Experiment: Ying_all64 rep B; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF985 and GFF986 are separated by 10 nucleotides GFF986 and GFF987 are separated by 25 nucleotides GFF987 and GFF988 overlap by 4 nucleotides
GFF985 - Methylcrotonyl-CoA carboxylase carboxyl transferase subunit (EC 6.4.1.4), at 723,667 to 725,262
GFF985
GFF986 - Methylglutaconyl-CoA hydratase (EC 4.2.1.18), at 725,273 to 726,070
GFF986
GFF987 - Methylcrotonyl-CoA carboxylase biotin-containing subunit (EC 6.4.1.4), at 726,096 to 728,120
GFF987
GFF988 - Butyryl-CoA dehydrogenase (EC 1.3.99.2), at 728,117 to 729,262
GFF988
Position (kb) 726
727
728
729 Strain fitness (log2 ratio) -2
-1
0
1 at 725.096 kb on + strand, within GFF985 at 725.107 kb on + strand at 725.131 kb on + strand at 725.132 kb on - strand at 725.132 kb on - strand at 725.252 kb on - strand at 725.359 kb on + strand, within GFF986 at 725.581 kb on + strand, within GFF986 at 725.582 kb on - strand, within GFF986 at 725.582 kb on - strand, within GFF986 at 725.679 kb on - strand, within GFF986 at 725.933 kb on + strand, within GFF986 at 725.933 kb on + strand, within GFF986 at 725.934 kb on - strand, within GFF986 at 725.934 kb on - strand, within GFF986 at 725.934 kb on - strand, within GFF986 at 725.969 kb on + strand, within GFF986 at 725.969 kb on + strand, within GFF986 at 726.102 kb on - strand at 726.102 kb on - strand at 726.273 kb on + strand at 726.274 kb on - strand at 727.233 kb on - strand, within GFF987 at 727.233 kb on - strand, within GFF987 at 727.264 kb on + strand, within GFF987 at 727.265 kb on - strand, within GFF987 at 727.399 kb on + strand, within GFF987 at 727.812 kb on - strand, within GFF987 at 727.940 kb on - strand at 727.986 kb on - strand at 728.000 kb on + strand at 728.000 kb on + strand at 728.133 kb on - strand at 728.271 kb on - strand, within GFF988 at 728.281 kb on - strand, within GFF988 at 728.285 kb on + strand, within GFF988 at 728.825 kb on + strand, within GFF988 at 728.866 kb on - strand, within GFF988 at 728.918 kb on + strand, within GFF988 at 728.919 kb on - strand, within GFF988
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_all64 rep B; time point 2 remove 725,096 + GFF985 0.90 +0.6 725,107 + +0.0 725,131 + -1.0 725,132 - +0.4 725,132 - +1.5 725,252 - -1.1 725,359 + GFF986 0.11 -0.9 725,581 + GFF986 0.39 -0.6 725,582 - GFF986 0.39 +0.0 725,582 - GFF986 0.39 -0.8 725,679 - GFF986 0.51 -0.2 725,933 + GFF986 0.83 +1.2 725,933 + GFF986 0.83 +0.2 725,934 - GFF986 0.83 -0.7 725,934 - GFF986 0.83 +0.3 725,934 - GFF986 0.83 -2.0 725,969 + GFF986 0.87 +0.1 725,969 + GFF986 0.87 -0.1 726,102 - -0.6 726,102 - -0.2 726,273 + -0.7 726,274 - +0.5 727,233 - GFF987 0.56 -0.2 727,233 - GFF987 0.56 -0.0 727,264 + GFF987 0.58 +0.7 727,265 - GFF987 0.58 +0.2 727,399 + GFF987 0.64 +0.6 727,812 - GFF987 0.85 +0.8 727,940 - -0.3 727,986 - -0.5 728,000 + -0.1 728,000 + -0.2 728,133 - -0.2 728,271 - GFF988 0.13 +0.1 728,281 - GFF988 0.14 -0.7 728,285 + GFF988 0.15 +0.6 728,825 + GFF988 0.62 +0.6 728,866 - GFF988 0.65 -0.2 728,918 + GFF988 0.70 -0.2 728,919 - GFF988 0.70 -0.4
Or see this region's nucleotide sequence