Experiment: Bas14
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hisM and hisP are separated by 7 nucleotides hisP and rpnB are separated by 196 nucleotides rpnB and yfcH are separated by 47 nucleotides yfcH and folX are separated by 20 nucleotides
NIAGMN_20510: hisM - Histidine transport system permease protein HisM, at 4,050,268 to 4,050,984
hisM
NIAGMN_20515: hisP - histidine ABC transporter ATP-binding protein HisP, at 4,050,992 to 4,051,765
hisP
NIAGMN_20520: rpnB - recombination-promoting nuclease RpnB, at 4,051,962 to 4,052,852
rpnB
NIAGMN_20525: yfcH - Epimerase family protein YfcH, at 4,052,900 to 4,053,793
yfcH
NIAGMN_20530: folX - dihydroneopterin triphosphate 2'-epimerase, at 4,053,814 to 4,054,176
folX
Position (kb) 4051
4052
4053 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 4051.003 kb on + strand at 4051.089 kb on + strand, within hisP at 4051.089 kb on + strand, within hisP at 4051.144 kb on + strand, within hisP at 4051.257 kb on + strand, within hisP at 4051.309 kb on + strand, within hisP at 4051.309 kb on + strand, within hisP at 4051.394 kb on + strand, within hisP at 4051.413 kb on + strand, within hisP at 4051.573 kb on + strand, within hisP at 4051.644 kb on + strand, within hisP at 4051.644 kb on + strand, within hisP at 4051.645 kb on - strand, within hisP at 4051.912 kb on - strand at 4052.142 kb on + strand, within rpnB at 4052.435 kb on + strand, within rpnB at 4052.436 kb on - strand, within rpnB at 4052.462 kb on + strand, within rpnB at 4052.600 kb on + strand, within rpnB at 4052.600 kb on + strand, within rpnB at 4052.611 kb on + strand, within rpnB at 4052.645 kb on + strand, within rpnB at 4052.656 kb on + strand, within rpnB at 4052.975 kb on - strand at 4052.996 kb on + strand, within yfcH at 4052.996 kb on + strand, within yfcH at 4052.997 kb on - strand, within yfcH at 4052.997 kb on - strand, within yfcH at 4052.997 kb on - strand, within yfcH at 4052.997 kb on - strand, within yfcH at 4052.997 kb on - strand, within yfcH at 4053.134 kb on - strand, within yfcH at 4053.210 kb on + strand, within yfcH at 4053.432 kb on - strand, within yfcH at 4053.432 kb on - strand, within yfcH at 4053.432 kb on - strand, within yfcH at 4053.445 kb on - strand, within yfcH at 4053.445 kb on - strand, within yfcH at 4053.445 kb on - strand, within yfcH at 4053.761 kb on + strand at 4053.786 kb on - strand at 4053.786 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bas14 remove 4,051,003 + +1.8 4,051,089 + hisP NIAGMN_20515 0.13 -0.8 4,051,089 + hisP NIAGMN_20515 0.13 +0.8 4,051,144 + hisP NIAGMN_20515 0.20 +0.8 4,051,257 + hisP NIAGMN_20515 0.34 +0.4 4,051,309 + hisP NIAGMN_20515 0.41 +0.8 4,051,309 + hisP NIAGMN_20515 0.41 +0.8 4,051,394 + hisP NIAGMN_20515 0.52 +0.1 4,051,413 + hisP NIAGMN_20515 0.54 +0.4 4,051,573 + hisP NIAGMN_20515 0.75 +2.2 4,051,644 + hisP NIAGMN_20515 0.84 +0.8 4,051,644 + hisP NIAGMN_20515 0.84 -0.2 4,051,645 - hisP NIAGMN_20515 0.84 -2.1 4,051,912 - +2.8 4,052,142 + rpnB NIAGMN_20520 0.20 -1.0 4,052,435 + rpnB NIAGMN_20520 0.53 +0.2 4,052,436 - rpnB NIAGMN_20520 0.53 -0.4 4,052,462 + rpnB NIAGMN_20520 0.56 -0.8 4,052,600 + rpnB NIAGMN_20520 0.72 +0.8 4,052,600 + rpnB NIAGMN_20520 0.72 +0.3 4,052,611 + rpnB NIAGMN_20520 0.73 -0.9 4,052,645 + rpnB NIAGMN_20520 0.77 +1.8 4,052,656 + rpnB NIAGMN_20520 0.78 +1.8 4,052,975 - -0.6 4,052,996 + yfcH NIAGMN_20525 0.11 +0.8 4,052,996 + yfcH NIAGMN_20525 0.11 -0.6 4,052,997 - yfcH NIAGMN_20525 0.11 +1.8 4,052,997 - yfcH NIAGMN_20525 0.11 +0.2 4,052,997 - yfcH NIAGMN_20525 0.11 +0.6 4,052,997 - yfcH NIAGMN_20525 0.11 +0.2 4,052,997 - yfcH NIAGMN_20525 0.11 +0.1 4,053,134 - yfcH NIAGMN_20525 0.26 +1.3 4,053,210 + yfcH NIAGMN_20525 0.35 +1.8 4,053,432 - yfcH NIAGMN_20525 0.60 -1.2 4,053,432 - yfcH NIAGMN_20525 0.60 -1.5 4,053,432 - yfcH NIAGMN_20525 0.60 -1.5 4,053,445 - yfcH NIAGMN_20525 0.61 +1.2 4,053,445 - yfcH NIAGMN_20525 0.61 -0.2 4,053,445 - yfcH NIAGMN_20525 0.61 -1.5 4,053,761 + -1.0 4,053,786 - -0.8 4,053,786 - +0.0
Or see this region's nucleotide sequence