Experiment: Bas14
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ycgM and maiA are separated by 8 nucleotides maiA and ubiH are separated by 14 nucleotides ubiH and dusC are separated by 159 nucleotides
NIAGMN_21365: ycgM - 5-carboxymethyl-2-hydroxymuconate isomerase, at 4,238,319 to 4,239,020
ycgM
NIAGMN_21370: maiA - maleylacetoacetate isomerase, at 4,239,029 to 4,239,673
maiA
NIAGMN_21375: ubiH - 3-hydroxybenzoate 6-monooxygenase, at 4,239,688 to 4,240,881
ubiH
NIAGMN_21380: dusC - tRNA dihydrouridine(16) synthase DusC, at 4,241,041 to 4,241,991
dusC
Position (kb) 4239
4240
4241 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 4238.789 kb on + strand, within ycgM at 4238.880 kb on + strand, within ycgM at 4238.930 kb on + strand, within ycgM at 4238.993 kb on - strand at 4239.038 kb on + strand at 4239.038 kb on + strand at 4239.052 kb on + strand at 4239.100 kb on + strand, within maiA at 4239.100 kb on + strand, within maiA at 4239.100 kb on + strand, within maiA at 4239.101 kb on - strand, within maiA at 4239.104 kb on + strand, within maiA at 4239.258 kb on - strand, within maiA at 4239.352 kb on + strand, within maiA at 4239.352 kb on + strand, within maiA at 4239.419 kb on + strand, within maiA at 4239.684 kb on + strand at 4239.684 kb on + strand at 4239.684 kb on + strand at 4239.684 kb on + strand at 4239.710 kb on + strand at 4239.912 kb on + strand, within ubiH at 4239.912 kb on + strand, within ubiH at 4239.994 kb on + strand, within ubiH at 4240.092 kb on + strand, within ubiH at 4240.140 kb on + strand, within ubiH at 4240.140 kb on + strand, within ubiH at 4240.240 kb on + strand, within ubiH at 4240.333 kb on + strand, within ubiH at 4240.357 kb on + strand, within ubiH at 4240.357 kb on + strand, within ubiH at 4240.357 kb on + strand, within ubiH at 4240.359 kb on + strand, within ubiH at 4240.360 kb on - strand, within ubiH at 4240.416 kb on + strand, within ubiH at 4240.419 kb on + strand, within ubiH at 4240.588 kb on + strand, within ubiH at 4240.588 kb on + strand, within ubiH at 4240.588 kb on + strand, within ubiH at 4240.588 kb on + strand, within ubiH at 4240.588 kb on + strand, within ubiH at 4240.588 kb on + strand, within ubiH at 4240.589 kb on - strand, within ubiH at 4240.589 kb on - strand, within ubiH at 4240.589 kb on - strand, within ubiH at 4240.590 kb on + strand, within ubiH at 4240.590 kb on + strand, within ubiH at 4240.608 kb on + strand, within ubiH at 4240.608 kb on + strand, within ubiH at 4240.628 kb on + strand, within ubiH at 4240.785 kb on + strand at 4240.786 kb on - strand at 4240.860 kb on + strand at 4240.975 kb on + strand at 4241.008 kb on - strand at 4241.040 kb on + strand at 4241.041 kb on - strand at 4241.041 kb on - strand at 4241.116 kb on + strand at 4241.300 kb on + strand, within dusC at 4241.300 kb on + strand, within dusC at 4241.398 kb on + strand, within dusC at 4241.398 kb on + strand, within dusC at 4241.580 kb on + strand, within dusC at 4241.840 kb on + strand, within dusC at 4241.842 kb on + strand, within dusC at 4241.842 kb on + strand, within dusC
