Experiment: Bas14
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt NIAGMN_25035 and NIAGMN_25040 are separated by 41 nucleotides NIAGMN_25040 and NIAGMN_25045 are separated by 11 nucleotides NIAGMN_25045 and NIAGMN_25050 are separated by 14 nucleotides NIAGMN_25050 and NIAGMN_25055 overlap by 4 nucleotides NIAGMN_25055 and yjdB are separated by 7 nucleotides
NIAGMN_25035: NIAGMN_25035 - Major capsid protein, at 4,869,772 to 4,870,797
_25035
NIAGMN_25040: NIAGMN_25040 - DNA-packaging protein FI, at 4,870,839 to 4,871,237
_25040
NIAGMN_25045: NIAGMN_25045 - phage tail protein, at 4,871,249 to 4,871,602
_25045
NIAGMN_25050: NIAGMN_25050 - phage tail protein, at 4,871,617 to 4,872,150
_25050
NIAGMN_25055: NIAGMN_25055 - phage tail protein, at 4,872,147 to 4,872,542
_25055
NIAGMN_25060: yjdB - phage tail protein, at 4,872,550 to 4,873,302
yjdB
Position (kb) 4871
4872
4873 Strain fitness (log2 ratio) -1
0
1 at 4871.365 kb on - strand, within NIAGMN_25045 at 4871.911 kb on - strand, within NIAGMN_25050 at 4872.129 kb on + strand at 4872.155 kb on + strand at 4872.288 kb on + strand, within NIAGMN_25055 at 4872.335 kb on + strand, within NIAGMN_25055 at 4872.335 kb on + strand, within NIAGMN_25055 at 4872.335 kb on + strand, within NIAGMN_25055 at 4872.349 kb on + strand, within NIAGMN_25055 at 4872.488 kb on + strand, within NIAGMN_25055
Per-strain Table
Position Strand Gene LocusTag Fraction Bas14 remove 4,871,365 - NIAGMN_25045 0.33 -0.3 4,871,911 - NIAGMN_25050 0.55 +0.4 4,872,129 + +1.2 4,872,155 + +1.1 4,872,288 + NIAGMN_25055 0.36 -0.3 4,872,335 + NIAGMN_25055 0.47 -1.7 4,872,335 + NIAGMN_25055 0.47 -1.3 4,872,335 + NIAGMN_25055 0.47 +0.9 4,872,349 + NIAGMN_25055 0.51 -0.6 4,872,488 + NIAGMN_25055 0.86 +0.5
Or see this region's nucleotide sequence