Strain Fitness in Escherichia coli ECRC102 around NIAGMN_22185

Experiment: Bas14

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntibsA and terY are separated by 220 nucleotidesterY and pphC are separated by 77 nucleotidespphC and yegI overlap by 4 nucleotides NIAGMN_22175: ibsA - Small toxic protein IbsA, at 4,371,859 to 4,371,918 ibsA NIAGMN_22180: terY - Uncharacterized protein YegL, at 4,372,139 to 4,372,798 terY NIAGMN_22185: pphC - Serine/threonine-protein phosphatase 3, at 4,372,876 to 4,373,556 pphC NIAGMN_22190: yegI - protein kinase YegI, at 4,373,553 to 4,375,493 yegI Position (kb) 4372 4373 4374Strain fitness (log2 ratio) -2 -1 0 1 2 3at 4371.888 kb on - strand, within ibsAat 4371.888 kb on - strand, within ibsAat 4371.894 kb on - strand, within ibsAat 4371.901 kb on - strand, within ibsAat 4371.916 kb on + strandat 4371.916 kb on + strandat 4371.916 kb on + strandat 4371.917 kb on - strandat 4371.917 kb on - strandat 4372.078 kb on - strandat 4372.458 kb on + strand, within terYat 4372.492 kb on + strand, within terYat 4372.636 kb on + strand, within terYat 4372.686 kb on + strand, within terYat 4372.705 kb on + strand, within terYat 4372.713 kb on + strand, within terYat 4372.813 kb on + strandat 4372.853 kb on + strandat 4372.996 kb on + strand, within pphCat 4372.996 kb on + strand, within pphCat 4373.040 kb on + strand, within pphCat 4373.346 kb on + strand, within pphCat 4373.355 kb on + strand, within pphCat 4373.523 kb on + strandat 4373.566 kb on + strandat 4373.567 kb on - strandat 4373.568 kb on + strandat 4373.810 kb on + strand, within yegIat 4373.911 kb on - strand, within yegIat 4373.911 kb on - strand, within yegIat 4373.978 kb on - strand, within yegIat 4373.993 kb on + strand, within yegIat 4373.993 kb on + strand, within yegIat 4374.279 kb on + strand, within yegIat 4374.553 kb on - strand, within yegIat 4374.553 kb on - strand, within yegI

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas14
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4,371,888 - ibsA NIAGMN_22175 0.48 +0.8
4,371,888 - ibsA NIAGMN_22175 0.48 -0.9
4,371,894 - ibsA NIAGMN_22175 0.58 -0.3
4,371,901 - ibsA NIAGMN_22175 0.70 -2.7
4,371,916 + +0.7
4,371,916 + +0.3
4,371,916 + +1.7
4,371,917 - -0.1
4,371,917 - +0.1
4,372,078 - +0.6
4,372,458 + terY NIAGMN_22180 0.48 -0.6
4,372,492 + terY NIAGMN_22180 0.53 -0.3
4,372,636 + terY NIAGMN_22180 0.75 +1.7
4,372,686 + terY NIAGMN_22180 0.83 -0.1
4,372,705 + terY NIAGMN_22180 0.86 -0.6
4,372,713 + terY NIAGMN_22180 0.87 +0.7
4,372,813 + +3.3
4,372,853 + +0.0
4,372,996 + pphC NIAGMN_22185 0.18 -0.3
4,372,996 + pphC NIAGMN_22185 0.18 +0.7
4,373,040 + pphC NIAGMN_22185 0.24 -0.2
4,373,346 + pphC NIAGMN_22185 0.69 +1.1
4,373,355 + pphC NIAGMN_22185 0.70 +0.7
4,373,523 + -0.5
4,373,566 + +0.8
4,373,567 - +1.7
4,373,568 + +0.1
4,373,810 + yegI NIAGMN_22190 0.13 +0.7
4,373,911 - yegI NIAGMN_22190 0.18 -0.6
4,373,911 - yegI NIAGMN_22190 0.18 +0.4
4,373,978 - yegI NIAGMN_22190 0.22 +0.7
4,373,993 + yegI NIAGMN_22190 0.23 +1.7
4,373,993 + yegI NIAGMN_22190 0.23 +2.7
4,374,279 + yegI NIAGMN_22190 0.37 +1.7
4,374,553 - yegI NIAGMN_22190 0.52 +1.7
4,374,553 - yegI NIAGMN_22190 0.52 +0.6

Or see this region's nucleotide sequence