Strain Fitness in Escherichia coli ECRC102 around NIAGMN_27535

Experiment: Bas14

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntdnaQ and NIAGMN_27535 are separated by 290 nucleotidesNIAGMN_27535 and ompW are separated by 45 nucleotides NIAGMN_27530: dnaQ - exonuclease, at 5,332,349 to 5,334,820 dnaQ NIAGMN_27535: NIAGMN_27535 - integrase, at 5,335,111 to 5,336,241 _27535 NIAGMN_27540: ompW - outer membrane protein OmpW, at 5,336,287 to 5,336,925 ompW Position (kb) 5335 5336 5337Strain fitness (log2 ratio) -1 0 1at 5334.434 kb on - strand, within dnaQat 5334.954 kb on - strandat 5336.439 kb on - strand, within ompWat 5336.730 kb on - strand, within ompWat 5336.819 kb on - strand, within ompWat 5336.993 kb on - strandat 5337.007 kb on - strandat 5337.016 kb on - strandat 5337.028 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas14
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5,334,434 - dnaQ NIAGMN_27530 0.84 +1.0
5,334,954 - -1.5
5,336,439 - ompW NIAGMN_27540 0.24 +0.8
5,336,730 - ompW NIAGMN_27540 0.69 -1.0
5,336,819 - ompW NIAGMN_27540 0.83 -0.6
5,336,993 - +0.6
5,337,007 - +0.1
5,337,016 - +1.4
5,337,028 - +0.4

Or see this region's nucleotide sequence