Strain Fitness in Escherichia coli ECRC102 around NIAGMN_02220

Experiment: Bas14

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntLeu_trna and Cys_trna are separated by 12 nucleotidesCys_trna and Gly_trna are separated by 53 nucleotidesGly_trna and pgsA are separated by 151 nucleotidespgsA and uvrC are separated by 56 nucleotides NIAGMN_02205: Leu_trna - tRNA-Leu(taa), at 389,675 to 389,761 _trna NIAGMN_02210: Cys_trna - tRNA-Cys(gca), at 389,774 to 389,847 _trna NIAGMN_02215: Gly_trna - tRNA-Gly(gcc), at 389,901 to 389,976 _trna NIAGMN_02220: pgsA - CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase, at 390,128 to 390,676 pgsA NIAGMN_02225: uvrC - excinuclease ABC subunit UvrC, at 390,733 to 392,565 uvrC Position (kb) 390 391Strain fitness (log2 ratio) -1 0 1 2 3at 389.543 kb on + strandat 389.606 kb on - strandat 389.996 kb on - strandat 390.842 kb on + strandat 390.843 kb on - strandat 390.979 kb on - strand, within uvrCat 391.174 kb on - strand, within uvrCat 391.194 kb on + strand, within uvrCat 391.360 kb on - strand, within uvrC

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas14
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389,543 + -0.4
389,606 - -0.3
389,996 - +1.7
390,842 + -0.3
390,843 - +3.0
390,979 - uvrC NIAGMN_02225 0.13 +0.7
391,174 - uvrC NIAGMN_02225 0.24 +0.7
391,194 + uvrC NIAGMN_02225 0.25 +0.7
391,360 - uvrC NIAGMN_02225 0.34 +0.1

Or see this region's nucleotide sequence