Experiment: Ying_all64 rep B; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4060 and GFF4061 are separated by 43 nucleotides GFF4061 and GFF4062 overlap by 8 nucleotides GFF4062 and GFF4063 overlap by 4 nucleotides GFF4063 and GFF4064 are separated by 24 nucleotides GFF4064 and GFF4065 overlap by 4 nucleotides
GFF4060 - Flagellar hook-associated protein FliD, at 978,156 to 979,514
GFF4060
GFF4061 - Flagellar biosynthesis protein FliS, at 979,558 to 979,944
GFF4061
GFF4062 - hypothetical protein, at 979,937 to 980,248
GFF4062
GFF4063 - Flagellar hook-length control protein FliK, at 980,245 to 981,282
GFF4063
GFF4064 - hypothetical protein, at 981,307 to 981,759
GFF4064
GFF4065 - RNA polymerase sigma factor for flagellar operon, at 981,756 to 982,481
GFF4065
Position (kb)
980
981
982 Strain fitness (log2 ratio)
-1
0
1
2 at 979.293 kb on + strand, within GFF4060 at 979.294 kb on - strand, within GFF4060 at 979.294 kb on - strand, within GFF4060 at 979.294 kb on - strand, within GFF4060 at 979.416 kb on + strand at 979.417 kb on - strand at 979.425 kb on + strand at 979.426 kb on - strand at 979.564 kb on + strand at 979.564 kb on + strand at 979.564 kb on + strand at 979.565 kb on - strand at 979.565 kb on - strand at 979.582 kb on + strand at 979.816 kb on + strand, within GFF4061 at 979.817 kb on - strand, within GFF4061 at 979.823 kb on - strand, within GFF4061 at 980.096 kb on + strand, within GFF4062 at 980.210 kb on + strand, within GFF4062 at 981.383 kb on - strand, within GFF4064 at 981.451 kb on + strand, within GFF4064 at 981.514 kb on + strand, within GFF4064 at 981.514 kb on + strand, within GFF4064 at 981.515 kb on - strand, within GFF4064 at 981.682 kb on + strand, within GFF4064 at 981.772 kb on - strand at 981.772 kb on - strand at 982.180 kb on - strand, within GFF4065
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_all64 rep B; time point 2 remove 979,293 + GFF4060 0.84 -0.4 979,294 - GFF4060 0.84 +0.2 979,294 - GFF4060 0.84 +0.2 979,294 - GFF4060 0.84 -0.4 979,416 + -0.5 979,417 - +0.9 979,425 + +0.4 979,426 - +1.2 979,564 + +1.8 979,564 + -0.1 979,564 + +0.3 979,565 - +0.3 979,565 - -0.9 979,582 + +0.2 979,816 + GFF4061 0.67 -0.5 979,817 - GFF4061 0.67 -0.8 979,823 - GFF4061 0.68 -0.5 980,096 + GFF4062 0.51 +0.5 980,210 + GFF4062 0.88 -0.0 981,383 - GFF4064 0.17 +0.5 981,451 + GFF4064 0.32 +0.1 981,514 + GFF4064 0.46 +0.2 981,514 + GFF4064 0.46 +0.6 981,515 - GFF4064 0.46 +0.1 981,682 + GFF4064 0.83 -0.7 981,772 - -0.2 981,772 - +0.1 982,180 - GFF4065 0.58 -0.2
Or see this region's nucleotide sequence