Experiment: Bas14
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt tnp-IS3 and NIAGMN_24730 are separated by 10 nucleotides NIAGMN_24730 and NIAGMN_24735 are separated by 141 nucleotides NIAGMN_24735 and espK are separated by 188 nucleotides espK and potB are separated by 363 nucleotides
NIAGMN_24725: tnp-IS3 - IS3 family transposase, at 4,820,860 to 4,821,021
tnp-IS3
NIAGMN_24730: NIAGMN_24730 - Mutator family transposase, at 4,821,032 to 4,821,331
_24730
NIAGMN_24735: NIAGMN_24735 - hypothetical protein, at 4,821,473 to 4,821,706
_24735
NIAGMN_24740: espK - type III secretion system effector EspK, at 4,821,895 to 4,823,256
espK
NIAGMN_24745: potB - spermidine/putrescine ABC transporter permease PotB, at 4,823,620 to 4,824,483
potB
Position (kb) 4821
4822
4823
4824 Strain fitness (log2 ratio) -2
-1
0
1 at 4821.037 kb on + strand at 4821.056 kb on - strand at 4821.112 kb on - strand, within NIAGMN_24730 at 4821.631 kb on + strand, within NIAGMN_24735 at 4821.704 kb on + strand at 4821.732 kb on + strand at 4821.794 kb on - strand at 4821.891 kb on - strand at 4822.068 kb on + strand, within espK at 4822.763 kb on + strand, within espK at 4822.767 kb on + strand, within espK at 4822.807 kb on + strand, within espK at 4823.544 kb on + strand at 4823.544 kb on + strand at 4823.759 kb on - strand, within potB at 4823.816 kb on + strand, within potB at 4823.817 kb on - strand, within potB
Per-strain Table
Position Strand Gene LocusTag Fraction Bas14 remove 4,821,037 + -0.2 4,821,056 - +1.7 4,821,112 - NIAGMN_24730 0.27 +1.2 4,821,631 + NIAGMN_24735 0.68 -0.3 4,821,704 + +0.1 4,821,732 + -0.5 4,821,794 - +0.5 4,821,891 - +0.4 4,822,068 + espK NIAGMN_24740 0.13 -0.1 4,822,763 + espK NIAGMN_24740 0.64 -2.6 4,822,767 + espK NIAGMN_24740 0.64 -0.3 4,822,807 + espK NIAGMN_24740 0.67 -0.9 4,823,544 + +0.4 4,823,544 + +0.0 4,823,759 - potB NIAGMN_24745 0.16 +0.1 4,823,816 + potB NIAGMN_24745 0.23 +0.1 4,823,817 - potB NIAGMN_24745 0.23 +0.7
Or see this region's nucleotide sequence