Experiment: Bas14
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt murJ and flgN are separated by 39 nucleotides flgN and flgM are separated by 4 nucleotides flgM and flgA are separated by 75 nucleotides flgA and flgB are separated by 154 nucleotides
NIAGMN_24135: murJ - murein biosynthesis integral membrane protein MurJ, at 4,720,725 to 4,722,260
murJ
NIAGMN_24140: flgN - flagella biosynthesis chaperone FlgN, at 4,722,300 to 4,722,716
flgN
NIAGMN_24145: flgM - anti-sigma-28 factor FlgM, at 4,722,721 to 4,723,014
flgM
NIAGMN_24150: flgA - flagellar basal body P-ring formation protein FlgA, at 4,723,090 to 4,723,749
flgA
NIAGMN_24155: flgB - flagellar basal body rod protein FlgB, at 4,723,904 to 4,724,320
flgB
Position (kb) 4722
4723
4724 Strain fitness (log2 ratio) -1
0
1 at 4722.258 kb on + strand at 4722.258 kb on + strand at 4722.259 kb on - strand at 4722.361 kb on - strand, within flgN at 4723.592 kb on - strand, within flgA at 4723.592 kb on - strand, within flgA at 4723.634 kb on - strand, within flgA at 4723.835 kb on - strand at 4723.867 kb on - strand at 4723.903 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bas14 remove 4,722,258 + +1.7 4,722,258 + +0.7 4,722,259 - -0.1 4,722,361 - flgN NIAGMN_24140 0.15 +0.7 4,723,592 - flgA NIAGMN_24150 0.76 -0.3 4,723,592 - flgA NIAGMN_24150 0.76 +0.7 4,723,634 - flgA NIAGMN_24150 0.82 +0.7 4,723,835 - -1.5 4,723,867 - -0.3 4,723,903 + -1.1
Or see this region's nucleotide sequence