on + strandat 848.703 kb on - strandat 848.725 kb on - strandat 848.828 kb on + strandat 848.828 kb on + strandat 848.828 kb on + strandat 848.828 kb on + strandat 848.828 kb on + strandat 848.830 kb on + strandat 848.830 kb on + strandat 848.830 kb on + strandat 848.831 kb on - strandat 848.831 kb on - strandat 848.840 kb on + strandat 848.841 kb on - strandat 848.843 kb on + strandat 848.849 kb on + strandat 848.919 kb on + strandat 848.919 kb on + strandat 848.929 kb on + strandat 848.986 kb on + strandat 849.001 kb on - strandat 849.066 kb on + strandat 849.071 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas14
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846,541 + +2.1
846,704 - +1.7
846,975 - NIAGMN_04675 0.88 +1.7
847,561 + +0.7
847,578 + -1.6
847,618 + NIAGMN_04685 0.15 +0.9
847,625 - NIAGMN_04685 0.16 +1.7
847,807 - NIAGMN_04685 0.46 +0.7
848,372 + -0.7
848,453 + NIAGMN_04690 0.46 +1.1
848,453 + NIAGMN_04690 0.46 -0.0
848,481 + NIAGMN_04690 0.63 -0.5
848,585 + +1.1
848,585 + +0.7
848,586 - +1.7
848,702 + -0.0
848,703 - +0.9
848,725 - -0.5
848,828 + -1.1
848,828 + +1.1
848,828 + +0.7
848,828 + -0.3
848,828 + +0.6
848,830 + -1.7
848,830 + -0.3
848,830 + +1.3
848,831 - +1.4
848,831 - +1.7
848,840 + +1.5
848,841 - -0.3
848,843 + -1.0
848,849 + +0.4
848,919 + -1.7
848,919 + -0.3
848,929 + +2.5
848,986 + -0.7
849,001 - -1.1
849,066 + -0.1
849,071 + -1.3

Or see this region's nucleotide sequence