Per-strain Table
Position Strand Gene LocusTag Fraction Bas14 remove 4,238,789 + ycgM NIAGMN_21365 0.67 -0.0 4,238,880 + ycgM NIAGMN_21365 0.80 +1.3 4,238,930 + ycgM NIAGMN_21365 0.87 +0.9 4,238,993 - -1.7 4,239,038 + +1.8 4,239,038 + +1.5 4,239,052 + -0.5 4,239,100 + maiA NIAGMN_21370 0.11 -0.8 4,239,100 + maiA NIAGMN_21370 0.11 -2.6 4,239,100 + maiA NIAGMN_21370 0.11 +0.4 4,239,101 - maiA NIAGMN_21370 0.11 +3.3 4,239,104 + maiA NIAGMN_21370 0.12 -1.0 4,239,258 - maiA NIAGMN_21370 0.36 -1.0 4,239,352 + maiA NIAGMN_21370 0.50 +0.5 4,239,352 + maiA NIAGMN_21370 0.50 -0.8 4,239,419 + maiA NIAGMN_21370 0.60 +0.1 4,239,684 + +1.8 4,239,684 + -0.5 4,239,684 + -0.2 4,239,684 + +0.6 4,239,710 + +1.0 4,239,912 + ubiH NIAGMN_21375 0.19 -0.4 4,239,912 + ubiH NIAGMN_21375 0.19 +0.8 4,239,994 + ubiH NIAGMN_21375 0.26 +0.1 4,240,092 + ubiH NIAGMN_21375 0.34 -0.8 4,240,140 + ubiH NIAGMN_21375 0.38 +1.0 4,240,140 + ubiH NIAGMN_21375 0.38 -0.1 4,240,240 + ubiH NIAGMN_21375 0.46 -0.9 4,240,333 + ubiH NIAGMN_21375 0.54 +0.2 4,240,357 + ubiH NIAGMN_21375 0.56 -0.8 4,240,357 + ubiH NIAGMN_21375 0.56 +1.8 4,240,357 + ubiH NIAGMN_21375 0.56 -0.7 4,240,359 + ubiH NIAGMN_21375 0.56 +0.4 4,240,360 - ubiH NIAGMN_21375 0.56 -1.4 4,240,416 + ubiH NIAGMN_21375 0.61 +0.2 4,240,419 + ubiH NIAGMN_21375 0.61 -2.6 4,240,588 + ubiH NIAGMN_21375 0.75 +1.8 4,240,588 + ubiH NIAGMN_21375 0.75 +0.8 4,240,588 + ubiH NIAGMN_21375 0.75 +2.2 4,240,588 + ubiH NIAGMN_21375 0.75 -0.4 4,240,588 + ubiH NIAGMN_21375 0.75 -1.8 4,240,588 + ubiH NIAGMN_21375 0.75 -0.0 4,240,589 - ubiH NIAGMN_21375 0.75 +1.8 4,240,589 - ubiH NIAGMN_21375 0.75 +0.2 4,240,589 - ubiH NIAGMN_21375 0.75 -0.2 4,240,590 + ubiH NIAGMN_21375 0.76 +1.9 4,240,590 + ubiH NIAGMN_21375 0.76 +0.2 4,240,608 + ubiH NIAGMN_21375 0.77 +0.5 4,240,608 + ubiH NIAGMN_21375 0.77 -2.7 4,240,628 + ubiH NIAGMN_21375 0.79 +0.9 4,240,785 + +0.8 4,240,786 - -0.8 4,240,860 + -0.5 4,240,975 + -0.9 4,241,008 - -1.1 4,241,040 + -1.1 4,241,041 - +1.3 4,241,041 - -0.9 4,241,116 + +0.4 4,241,300 + dusC NIAGMN_21380 0.27 +2.5 4,241,300 + dusC NIAGMN_21380 0.27 +0.9 4,241,398 + dusC NIAGMN_21380 0.38 +1.4 4,241,398 + dusC NIAGMN_21380 0.38 +0.9 4,241,580 + dusC NIAGMN_21380 0.57 +0.8 4,241,840 + dusC NIAGMN_21380 0.84 -0.8 4,241,842 + dusC NIAGMN_21380 0.84 +0.2 4,241,842 + dusC NIAGMN_21380 0.84 +1.8
Or see this region's nucleotide sequence