Experiment: Ying_all64 rep B; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5108 and GFF5109 are separated by 18 nucleotides GFF5109 and GFF5110 are separated by 96 nucleotides GFF5110 and GFF5111 overlap by 4 nucleotides
GFF5108 - Outer membrane protein, at 2,120,910 to 2,121,566
GFF5108
GFF5109 - High potential iron-sulfur protein, at 2,121,585 to 2,121,896
GFF5109
GFF5110 - Phytoene synthase (EC 2.5.1.32), at 2,121,993 to 2,122,859
GFF5110
GFF5111 - Phytoene desaturase, pro-zeta-carotene producing (EC 1.-.-.-), at 2,122,856 to 2,124,223
GFF5111
Position (kb)
2121
2122
2123 Strain fitness (log2 ratio)
-2
-1
0
1 at 2121.168 kb on + strand, within GFF5108 at 2121.267 kb on + strand, within GFF5108 at 2121.268 kb on - strand, within GFF5108 at 2121.268 kb on - strand, within GFF5108 at 2121.268 kb on - strand, within GFF5108 at 2121.767 kb on - strand, within GFF5109 at 2121.965 kb on + strand at 2121.965 kb on + strand at 2121.965 kb on + strand at 2122.008 kb on + strand at 2122.008 kb on + strand at 2122.009 kb on - strand at 2122.009 kb on - strand at 2122.048 kb on - strand at 2122.048 kb on - strand at 2122.051 kb on - strand at 2122.051 kb on - strand at 2122.101 kb on + strand, within GFF5110 at 2122.204 kb on - strand, within GFF5110 at 2122.204 kb on - strand, within GFF5110 at 2122.260 kb on + strand, within GFF5110 at 2122.261 kb on - strand, within GFF5110 at 2122.261 kb on - strand, within GFF5110 at 2122.261 kb on - strand, within GFF5110 at 2122.356 kb on + strand, within GFF5110 at 2122.515 kb on + strand, within GFF5110 at 2122.515 kb on + strand, within GFF5110 at 2122.515 kb on + strand, within GFF5110 at 2122.515 kb on + strand, within GFF5110 at 2122.515 kb on + strand, within GFF5110 at 2122.516 kb on - strand, within GFF5110 at 2122.516 kb on - strand, within GFF5110 at 2122.662 kb on + strand, within GFF5110 at 2122.738 kb on - strand, within GFF5110 at 2122.738 kb on - strand, within GFF5110 at 2122.738 kb on - strand, within GFF5110 at 2122.846 kb on + strand at 2122.911 kb on + strand at 2122.912 kb on - strand at 2122.912 kb on - strand at 2123.054 kb on + strand, within GFF5111 at 2123.798 kb on + strand, within GFF5111 at 2123.799 kb on - strand, within GFF5111
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_all64 rep B; time point 2 remove 2,121,168 + GFF5108 0.39 -1.2 2,121,267 + GFF5108 0.54 +0.1 2,121,268 - GFF5108 0.54 +0.0 2,121,268 - GFF5108 0.54 -0.1 2,121,268 - GFF5108 0.54 +0.8 2,121,767 - GFF5109 0.58 +0.4 2,121,965 + +0.4 2,121,965 + -0.5 2,121,965 + -0.5 2,122,008 + -1.4 2,122,008 + +0.1 2,122,009 - -1.4 2,122,009 - -1.3 2,122,048 - -1.5 2,122,048 - -0.6 2,122,051 - -1.5 2,122,051 - -1.1 2,122,101 + GFF5110 0.12 -1.0 2,122,204 - GFF5110 0.24 -0.7 2,122,204 - GFF5110 0.24 -1.4 2,122,260 + GFF5110 0.31 -1.1 2,122,261 - GFF5110 0.31 -1.8 2,122,261 - GFF5110 0.31 -0.6 2,122,261 - GFF5110 0.31 -1.7 2,122,356 + GFF5110 0.42 -1.3 2,122,515 + GFF5110 0.60 -1.0 2,122,515 + GFF5110 0.60 -1.7 2,122,515 + GFF5110 0.60 -1.1 2,122,515 + GFF5110 0.60 -1.6 2,122,515 + GFF5110 0.60 -1.6 2,122,516 - GFF5110 0.60 -2.0 2,122,516 - GFF5110 0.60 -1.0 2,122,662 + GFF5110 0.77 -2.4 2,122,738 - GFF5110 0.86 -1.1 2,122,738 - GFF5110 0.86 -1.1 2,122,738 - GFF5110 0.86 -0.1 2,122,846 + -0.4 2,122,911 + +0.2 2,122,912 - -0.1 2,122,912 - -0.1 2,123,054 + GFF5111 0.14 -0.3 2,123,798 + GFF5111 0.69 +0.1 2,123,799 - GFF5111 0.69 +0.3
Or see this region's nucleotide sequence