Experiment: Ying_all64 rep B; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF827 and GFF828 overlap by 4 nucleotides GFF828 and GFF829 are separated by 15 nucleotides GFF829 and GFF830 are separated by 77 nucleotides
GFF827 - A/G-specific adenine glycosylase (EC 3.2.2.-), at 522,549 to 523,643
GFF827
GFF828 - Hypothetical ATP-binding protein UPF0042, contains P-loop, at 523,640 to 524,563
GFF828
GFF829 - DNA repair protein RecN, at 524,579 to 526,201
GFF829
GFF830 - NAD kinase (EC 2.7.1.23), at 526,279 to 527,145
GFF830
Position (kb) 524
525
526
527 Strain fitness (log2 ratio) -1
0
1 at 523.641 kb on + strand at 523.641 kb on + strand at 523.641 kb on + strand at 523.642 kb on - strand at 523.642 kb on - strand at 523.642 kb on - strand at 523.642 kb on - strand at 523.800 kb on + strand, within GFF828 at 523.801 kb on - strand, within GFF828 at 523.801 kb on - strand, within GFF828 at 523.896 kb on + strand, within GFF828 at 523.897 kb on - strand, within GFF828 at 523.948 kb on - strand, within GFF828 at 524.460 kb on + strand, within GFF828 at 525.375 kb on + strand, within GFF829 at 525.376 kb on - strand, within GFF829 at 525.780 kb on + strand, within GFF829 at 525.780 kb on + strand, within GFF829 at 525.781 kb on - strand, within GFF829 at 526.263 kb on + strand at 526.264 kb on - strand at 527.197 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_all64 rep B; time point 2 remove 523,641 + -0.1 523,641 + -0.0 523,641 + +0.1 523,642 - +0.6 523,642 - +0.7 523,642 - -0.5 523,642 - +0.2 523,800 + GFF828 0.17 +0.8 523,801 - GFF828 0.17 -0.6 523,801 - GFF828 0.17 -0.1 523,896 + GFF828 0.28 +0.6 523,897 - GFF828 0.28 +0.6 523,948 - GFF828 0.33 -1.2 524,460 + GFF828 0.89 +0.2 525,375 + GFF829 0.49 -0.0 525,376 - GFF829 0.49 +1.0 525,780 + GFF829 0.74 +1.3 525,780 + GFF829 0.74 -0.1 525,781 - GFF829 0.74 -0.1 526,263 + -0.4 526,264 - +0.3 527,197 - -0.3
Or see this region's nucleotide sequence