Experiment: Ying_all64 rep B; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1338 and GFF1339 overlap by 11 nucleotides GFF1339 and GFF1340 overlap by 1 nucleotides GFF1340 and GFF1341 are separated by 3 nucleotides
GFF1338 - Multimodular transpeptidase-transglycosylase (EC 2.4.1.129) (EC 3.4.-.-), at 190,968 to 193,265
GFF1338
GFF1339 - Frataxin homolog CyaY, facilitates iron supply for heme A synthesis or Fe-S cluster assembly, at 193,255 to 193,584
GFF1339
GFF1340 - hypothetical protein, at 193,584 to 193,826
GFF1340
GFF1341 - Diaminopimelate decarboxylase (EC 4.1.1.20), at 193,830 to 195,119
GFF1341
Position (kb) 193
194 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 192.312 kb on + strand, within GFF1338 at 192.312 kb on + strand, within GFF1338 at 192.313 kb on - strand, within GFF1338 at 192.313 kb on - strand, within GFF1338 at 192.376 kb on - strand, within GFF1338 at 192.420 kb on + strand, within GFF1338 at 192.421 kb on - strand, within GFF1338 at 192.573 kb on + strand, within GFF1338 at 192.711 kb on + strand, within GFF1338 at 192.711 kb on + strand, within GFF1338 at 192.855 kb on + strand, within GFF1338 at 192.856 kb on - strand, within GFF1338 at 192.907 kb on - strand, within GFF1338 at 193.050 kb on + strand at 193.051 kb on - strand at 193.051 kb on - strand at 193.051 kb on - strand at 193.051 kb on - strand at 193.051 kb on - strand at 193.093 kb on - strand at 193.095 kb on + strand at 193.095 kb on + strand at 193.095 kb on + strand at 193.096 kb on - strand at 193.096 kb on - strand at 193.096 kb on - strand at 193.096 kb on - strand at 193.367 kb on + strand, within GFF1339 at 193.367 kb on + strand, within GFF1339 at 193.368 kb on - strand, within GFF1339 at 193.368 kb on - strand, within GFF1339 at 193.368 kb on - strand, within GFF1339 at 193.368 kb on - strand, within GFF1339 at 193.368 kb on - strand, within GFF1339 at 193.368 kb on - strand, within GFF1339 at 193.368 kb on - strand, within GFF1339 at 193.368 kb on - strand, within GFF1339 at 193.499 kb on + strand, within GFF1339 at 193.569 kb on - strand at 193.585 kb on + strand at 193.585 kb on + strand at 193.585 kb on + strand at 193.585 kb on + strand at 193.586 kb on - strand at 193.586 kb on - strand at 193.586 kb on - strand at 193.586 kb on - strand at 193.586 kb on - strand at 193.619 kb on + strand, within GFF1340 at 193.619 kb on + strand, within GFF1340 at 193.619 kb on + strand, within GFF1340 at 193.619 kb on + strand, within GFF1340 at 193.619 kb on + strand, within GFF1340 at 193.620 kb on - strand, within GFF1340 at 193.620 kb on - strand, within GFF1340 at 193.620 kb on - strand, within GFF1340 at 193.620 kb on - strand, within GFF1340 at 193.620 kb on - strand, within GFF1340 at 193.620 kb on - strand, within GFF1340 at 193.620 kb on - strand, within GFF1340 at 193.705 kb on + strand, within GFF1340 at 193.705 kb on + strand, within GFF1340 at 193.706 kb on - strand, within GFF1340 at 193.706 kb on - strand, within GFF1340 at 193.706 kb on - strand, within GFF1340 at 193.959 kb on + strand, within GFF1341 at 194.034 kb on + strand, within GFF1341 at 194.346 kb on + strand, within GFF1341 at 194.347 kb on - strand, within GFF1341 at 194.347 kb on - strand, within GFF1341 at 194.409 kb on + strand, within GFF1341
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_all64 rep B; time point 2 remove 192,312 + GFF1338 0.58 +0.6 192,312 + GFF1338 0.58 -0.2 192,313 - GFF1338 0.59 -1.9 192,313 - GFF1338 0.59 -0.4 192,376 - GFF1338 0.61 -0.0 192,420 + GFF1338 0.63 -0.6 192,421 - GFF1338 0.63 -0.5 192,573 + GFF1338 0.70 -1.3 192,711 + GFF1338 0.76 -0.2 192,711 + GFF1338 0.76 -0.6 192,855 + GFF1338 0.82 +0.6 192,856 - GFF1338 0.82 +0.3 192,907 - GFF1338 0.84 -1.0 193,050 + +0.2 193,051 - +1.6 193,051 - +0.6 193,051 - +0.1 193,051 - -0.6 193,051 - +0.2 193,093 - -0.7 193,095 + -1.7 193,095 + -0.3 193,095 + -0.9 193,096 - -0.4 193,096 - -0.7 193,096 - -0.2 193,096 - -0.3 193,367 + GFF1339 0.34 -1.9 193,367 + GFF1339 0.34 -0.5 193,368 - GFF1339 0.34 -2.2 193,368 - GFF1339 0.34 -1.4 193,368 - GFF1339 0.34 -1.3 193,368 - GFF1339 0.34 -1.4 193,368 - GFF1339 0.34 -1.0 193,368 - GFF1339 0.34 -1.9 193,368 - GFF1339 0.34 -1.3 193,368 - GFF1339 0.34 -0.4 193,499 + GFF1339 0.74 -1.3 193,569 - -2.1 193,585 + -0.1 193,585 + -0.2 193,585 + -0.2 193,585 + -0.4 193,586 - -0.6 193,586 - -0.0 193,586 - -0.3 193,586 - -0.3 193,586 - -1.1 193,619 + GFF1340 0.14 +0.1 193,619 + GFF1340 0.14 -4.0 193,619 + GFF1340 0.14 -2.0 193,619 + GFF1340 0.14 +0.6 193,619 + GFF1340 0.14 -0.4 193,620 - GFF1340 0.15 +0.5 193,620 - GFF1340 0.15 -0.2 193,620 - GFF1340 0.15 -0.1 193,620 - GFF1340 0.15 +0.1 193,620 - GFF1340 0.15 -0.1 193,620 - GFF1340 0.15 -1.3 193,620 - GFF1340 0.15 -0.6 193,705 + GFF1340 0.50 -3.3 193,705 + GFF1340 0.50 -3.3 193,706 - GFF1340 0.50 -2.9 193,706 - GFF1340 0.50 -1.0 193,706 - GFF1340 0.50 -4.5 193,959 + GFF1341 0.10 +0.7 194,034 + GFF1341 0.16 -1.2 194,346 + GFF1341 0.40 -3.2 194,347 - GFF1341 0.40 -1.6 194,347 - GFF1341 0.40 -1.4 194,409 + GFF1341 0.45 -3.4
Or see this region's nucleotide sequence