Experiment: Bas14
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yciH and pyrF overlap by 1 nucleotides pyrF and lapB are separated by 132 nucleotides lapB and lapA are separated by 6 nucleotides lapA and pgpB are separated by 148 nucleotides
NIAGMN_26715: yciH - stress response translation initiation inhibitor YciH, at 5,202,821 to 5,203,147
yciH
NIAGMN_26720: pyrF - orotidine-5'-phosphate decarboxylase, at 5,203,147 to 5,203,944
pyrF
NIAGMN_26725: lapB - lipopolysaccharide assembly protein LapB, at 5,204,077 to 5,205,246
lapB
NIAGMN_26730: lapA - lipopolysaccharide assembly protein LapA, at 5,205,253 to 5,205,561
lapA
NIAGMN_26735: pgpB - phosphatidylglycerophosphatase B, at 5,205,710 to 5,206,474
pgpB
Position (kb) 5204
5205
5206 Strain fitness (log2 ratio) -2
-1
0
1 at 5203.119 kb on + strand at 5203.120 kb on - strand at 5203.233 kb on + strand, within pyrF at 5203.234 kb on - strand, within pyrF at 5203.235 kb on + strand, within pyrF at 5203.734 kb on + strand, within pyrF at 5203.877 kb on - strand at 5203.947 kb on + strand at 5204.033 kb on + strand at 5205.616 kb on - strand at 5205.629 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bas14 remove 5,203,119 + +1.6 5,203,120 - +0.0 5,203,233 + pyrF NIAGMN_26720 0.11 +0.0 5,203,234 - pyrF NIAGMN_26720 0.11 -1.7 5,203,235 + pyrF NIAGMN_26720 0.11 +1.0 5,203,734 + pyrF NIAGMN_26720 0.74 +0.2 5,203,877 - +1.0 5,203,947 + -0.2 5,204,033 + -2.4 5,205,616 - +0.6 5,205,629 - +0.6
Or see this region's nucleotide sequence