Strain Fitness in Escherichia coli ECRC102 around NIAGMN_19635

Experiment: Bas14

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nthyfJ and hyfI overlap by 8 nucleotideshyfI and hyfH overlap by 4 nucleotideshyfH and hyfG are separated by 9 nucleotides NIAGMN_19625: hyfJ - hydrogenase 4 assembly chaperone HyfJ, at 3,873,658 to 3,874,071 hyfJ NIAGMN_19630: hyfI - hydrogenase 4 catalytic subunit HyfI, at 3,874,064 to 3,874,822 hyfI NIAGMN_19635: hyfH - hydrogenase 4 subunit H, at 3,874,819 to 3,875,364 hyfH NIAGMN_19640: hyfG - hydrogenase 4 catalytic subunit HyfG, at 3,875,374 to 3,877,089 hyfG Position (kb) 3874 3875 3876Strain fitness (log2 ratio) -2 -1 0 1 2at 3873.852 kb on + strand, within hyfJat 3873.853 kb on - strand, within hyfJat 3873.974 kb on + strand, within hyfJat 3873.974 kb on + strand, within hyfJat 3873.975 kb on - strand, within hyfJat 3873.975 kb on - strand, within hyfJat 3873.975 kb on - strand, within hyfJat 3873.975 kb on - strand, within hyfJat 3874.074 kb on - strandat 3874.108 kb on + strandat 3874.108 kb on + strandat 3874.111 kb on + strandat 3874.178 kb on + strand, within hyfIat 3874.178 kb on + strand, within hyfIat 3874.178 kb on + strand, within hyfIat 3874.178 kb on + strand, within hyfIat 3874.297 kb on + strand, within hyfIat 3874.298 kb on - strand, within hyfIat 3874.298 kb on - strand, within hyfIat 3874.536 kb on + strand, within hyfIat 3874.537 kb on - strand, within hyfIat 3874.537 kb on - strand, within hyfIat 3874.555 kb on + strand, within hyfIat 3874.556 kb on - strand, within hyfIat 3874.583 kb on - strand, within hyfIat 3874.583 kb on - strand, within hyfIat 3874.583 kb on - strand, within hyfIat 3874.807 kb on + strandat 3874.808 kb on - strandat 3874.808 kb on - strandat 3874.822 kb on + strandat 3875.024 kb on - strand, within hyfHat 3875.082 kb on + strand, within hyfHat 3875.153 kb on + strand, within hyfHat 3875.197 kb on - strand, within hyfHat 3875.244 kb on + strand, within hyfHat 3875.244 kb on + strand, within hyfHat 3875.344 kb on - strandat 3875.375 kb on + strandat 3875.375 kb on + strandat 3875.426 kb on + strandat 3875.426 kb on + strandat 3875.427 kb on - strandat 3875.489 kb on + strandat 3875.552 kb on + strand, within hyfGat 3875.553 kb on - strand, within hyfGat 3875.577 kb on - strand, within hyfGat 3875.692 kb on - strand, within hyfGat 3875.693 kb on + strand, within hyfGat 3875.698 kb on - strand, within hyfGat 3875.698 kb on - strand, within hyfGat 3875.698 kb on - strand, within hyfGat 3875.824 kb on - strand, within hyfGat 3875.824 kb on - strand, within hyfGat 3875.824 kb on - strand, within hyfGat 3875.824 kb on - strand, within hyfGat 3875.924 kb on + strand, within hyfGat 3876.111 kb on - strand, within hyfGat 3876.180 kb on + strand, within hyfGat 3876.181 kb on - strand, within hyfGat 3876.241 kb on + strand, within hyfGat 3876.328 kb on - strand, within hyfGat 3876.330 kb on - strand, within hyfG

