Strain Fitness in Escherichia coli ECRC102 around NIAGMN_20905

Experiment: Bas14

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntgyrA and yfaA are separated by 282 nucleotidesyfaA and yfaT overlap by 4 nucleotidesyfaT and yfaS are separated by 143 nucleotides NIAGMN_20900: gyrA - DNA topoisomerase (ATP-hydrolyzing) subunit A, at 4,135,168 to 4,137,795 gyrA NIAGMN_20905: yfaA - DUF2138 domain-containing protein, at 4,138,078 to 4,139,631 yfaA NIAGMN_20910: yfaT - Uncharacterized protein YfaT, at 4,139,628 to 4,140,251 yfaT NIAGMN_20915: yfaS - Uncharacterized conserved protein YfaS, alpha-2-macroglobulin family, at 4,140,395 to 4,144,789 yfaS Position (kb) 4138 4139 4140Strain fitness (log2 ratio) -2 -1 0 1 2 3at 4137.793 kb on + strandat 4137.799 kb on + strandat 4138.213 kb on + strandat 4138.372 kb on + strand, within yfaAat 4138.372 kb on + strand, within yfaAat 4138.373 kb on - strand, within yfaAat 4138.494 kb on + strand, within yfaAat 4138.549 kb on + strand, within yfaAat 4138.829 kb on + strand, within yfaAat 4138.830 kb on - strand, within yfaAat 4138.850 kb on + strand, within yfaAat 4138.850 kb on + strand, within yfaAat 4138.851 kb on - strand, within yfaAat 4138.851 kb on - strand, within yfaAat 4138.887 kb on + strand, within yfaAat 4139.388 kb on - strand, within yfaAat 4139.388 kb on - strand, within yfaAat 4139.388 kb on - strand, within yfaAat 4139.417 kb on - strand, within yfaAat 4139.419 kb on - strand, within yfaAat 4139.516 kb on + strandat 4139.558 kb on - strandat 4139.612 kb on + strandat 4139.877 kb on + strand, within yfaTat 4139.962 kb on - strand, within yfaTat 4139.989 kb on - strand, within yfaTat 4139.989 kb on - strand, within yfaTat 4140.017 kb on - strand, within yfaTat 4140.074 kb on - strand, within yfaTat 4140.074 kb on - strand, within yfaTat 4140.094 kb on - strand, within yfaTat 4140.103 kb on - strand, within yfaTat 4140.164 kb on + strand, within yfaTat 4140.165 kb on - strand, within yfaTat 4140.165 kb on - strand, within yfaTat 4140.323 kb on + strandat 4140.323 kb on + strandat 4140.324 kb on - strandat 4140.471 kb on - strandat 4140.476 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas14
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4,137,793 + +0.2
4,137,799 + -0.2
4,138,213 + -0.0
4,138,372 + yfaA NIAGMN_20905 0.19 +0.4
4,138,372 + yfaA NIAGMN_20905 0.19 +0.5
4,138,373 - yfaA NIAGMN_20905 0.19 +1.5
4,138,494 + yfaA NIAGMN_20905 0.27 -0.5
4,138,549 + yfaA NIAGMN_20905 0.30 -2.6
4,138,829 + yfaA NIAGMN_20905 0.48 -0.1
4,138,830 - yfaA NIAGMN_20905 0.48 +1.8
4,138,850 + yfaA NIAGMN_20905 0.50 +0.1
4,138,850 + yfaA NIAGMN_20905 0.50 -0.3
4,138,851 - yfaA NIAGMN_20905 0.50 +1.6
4,138,851 - yfaA NIAGMN_20905 0.50 +0.8
4,138,887 + yfaA NIAGMN_20905 0.52 +0.2
4,139,388 - yfaA NIAGMN_20905 0.84 -0.2
4,139,388 - yfaA NIAGMN_20905 0.84 -0.6
4,139,388 - yfaA NIAGMN_20905 0.84 -0.2
4,139,417 - yfaA NIAGMN_20905 0.86 +2.8
4,139,419 - yfaA NIAGMN_20905 0.86 -1.2
4,139,516 + -1.6
4,139,558 - +0.1
4,139,612 + +0.8
4,139,877 + yfaT NIAGMN_20910 0.40 +0.2
4,139,962 - yfaT NIAGMN_20910 0.54 -0.8
4,139,989 - yfaT NIAGMN_20910 0.58 +0.6
4,139,989 - yfaT NIAGMN_20910 0.58 +0.8
4,140,017 - yfaT NIAGMN_20910 0.62 -1.7
4,140,074 - yfaT NIAGMN_20910 0.71 +0.1
4,140,074 - yfaT NIAGMN_20910 0.71 +0.2
4,140,094 - yfaT NIAGMN_20910 0.75 +3.2
4,140,103 - yfaT NIAGMN_20910 0.76 -0.2
4,140,164 + yfaT NIAGMN_20910 0.86 +0.5
4,140,165 - yfaT NIAGMN_20910 0.86 -1.0
4,140,165 - yfaT NIAGMN_20910 0.86 +1.8
4,140,323 + +0.5
4,140,323 + -1.2
4,140,324 - +2.1
4,140,471 - +1.6
4,140,476 - +0.8

Or see this region's nucleotide sequence