Strain Fitness in Escherichia coli ECRC102 around NIAGMN_27575

Experiment: Bas14

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntkch and clsA are separated by 370 nucleotidesclsA and cls are separated by 34 nucleotidescls and oppF are separated by 52 nucleotides NIAGMN_27570: kch - voltage-gated potassium channel protein, at 5,340,677 to 5,341,930 kch NIAGMN_27575: clsA - cardiolipin synthase ClsA, at 5,342,301 to 5,343,761 clsA NIAGMN_27580: cls - cardiolipin synthase, at 5,343,796 to 5,344,125 cls NIAGMN_27585: oppF - murein tripeptide/oligopeptide ABC transporter ATP-binding protein OppF, at 5,344,178 to 5,345,182 oppF Position (kb) 5342 5343 5344Strain fitness (log2 ratio) -2 -1 0 1 2at 5341.325 kb on - strand, within kchat 5341.981 kb on - strandat 5341.991 kb on - strandat 5341.995 kb on + strandat 5342.165 kb on + strandat 5342.171 kb on - strandat 5342.192 kb on + strandat 5342.315 kb on + strandat 5342.456 kb on + strand, within clsAat 5342.565 kb on - strand, within clsAat 5343.188 kb on + strand, within clsAat 5343.189 kb on - strand, within clsAat 5343.660 kb on - strandat 5344.405 kb on - strand, within oppFat 5344.437 kb on + strand, within oppFat 5344.438 kb on - strand, within oppFat 5344.468 kb on - strand, within oppFat 5344.468 kb on - strand, within oppFat 5344.530 kb on - strand, within oppF

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas14
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5,341,325 - kch NIAGMN_27570 0.52 +0.9
5,341,981 - +0.4
5,341,991 - +0.9
5,341,995 + -0.5
5,342,165 + -0.1
5,342,171 - -2.0
5,342,192 + +0.0
5,342,315 + -0.7
5,342,456 + clsA NIAGMN_27575 0.11 +2.3
5,342,565 - clsA NIAGMN_27575 0.18 +1.6
5,343,188 + clsA NIAGMN_27575 0.61 +0.6
5,343,189 - clsA NIAGMN_27575 0.61 -1.4
5,343,660 - -0.6
5,344,405 - oppF NIAGMN_27585 0.23 +1.6
5,344,437 + oppF NIAGMN_27585 0.26 +0.3
5,344,438 - oppF NIAGMN_27585 0.26 -1.2
5,344,468 - oppF NIAGMN_27585 0.29 -2.5
5,344,468 - oppF NIAGMN_27585 0.29 -0.7
5,344,530 - oppF NIAGMN_27585 0.35 -0.4

Or see this region's nucleotide sequence