Experiment: Supernatant; Pseudomonas DC3000 grown in LB; 0.1 X
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt murI and COO64_RS03250 are separated by 116 nucleotides COO64_RS03250 and COO64_RS03255 are separated by 24 nucleotides COO64_RS03255 and phrB overlap by 4 nucleotides
COO64_RS03245: murI - glutamate racemase, at 126,636 to 127,436
murI
COO64_RS03250: COO64_RS03250 - acyloxyacyl hydrolase, at 127,553 to 128,071
_RS03250
COO64_RS03255: COO64_RS03255 - SDR family NAD(P)-dependent oxidoreductase, at 128,096 to 128,818
_RS03255
COO64_RS03260: phrB - deoxyribodipyrimidine photo-lyase, at 128,815 to 130,257
phrB
Position (kb) 128
129 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 127.403 kb on + strand at 127.404 kb on - strand at 127.404 kb on - strand at 127.435 kb on - strand at 127.471 kb on + strand at 127.471 kb on + strand at 127.498 kb on - strand at 127.533 kb on + strand at 127.533 kb on + strand at 127.533 kb on + strand at 127.533 kb on + strand at 127.534 kb on - strand at 127.534 kb on - strand at 127.609 kb on + strand, within COO64_RS03250 at 127.609 kb on + strand, within COO64_RS03250 at 127.629 kb on + strand, within COO64_RS03250 at 127.629 kb on + strand, within COO64_RS03250 at 127.629 kb on + strand, within COO64_RS03250 at 127.630 kb on - strand, within COO64_RS03250 at 127.630 kb on - strand, within COO64_RS03250 at 127.630 kb on - strand, within COO64_RS03250 at 127.630 kb on - strand, within COO64_RS03250 at 127.630 kb on - strand, within COO64_RS03250 at 127.671 kb on - strand, within COO64_RS03250 at 127.671 kb on - strand, within COO64_RS03250 at 127.671 kb on - strand, within COO64_RS03250 at 127.717 kb on + strand, within COO64_RS03250 at 127.717 kb on + strand, within COO64_RS03250 at 127.717 kb on + strand, within COO64_RS03250 at 127.718 kb on - strand, within COO64_RS03250 at 127.718 kb on - strand, within COO64_RS03250 at 127.718 kb on - strand, within COO64_RS03250 at 127.718 kb on - strand, within COO64_RS03250 at 127.722 kb on + strand, within COO64_RS03250 at 127.722 kb on + strand, within COO64_RS03250 at 127.754 kb on + strand, within COO64_RS03250 at 127.754 kb on + strand, within COO64_RS03250 at 127.754 kb on + strand, within COO64_RS03250 at 127.754 kb on + strand, within COO64_RS03250 at 127.754 kb on + strand, within COO64_RS03250 at 127.754 kb on + strand, within COO64_RS03250 at 127.754 kb on + strand, within COO64_RS03250 at 127.754 kb on + strand, within COO64_RS03250 at 127.755 kb on - strand, within COO64_RS03250 at 127.755 kb on - strand, within COO64_RS03250 at 127.755 kb on - strand, within COO64_RS03250 at 127.755 kb on - strand, within COO64_RS03250 at 127.760 kb on + strand, within COO64_RS03250 at 127.760 kb on + strand, within COO64_RS03250 at 127.761 kb on - strand, within COO64_RS03250 at 127.761 kb on - strand, within COO64_RS03250 at 127.761 kb on - strand, within COO64_RS03250 at 127.761 kb on - strand, within COO64_RS03250 at 127.850 kb on + strand, within COO64_RS03250 at 127.850 kb on + strand, within COO64_RS03250 at 127.851 kb on - strand, within COO64_RS03250 at 127.851 kb on - strand, within COO64_RS03250 at 127.904 kb on - strand, within COO64_RS03250 at 127.977 kb on + strand, within COO64_RS03250 at 127.977 kb on + strand, within COO64_RS03250 at 127.977 kb on + strand, within COO64_RS03250 at 127.977 kb on + strand, within COO64_RS03250 at 127.977 kb on + strand, within COO64_RS03250 at 127.978 kb on - strand, within COO64_RS03250 at 127.978 kb on - strand, within COO64_RS03250 at 127.978 kb on - strand, within COO64_RS03250 at 127.978 kb on - strand, within COO64_RS03250 