Strain Fitness in Escherichia coli ECRC102 around NIAGMN_00645

Experiment: Bas14

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntNIAGMN_00635 and rnt are separated by 102 nucleotidesrnt and lhr are separated by 92 nucleotideslhr and grxD are separated by 50 nucleotidesgrxD and mepH are separated by 334 nucleotides NIAGMN_00635: NIAGMN_00635 - Lactoylglutathione lyase, at 103,134 to 103,541 _00635 NIAGMN_00640: rnt - ribonuclease T, at 103,644 to 104,291 rnt NIAGMN_00645: lhr - ATP-dependent helicase, at 104,384 to 109,000 lhr NIAGMN_00650: grxD - monothiol glutaredoxin 4, at 109,051 to 109,398 grxD NIAGMN_00655: mepH - peptidoglycan DD-endopeptidase MepH, at 109,733 to 110,548 mepH Position (kb) 104 105 106 107 108 109 110Strain fitness (log2 ratio) -2 -1 0 1 2at 104.332 kb on - strandat 104.626 kb on + strandat 105.318 kb on + strand, within lhrat 105.643 kb on - strand, within lhrat 105.712 kb on - strand, within lhrat 105.712 kb on - strand, within lhrat 105.719 kb on - strand, within lhrat 105.898 kb on + strand, within lhrat 106.046 kb on + strand, within lhrat 106.122 kb on - strand, within lhrat 106.624 kb on + strand, within lhrat 107.083 kb on - strand, within lhrat 107.469 kb on + strand, within lhrat 107.929 kb on + strand, within lhrat 108.206 kb on - strand, within lhrat 108.943 kb on + strandat 109.080 kb on - strandat 109.080 kb on - strandat 109.128 kb on - strand, within grxDat 109.201 kb on - strand, within grxDat 109.201 kb on - strand, within grxDat 109.201 kb on - strand, within grxDat 109.201 kb on - strand, within grxDat 109.206 kb on - strand, within grxDat 109.253 kb on + strand, within grxDat 109.423 kb on - strandat 109.498 kb on - strandat 109.668 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas14
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104,332 - +0.9
104,626 + +0.3
105,318 + lhr NIAGMN_00645 0.20 +1.0
105,643 - lhr NIAGMN_00645 0.27 +1.2
105,712 - lhr NIAGMN_00645 0.29 +0.1
105,712 - lhr NIAGMN_00645 0.29 -0.1
105,719 - lhr NIAGMN_00645 0.29 +2.4
105,898 + lhr NIAGMN_00645 0.33 -1.1
106,046 + lhr NIAGMN_00645 0.36 +0.1
106,122 - lhr NIAGMN_00645 0.38 +0.1
106,624 + lhr NIAGMN_00645 0.49 +0.8
107,083 - lhr NIAGMN_00645 0.58 +0.2
107,469 + lhr NIAGMN_00645 0.67 +2.1
107,929 + lhr NIAGMN_00645 0.77 -0.1
108,206 - lhr NIAGMN_00645 0.83 +1.0
108,943 + -0.7
109,080 - +0.7
109,080 - -0.7
109,128 - grxD NIAGMN_00650 0.22 +0.7
109,201 - grxD NIAGMN_00650 0.43 -1.6
109,201 - grxD NIAGMN_00650 0.43 -0.8
109,201 - grxD NIAGMN_00650 0.43 -1.1
109,201 - grxD NIAGMN_00650 0.43 -1.5
109,206 - grxD NIAGMN_00650 0.45 -1.8
109,253 + grxD NIAGMN_00650 0.58 +1.7
109,423 - +0.3
109,498 - +1.7
109,668 - +0.1

Or see this region's nucleotide sequence