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas14
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3,873,852 + hyfJ NIAGMN_19625 0.47 +0.6
3,873,853 - hyfJ NIAGMN_19625 0.47 -0.9
3,873,974 + hyfJ NIAGMN_19625 0.76 +0.5
3,873,974 + hyfJ NIAGMN_19625 0.76 +1.7
3,873,975 - hyfJ NIAGMN_19625 0.77 -0.4
3,873,975 - hyfJ NIAGMN_19625 0.77 -1.3
3,873,975 - hyfJ NIAGMN_19625 0.77 -0.3
3,873,975 - hyfJ NIAGMN_19625 0.77 -0.4
3,874,074 - -1.7
3,874,108 + -0.4
3,874,108 + -0.8
3,874,111 + -0.2
3,874,178 + hyfI NIAGMN_19630 0.15 -1.1
3,874,178 + hyfI NIAGMN_19630 0.15 -1.0
3,874,178 + hyfI NIAGMN_19630 0.15 +0.9
3,874,178 + hyfI NIAGMN_19630 0.15 -0.4
3,874,297 + hyfI NIAGMN_19630 0.31 -0.3
3,874,298 - hyfI NIAGMN_19630 0.31 +0.2
3,874,298 - hyfI NIAGMN_19630 0.31 +0.3
3,874,536 + hyfI NIAGMN_19630 0.62 -0.2
3,874,537 - hyfI NIAGMN_19630 0.62 +2.4
3,874,537 - hyfI NIAGMN_19630 0.62 -0.5
3,874,555 + hyfI NIAGMN_19630 0.65 -2.0
3,874,556 - hyfI NIAGMN_19630 0.65 +1.4
3,874,583 - hyfI NIAGMN_19630 0.68 -0.1
3,874,583 - hyfI NIAGMN_19630 0.68 +0.9
3,874,583 - hyfI NIAGMN_19630 0.68 +0.3
3,874,807 + +1.9
3,874,808 - +1.9
3,874,808 - -0.4
3,874,822 + +0.6
3,875,024 - hyfH NIAGMN_19635 0.38 +0.1
3,875,082 + hyfH NIAGMN_19635 0.48 +2.3
3,875,153 + hyfH NIAGMN_19635 0.61 +0.3
3,875,197 - hyfH NIAGMN_19635 0.69 -0.1
3,875,244 + hyfH NIAGMN_19635 0.78 +1.3
3,875,244 + hyfH NIAGMN_19635 0.78 +0.9
3,875,344 - -1.2
3,875,375 + +0.9
3,875,375 + +0.9
3,875,426 + +1.2
3,875,426 + -0.9
3,875,427 - +0.5
3,875,489 + +2.6
3,875,552 + hyfG NIAGMN_19640 0.10 +0.7
3,875,553 - hyfG NIAGMN_19640 0.10 +1.2
3,875,577 - hyfG NIAGMN_19640 0.12 -0.7
3,875,692 - hyfG NIAGMN_19640 0.19 -0.4
3,875,693 + hyfG NIAGMN_19640 0.19 +0.3
3,875,698 - hyfG NIAGMN_19640 0.19 -0.9
3,875,698 - hyfG NIAGMN_19640 0.19 -0.9
3,875,698 - hyfG NIAGMN_19640 0.19 +0.9
3,875,824 - hyfG NIAGMN_19640 0.26 +2.2
3,875,824 - hyfG NIAGMN_19640 0.26 -1.9
3,875,824 - hyfG NIAGMN_19640 0.26 +0.2
3,875,824 - hyfG NIAGMN_19640 0.26 -0.9
3,875,924 + hyfG NIAGMN_19640 0.32 +1.2
3,876,111 - hyfG NIAGMN_19640 0.43 +0.3
3,876,180 + hyfG NIAGMN_19640 0.47 -2.0
3,876,181 - hyfG NIAGMN_19640 0.47 +0.3
3,876,241 + hyfG NIAGMN_19640 0.51 +1.4
3,876,328 - hyfG NIAGMN_19640 0.56 +1.9
3,876,330 - hyfG NIAGMN_19640 0.56 -0.4

Or see this region's nucleotide sequence