Experiment: Bas14
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pdeR and yciZ are separated by 147 nucleotides yciZ and yciT are separated by 89 nucleotides yciT and osmB are separated by 268 nucleotides osmB and yciH are separated by 125 nucleotides yciH and pyrF overlap by 1 nucleotides
NIAGMN_26695: pdeR - cyclic di-GMP phosphodiesterase, at 5,199,063 to 5,201,048
pdeR
NIAGMN_26700: yciZ - UPF0509 protein YciZ, at 5,201,196 to 5,201,369
yciZ
NIAGMN_26705: yciT - DNA-binding transcriptional regulator YciT, at 5,201,459 to 5,202,208
yciT
NIAGMN_26710: osmB - osmotically-inducible lipoprotein OsmB, at 5,202,477 to 5,202,695
osmB
NIAGMN_26715: yciH - stress response translation initiation inhibitor YciH, at 5,202,821 to 5,203,147
yciH
NIAGMN_26720: pyrF - orotidine-5'-phosphate decarboxylase, at 5,203,147 to 5,203,944
pyrF
Position (kb)
5201
5202
5203 Strain fitness (log2 ratio)
-1
0
1
2 at 5200.758 kb on + strand, within pdeR at 5201.097 kb on - strand at 5201.182 kb on + strand at 5201.246 kb on - strand, within yciZ at 5201.300 kb on + strand, within yciZ at 5201.632 kb on + strand, within yciT at 5201.668 kb on + strand, within yciT at 5202.431 kb on + strand at 5202.432 kb on - strand at 5202.803 kb on + strand at 5203.119 kb on + strand at 5203.120 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bas14 remove 5,200,758 + pdeR NIAGMN_26695 0.85 +1.9 5,201,097 - -0.7 5,201,182 + -0.2 5,201,246 - yciZ NIAGMN_26700 0.29 +1.3 5,201,300 + yciZ NIAGMN_26700 0.60 +0.5 5,201,632 + yciT NIAGMN_26705 0.23 -1.5 5,201,668 + yciT NIAGMN_26705 0.28 -0.7 5,202,431 + -0.2 5,202,432 - -0.7 5,202,803 + +0.6 5,203,119 + +1.6 5,203,120 - +0.0
Or see this region's nucleotide sequence