Experiment: Supernatant; Pseudomonas DC3000 grown in LB; 0.1 X
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rfbB and rfbA overlap by 4 nucleotides rfbA and rfbC overlap by 4 nucleotides rfbC and rfbD overlap by 4 nucleotides
COO64_RS00570: rfbB - dTDP-glucose 4,6-dehydratase, at 124,322 to 125,404
rfbB
COO64_RS00575: rfbA - glucose-1-phosphate thymidylyltransferase RfbA, at 125,401 to 126,276
rfbA
COO64_RS00580: rfbC - dTDP-4-dehydrorhamnose 3,5-epimerase, at 126,273 to 126,818
rfbC
COO64_RS00585: rfbD - dTDP-4-dehydrorhamnose reductase, at 126,815 to 127,687
rfbD
Position (kb) 125
126
127 Strain fitness (log2 ratio) -2
-1
0
1 at 125.301 kb on + strand at 125.301 kb on + strand at 125.301 kb on + strand at 125.354 kb on + strand at 125.354 kb on + strand at 125.354 kb on + strand at 125.354 kb on + strand at 125.354 kb on + strand at 125.354 kb on + strand at 125.354 kb on + strand at 125.354 kb on + strand at 125.355 kb on - strand at 125.355 kb on - strand at 125.451 kb on + strand at 125.477 kb on + strand at 125.632 kb on + strand, within rfbA at 125.730 kb on + strand, within rfbA at 125.798 kb on + strand, within rfbA at 125.929 kb on + strand, within rfbA at 125.929 kb on + strand, within rfbA at 125.929 kb on + strand, within rfbA at 125.929 kb on + strand, within rfbA at 126.013 kb on + strand, within rfbA at 126.013 kb on + strand, within rfbA at 126.109 kb on + strand, within rfbA at 126.244 kb on + strand at 126.244 kb on + strand at 126.244 kb on + strand at 126.244 kb on + strand at 126.245 kb on - strand at 126.245 kb on - strand at 126.245 kb on - strand at 126.245 kb on - strand at 126.245 kb on - strand at 126.245 kb on - strand at 126.245 kb on - strand at 126.280 kb on + strand at 126.280 kb on + strand at 126.280 kb on + strand at 126.280 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Supernatant; Pseudomonas DC3000 grown in LB; 0.1 X remove 125,301 + -0.0 125,301 + -0.6 125,301 + +0.3 125,354 + +0.8 125,354 + +0.3 125,354 + +0.6 125,354 + -2.7 125,354 + +1.1 125,354 + -0.1 125,354 + +0.0 125,354 + +1.4 125,355 - -0.2 125,355 - +0.2 125,451 + -0.6 125,477 + +0.7 125,632 + rfbA COO64_RS00575 0.26 -0.3 125,730 + rfbA COO64_RS00575 0.38 -0.8 125,798 + rfbA COO64_RS00575 0.45 -1.4 125,929 + rfbA COO64_RS00575 0.60 +0.6 125,929 + rfbA COO64_RS00575 0.60 +1.2 125,929 + rfbA COO64_RS00575 0.60 +0.2 125,929 + rfbA COO64_RS00575 0.60 +1.4 126,013 + rfbA COO64_RS00575 0.70 -1.6 126,013 + rfbA COO64_RS00575 0.70 +1.0 126,109 + rfbA COO64_RS00575 0.81 +0.0 126,244 + -0.2 126,244 + -0.9 126,244 + +0.3 126,244 + +0.7 126,245 - -0.6 126,245 - -0.6 126,245 - +0.0 126,245 - -0.6 126,245 - -0.1 126,245 - +0.7 126,245 - +0.5 126,280 + +0.3 126,280 + +0.0 126,280 + +0.4 126,280 + +0.4
Or see this region's nucleotide sequence