Strain Fitness in Escherichia coli ECRC102 around NIAGMN_25285

Experiment: Bas14

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyneE and tam are separated by 3 nucleotidestam and lsrG are separated by 56 nucleotideslsrG and lsrF are separated by 23 nucleotides NIAGMN_25280: yneE - UPF0187 protein YneE, at 4,912,957 to 4,913,871 yneE NIAGMN_25285: tam - trans-aconitate 2-methyltransferase, at 4,913,875 to 4,914,633 tam NIAGMN_25290: lsrG - (4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione isomerase, at 4,914,690 to 4,914,980 lsrG NIAGMN_25295: lsrF - 3-hydroxy-5-phosphonooxypentane-2,4-dione thiolase, at 4,915,004 to 4,915,879 lsrF Position (kb) 4913 4914 4915Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 4912.914 kb on - strandat 4913.039 kb on + strandat 4913.042 kb on + strandat 4913.172 kb on + strand, within yneEat 4913.172 kb on + strand, within yneEat 4914.278 kb on - strand, within tamat 4914.611 kb on - strandat 4914.769 kb on + strand, within lsrGat 4914.814 kb on + strand, within lsrGat 4914.892 kb on - strand, within lsrGat 4914.981 kb on - strandat 4915.264 kb on - strand, within lsrFat 4915.626 kb on - strand, within lsrFat 4915.626 kb on - strand, within lsrF

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas14
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4,912,914 - -1.3
4,913,039 + -0.6
4,913,042 + +0.3
4,913,172 + yneE NIAGMN_25280 0.23 -2.8
4,913,172 + yneE NIAGMN_25280 0.23 -0.8
4,914,278 - tam NIAGMN_25285 0.53 +1.1
4,914,611 - +0.2
4,914,769 + lsrG NIAGMN_25290 0.27 +0.2
4,914,814 + lsrG NIAGMN_25290 0.43 +0.0
4,914,892 - lsrG NIAGMN_25290 0.69 +2.1
4,914,981 - -0.6
4,915,264 - lsrF NIAGMN_25295 0.30 +0.8
4,915,626 - lsrF NIAGMN_25295 0.71 -1.6
4,915,626 - lsrF NIAGMN_25295 0.71 +1.3

Or see this region's nucleotide sequence