at 127.978 kb on - strand, within COO64_RS03250 at 127.983 kb on + strand, within COO64_RS03250 at 127.983 kb on + strand, within COO64_RS03250 at 127.983 kb on + strand, within COO64_RS03250 at 127.984 kb on - strand, within COO64_RS03250 at 127.984 kb on - strand, within COO64_RS03250 at 127.997 kb on + strand, within COO64_RS03250 at 128.054 kb on + strand at 128.054 kb on + strand at 128.055 kb on - strand at 128.059 kb on + strand at 128.059 kb on + strand at 128.060 kb on - strand at 128.060 kb on - strand at 128.060 kb on - strand at 128.060 kb on - strand at 128.060 kb on - strand at 128.097 kb on + strand at 128.097 kb on + strand at 128.098 kb on - strand at 128.098 kb on - strand at 128.296 kb on + strand, within COO64_RS03255 at 128.397 kb on + strand, within COO64_RS03255 at 128.541 kb on + strand, within COO64_RS03255 at 128.542 kb on - strand, within COO64_RS03255 at 128.654 kb on + strand, within COO64_RS03255 at 128.654 kb on + strand, within COO64_RS03255 at 128.654 kb on + strand, within COO64_RS03255 at 128.655 kb on - strand, within COO64_RS03255 at 128.655 kb on - strand, within COO64_RS03255 at 128.690 kb on + strand, within COO64_RS03255 at 128.690 kb on + strand, within COO64_RS03255 at 128.690 kb on + strand, within COO64_RS03255 at 128.690 kb on + strand, within COO64_RS03255 at 128.690 kb on + strand, within COO64_RS03255 at 128.690 kb on + strand, within COO64_RS03255 at 128.690 kb on + strand, within COO64_RS03255 at 128.691 kb on - strand, within COO64_RS03255 at 128.691 kb on - strand, within COO64_RS03255 at 128.822 kb on - strand at 128.901 kb on + strand at 128.901 kb on + strand at 128.901 kb on + strand at 128.901 kb on + strand at 128.902 kb on - strand at 128.902 kb on - strand at 128.902 kb on - strand at 128.902 kb on - strand at 128.902 kb on - strand at 128.919 kb on + strand at 128.919 kb on + strand at 128.920 kb on - strand at 128.920 kb on - strand at 128.920 kb on - strand at 128.920 kb on - strand at 128.920 kb on - strand at 128.920 kb on - strand at 128.923 kb on - strand at 128.923 kb on - strand at 129.063 kb on - strand, within phrB at 129.404 kb on + strand, within phrB at 129.404 kb on + strand, within phrB at 129.404 kb on + strand, within phrB at 129.533 kb on + strand, within phrB at 129.533 kb on + strand, within phrB at 129.533 kb on + strand, within phrB at 129.534 kb on - strand, within phrB at 129.587 kb on + strand, within phrB at 129.587 kb on + strand, within phrB at 129.587 kb on + strand, within phrB at 129.587 kb on + strand, within phrB at 129.588 kb on - strand, within phrB at 129.664 kb on + strand, within phrB at 129.773 kb on - strand, within phrB at 129.773 kb on - strand, within phrB at 129.788 kb on + strand, within phrB at 129.788 kb on + strand, within phrB at 129.788 kb on + strand, within phrB at 129.788 kb on + strand, within phrB at 129.788 kb on + strand, within phrB
Per-strain Table
Position Strand Gene LocusTag Fraction Supernatant; Pseudomonas DC3000 grown in LB; 0.1 X remove 127,403 + +0.4 127,404 - -0.2 127,404 - -1.6 127,435 - +0.4 127,471 + +0.5 127,471 + -0.3 127,498 - -0.0 127,533 + +1.1 127,533 + +0.9 127,533 + +0.5 127,533 + -0.4 127,534 - -1.4 127,534 - -0.1 127,609 + COO64_RS03250 0.11 -0.5 127,609 + COO64_RS03250 0.11 -1.0 127,629 + COO64_RS03250 0.15 -0.5 127,629 + COO64_RS03250 0.15 -1.2 127,629 + COO64_RS03250 0.15 -1.9 127,630 - COO64_RS03250 0.15 -0.4 127,630 - COO64_RS03250 0.15 -1.4 127,630 - COO64_RS03250 0.15 -2.0 127,630 - COO64_RS03250 0.15 -0.2 127,630 - COO64_RS03250 0.15 -1.2 127,671 - COO64_RS03250 0.23 +0.7 127,671 - COO64_RS03250 0.23 -1.3 127,671 - COO64_RS03250 0.23 -0.6 127,717 + COO64_RS03250 0.32 -0.2 127,717 + COO64_RS03250 0.32 -0.4 127,717 + COO64_RS03250 0.32 -0.6 127,718 - COO64_RS03250 0.32 +0.0 127,718 - COO64_RS03250 0.32 +0.3 127,718 - COO64_RS03250 0.32 -0.0 127,718 - COO64_RS03250 0.32 -0.8 127,722 + COO64_RS03250 0.33 +0.6 127,722 + COO64_RS03250 0.33 -0.6 127,754 + COO64_RS03250 0.39 +0.3 127,754 + COO64_RS03250 0.39 -0.6 127,754 + COO64_RS03250 0.39 +0.8 127,754 + COO64_RS03250 0.39 +0.6 127,754 + COO64_RS03250 0.39 -0.3 127,754 + COO64_RS03250 0.39 +0.8 127,754 + COO64_RS03250 0.39 +1.4 127,754 + COO64_RS03250 0.39 -1.1 127,755 - COO64_RS03250 0.39 -0.4 127,755 - COO64_RS03250 0.39 +1.4 127,755 - COO64_RS03250 0.39 -1.2 127,755 - COO64_RS03250 0.39 +0.1 127,760 + COO64_RS03250 0.40 -0.4 127,760 + COO64_RS03250 0.40 +0.9 127,761 - COO64_RS03250 0.40 -0.1 127,761 - COO64_RS03250 0.40 -0.7 127,761 - COO64_RS03250 0.40 +0.5 127,761 - COO64_RS03250 0.40 -1.1 127,850 + COO64_RS03250 0.57 -1.5 127,850 + COO64_RS03250 0.57 -0.3 127,851 - COO64_RS03250 0.57 +0.0 127,851 - COO64_RS03250 0.57 -0.6 127,904 - COO64_RS03250 0.68 +0.1 127,977 + COO64_RS03250 0.82 -0.4 127,977 + COO64_RS03250 0.82 +0.2 127,977 + COO64_RS03250 0.82 -0.3 127,977 + COO64_RS03250 0.82 -0.4 127,977 + COO64_RS03250 0.82 +0.2 127,978 - COO64_RS03250 0.82 -1.5 127,978 - COO64_RS03250 0.82 +0.0 127,978 - COO64_RS03250 0.82 +0.6 127,978 - COO64_RS03250 0.82 -0.1 127,978 - COO64_RS03250 0.82 +0.3 127,983 + COO64_RS03250 0.83 -0.9 127,983 + COO64_RS03250 0.83 -0.5 127,983 + COO64_RS03250 0.83 -0.1 127,984 - COO64_RS03250 0.83 -0.3 127,984 - COO64_RS03250 0.83 +1.0 127,997 + COO64_RS03250 0.86 +0.2 128,054 + -0.5 128,054 + -2.0 128,055 - -0.6 128,059 + -1.3 128,059 + +0.3 128,060 - +1.4 128,060 - -0.4 128,060 - +4.3 128,060 - -0.3 128,060 - +0.4 128,097 + -0.5 128,097 + -0.2 128,098 - +0.0 128,098 - +0.8 128,296 + COO64_RS03255 0.28 -1.2 128,397 + COO64_RS03255 0.42 -0.3 128,541 + COO64_RS03255 0.62 -0.9 128,542 - COO64_RS03255 0.62 +0.2 128,654 + COO64_RS03255 0.77 +0.1 128,654 + COO64_RS03255 0.77 -0.5 128,654 + COO64_RS03255 0.77 +0.4 128,655 - COO64_RS03255 0.77 -1.0 128,655 - COO64_RS03255 0.77 -0.2 128,690 + COO64_RS03255 0.82 +0.2 128,690 + COO64_RS03255 0.82 -0.7 128,690 + COO64_RS03255 0.82 -0.4 128,690 + COO64_RS03255 0.82 -0.1 128,690 + COO64_RS03255 0.82 -0.8 128,690 + COO64_RS03255 0.82 -1.4 128,690 + COO64_RS03255 0.82 +0.2 128,691 - COO64_RS03255 0.82 +0.3 128,691 - COO64_RS03255 0.82 +0.3 128,822 - +0.3 128,901 + -0.2 128,901 + +0.1 128,901 + +0.2 128,901 + +0.3 128,902 - -2.2 128,902 - +0.4 128,902 - +0.5 128,902 - -2.4 128,902 - -0.2 128,919 + +0.0 128,919 + +0.2 128,920 - -0.3 128,920 - -0.6 128,920 - -0.3 128,920 - +0.4 128,920 - -1.0 128,920 - +0.5 128,923 - -0.8 128,923 - -0.2 129,063 - phrB COO64_RS03260 0.17 -0.6 129,404 + phrB COO64_RS03260 0.41 -0.3 129,404 + phrB COO64_RS03260 0.41 -0.6 129,404 + phrB COO64_RS03260 0.41 +0.2 129,533 + phrB COO64_RS03260 0.50 -0.5 129,533 + phrB COO64_RS03260 0.50 -1.2 129,533 + phrB COO64_RS03260 0.50 +0.0 129,534 - phrB COO64_RS03260 0.50 -0.7 129,587 + phrB COO64_RS03260 0.53 +1.3 129,587 + phrB COO64_RS03260 0.53 -0.8 129,587 + phrB COO64_RS03260 0.53 -0.8 129,587 + phrB COO64_RS03260 0.53 -2.0 129,588 - phrB COO64_RS03260 0.54 -0.2 129,664 + phrB COO64_RS03260 0.59 -0.1 129,773 - phrB COO64_RS03260 0.66 -0.2 129,773 - phrB COO64_RS03260 0.66 -1.2 129,788 + phrB COO64_RS03260 0.67 -0.5 129,788 + phrB COO64_RS03260 0.67 +0.7 129,788 + phrB COO64_RS03260 0.67 +0.6 129,788 + phrB COO64_RS03260 0.67 -1.1 129,788 + phrB COO64_RS03260 0.67 -1.9
Or see this region's nucleotide sequence