Experiment: Bas14
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fliP and fliQ are separated by 9 nucleotides fliQ and fliR are separated by 7 nucleotides fliR and rcsA are separated by 289 nucleotides rcsA and dsrB are separated by 43 nucleotides
NIAGMN_02390: fliP - flagellar type III secretion system pore protein FliP, at 422,004 to 422,741
fliP
NIAGMN_02395: fliQ - flagellar biosynthesis protein FliQ, at 422,751 to 423,020
fliQ
NIAGMN_02400: fliR - flagellar type III secretion system protein FliR, at 423,028 to 423,813
fliR
NIAGMN_02405: rcsA - transcriptional regulator RcsA, at 424,103 to 424,726
rcsA
NIAGMN_02410: dsrB - protein DsrB, at 424,770 to 424,958
dsrB
Position (kb) 423
424 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 422.259 kb on - strand, within fliP at 422.267 kb on - strand, within fliP at 422.733 kb on + strand at 422.996 kb on + strand at 423.006 kb on + strand at 423.028 kb on - strand at 423.073 kb on + strand at 423.285 kb on + strand, within fliR at 423.388 kb on - strand, within fliR at 423.388 kb on - strand, within fliR at 423.458 kb on + strand, within fliR at 423.459 kb on - strand, within fliR at 423.646 kb on - strand, within fliR at 423.790 kb on - strand at 423.985 kb on + strand at 423.985 kb on + strand at 424.074 kb on + strand at 424.097 kb on + strand at 424.655 kb on - strand, within rcsA
Per-strain Table
Position Strand Gene LocusTag Fraction Bas14 remove 422,259 - fliP NIAGMN_02390 0.35 +3.3 422,267 - fliP NIAGMN_02390 0.36 -0.2 422,733 + -0.6 422,996 + +0.2 423,006 + -0.6 423,028 - -0.8 423,073 + +1.7 423,285 + fliR NIAGMN_02400 0.33 +0.4 423,388 - fliR NIAGMN_02400 0.46 +1.2 423,388 - fliR NIAGMN_02400 0.46 +1.3 423,458 + fliR NIAGMN_02400 0.55 -0.1 423,459 - fliR NIAGMN_02400 0.55 +0.3 423,646 - fliR NIAGMN_02400 0.79 +0.2 423,790 - +0.0 423,985 + +0.7 423,985 + -0.3 424,074 + +0.2 424,097 + -0.1 424,655 - rcsA NIAGMN_02405 0.88 -2.6
Or see this region's nucleotide sequence