Experiment: Bas14
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt truB and rpsO are separated by 148 nucleotides rpsO and pnp are separated by 246 nucleotides pnp and nlpI are separated by 108 nucleotides
NIAGMN_15885: truB - tRNA pseudouridine(55) synthase TruB, at 3,146,455 to 3,147,399
truB
NIAGMN_15890: rpsO - 30S ribosomal protein S15, at 3,147,548 to 3,147,817
rpsO
NIAGMN_15900: pnp - polyribonucleotide nucleotidyltransferase, at 3,148,064 to 3,150,199
pnp
NIAGMN_15905: nlpI - lipoprotein NlpI, at 3,150,308 to 3,151,192
nlpI
Position (kb)
3148
3149
3150
3151 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 3147.072 kb on + strand, within truB at 3147.073 kb on - strand, within truB at 3147.073 kb on - strand, within truB at 3147.073 kb on - strand, within truB at 3147.174 kb on - strand, within truB at 3147.267 kb on + strand, within truB at 3147.268 kb on - strand, within truB at 3147.397 kb on + strand at 3147.397 kb on + strand at 3147.468 kb on + strand at 3147.485 kb on + strand at 3147.891 kb on + strand at 3147.891 kb on + strand at 3147.891 kb on + strand at 3147.896 kb on + strand at 3147.896 kb on + strand at 3147.896 kb on + strand at 3147.896 kb on + strand at 3147.905 kb on + strand at 3147.905 kb on + strand at 3147.905 kb on + strand at 3147.905 kb on + strand at 3147.961 kb on + strand at 3147.961 kb on + strand at 3147.961 kb on + strand at 3147.961 kb on + strand at 3147.961 kb on + strand at 3147.972 kb on + strand at 3148.029 kb on + strand at 3149.956 kb on + strand, within pnp at 3150.131 kb on - strand at 3150.197 kb on + strand at 3150.197 kb on + strand at 3150.197 kb on + strand at 3150.198 kb on - strand at 3150.237 kb on - strand at 3150.257 kb on + strand at 3150.349 kb on + strand at 3150.349 kb on + strand at 3150.349 kb on + strand at 3150.349 kb on + strand at 3150.350 kb on - strand at 3150.350 kb on - strand at 3150.368 kb on - strand at 3150.368 kb on - strand at 3150.370 kb on + strand at 3150.370 kb on + strand at 3150.370 kb on + strand at 3150.370 kb on + strand at 3150.371 kb on - strand at 3150.371 kb on - strand at 3150.382 kb on + strand at 3150.383 kb on - strand at 3150.420 kb on + strand, within nlpI at 3150.421 kb on - strand, within nlpI at 3150.445 kb on + strand, within nlpI at 3150.446 kb on - strand, within nlpI at 3150.501 kb on + strand, within nlpI at 3150.501 kb on + strand, within nlpI at 3150.501 kb on + strand, within nlpI at 3150.501 kb on + strand, within nlpI at 3150.521 kb on + strand, within nlpI at 3150.521 kb on + strand, within nlpI at 3150.521 kb on + strand, within nlpI at 3150.600 kb on + strand, within nlpI at 3150.608 kb on + strand, within nlpI at 3150.608 kb on + strand, within nlpI at 3150.618 kb on + strand, within nlpI at 3150.618 kb on + strand, within nlpI at 3150.618 kb on + strand, within nlpI at 3150.620 kb on + strand, within nlpI at 3150.620 kb on + strand, within nlpI at 3150.620 kb on + strand, within nlpI at 3150.620 kb on + strand, within nlpI at 3150.653 kb on + strand, within nlpI at 3150.672 kb on + strand, within nlpI at 3150.692 kb on + strand, within nlpI at 3150.692 kb on + strand, within nlpI at 3150.698 kb on + strand, within nlpI at 3150.698 kb on + strand, within nlpI at 3150.698 kb on + strand, within nlpI at 3150.698 kb on + strand, within nlpI at 3150.698 kb on + strand, within nlpI at 3150.726 kb on + strand, within nlpI at 3150.728 kb on + strand, within nlpI at 3150.731 kb on + strand, within nlpI at 3150.750 kb on + strand, within nlpI at 3150.818 kb on + strand, within nlpI at 3150.853 kb on + strand, within nlpI at 3150.982 kb on + strand, within nlpI at 3151.022 kb on + strand, within nlpI at 3151.022 kb on + strand, within nlpI at 3151.022 kb on + strand, within nlpI at 3151.022 kb on + strand, within nlpI at 3151.022 kb on + strand, within nlpI at 3151.022 kb on + strand, within nlpI at 3151.026 kb on + strand, within nlpI at 3151.026 kb on + strand, within nlpI at 3151.026 kb on + strand, within nlpI at 3151.026 kb on + strand, within nlpI at 3151.027 kb on - strand, within nlpI at 3151.030 kb on + strand, within nlpI at 3151.030 kb on + strand, within nlpI at 3151.030 kb on + strand, within nlpI at 3151.034 kb on + strand, within nlpI at 3151.034 kb on + strand, within nlpI at 3151.034 kb on + strand, within nlpI at 3151.034 kb on + strand, within nlpI at 3151.034 kb on + strand, within nlpI at 3151.037 kb on + strand, within nlpI at 3151.041 kb on + strand, within nlpI at 3151.041 kb on + strand, within nlpI at 3151.041 kb on + strand, within nlpI at 3151.041 kb on + strand, within nlpI at 3151.105 kb on + strand at 3151.105 kb on + strand at 3151.190 kb on + strand at 3151.190 kb on + strand at 3151.190 kb on + strand at 3151.190 kb on + strand at 3151.199 kb on + strand at 3151.199 kb on + strand at 3151.199 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bas14 remove 3,147,072 + truB NIAGMN_15885 0.65 -0.4 3,147,073 - truB NIAGMN_15885 0.65 +1.1 3,147,073 - truB NIAGMN_15885 0.65 -1.3 3,147,073 - truB NIAGMN_15885 0.65 +1.1 3,147,174 - truB NIAGMN_15885 0.76 +1.1 3,147,267 + truB NIAGMN_15885 0.86 +0.1 3,147,268 - truB NIAGMN_15885 0.86 -0.1 3,147,397 + -0.6 3,147,397 + -0.9 3,147,468 + +0.1 3,147,485 + +2.2 3,147,891 + -0.7 3,147,891 + +1.3 3,147,891 + +0.4 3,147,896 + +0.1 3,147,896 + +0.3 3,147,896 + -0.7 3,147,896 + -1.0 3,147,905 + -0.9 3,147,905 + +0.4 3,147,905 + -0.5 3,147,905 + +4.7 3,147,961 + +1.1 3,147,961 + +0.7 3,147,961 + -2.2 3,147,961 + +1.1 3,147,961 + +0.5 3,147,972 + -1.7 3,148,029 + -0.2 3,149,956 + pnp NIAGMN_15900 0.89 +1.3 3,150,131 - +0.5 3,150,197 + -0.3 3,150,197 + -0.7 3,150,197 + -0.3 3,150,198 - +1.0 3,150,237 - +0.5 3,150,257 + -0.7 3,150,349 + +1.7 3,150,349 + -1.6 3,150,349 + -0.3 3,150,349 + -0.7 3,150,350 - +0.1 3,150,350 - -0.3 3,150,368 - +3.1 3,150,368 - +2.1 3,150,370 + -0.3 3,150,370 + +3.7 3,150,370 + -0.1 3,150,370 + +0.3 3,150,371 - +3.1 3,150,371 - -0.4 3,150,382 + +0.6 3,150,383 - -0.3 3,150,420 + nlpI NIAGMN_15905 0.13 +0.7 3,150,421 - nlpI NIAGMN_15905 0.13 -0.2 3,150,445 + nlpI NIAGMN_15905 0.15 +0.5 3,150,446 - nlpI NIAGMN_15905 0.16 +2.1 3,150,501 + nlpI NIAGMN_15905 0.22 -1.3 3,150,501 + nlpI NIAGMN_15905 0.22 -0.0 3,150,501 + nlpI NIAGMN_15905 0.22 +2.7 3,150,501 + nlpI NIAGMN_15905 0.22 -0.1 3,150,521 + nlpI NIAGMN_15905 0.24 +2.1 3,150,521 + nlpI NIAGMN_15905 0.24 +0.7 3,150,521 + nlpI NIAGMN_15905 0.24 +3.1 3,150,600 + nlpI NIAGMN_15905 0.33 +0.3 3,150,608 + nlpI NIAGMN_15905 0.34 +0.2 3,150,608 + nlpI NIAGMN_15905 0.34 -1.1 3,150,618 + nlpI NIAGMN_15905 0.35 +0.3 3,150,618 + nlpI NIAGMN_15905 0.35 +0.1 3,150,618 + nlpI NIAGMN_15905 0.35 -0.1 3,150,620 + nlpI NIAGMN_15905 0.35 -0.4 3,150,620 + nlpI NIAGMN_15905 0.35 -0.3 3,150,620 + nlpI NIAGMN_15905 0.35 +0.1 3,150,620 + nlpI NIAGMN_15905 0.35 -1.1 3,150,653 + nlpI NIAGMN_15905 0.39 +1.1 3,150,672 + nlpI NIAGMN_15905 0.41 -0.0 3,150,692 + nlpI NIAGMN_15905 0.43 +0.2 3,150,692 + nlpI NIAGMN_15905 0.43 +0.2 3,150,698 + nlpI NIAGMN_15905 0.44 -0.8 3,150,698 + nlpI NIAGMN_15905 0.44 -0.9 3,150,698 + nlpI NIAGMN_15905 0.44 +0.5 3,150,698 + nlpI NIAGMN_15905 0.44 +0.1 3,150,698 + nlpI NIAGMN_15905 0.44 +2.0 3,150,726 + nlpI NIAGMN_15905 0.47 +0.3 3,150,728 + nlpI NIAGMN_15905 0.47 -0.1 3,150,731 + nlpI NIAGMN_15905 0.48 -0.1 3,150,750 + nlpI NIAGMN_15905 0.50 +0.2 3,150,818 + nlpI NIAGMN_15905 0.58 -0.1 3,150,853 + nlpI NIAGMN_15905 0.62 +0.1 3,150,982 + nlpI NIAGMN_15905 0.76 +1.9 3,151,022 + nlpI NIAGMN_15905 0.81 -0.1 3,151,022 + nlpI NIAGMN_15905 0.81 +0.5 3,151,022 + nlpI NIAGMN_15905 0.81 +0.1 3,151,022 + nlpI NIAGMN_15905 0.81 +0.1 3,151,022 + nlpI NIAGMN_15905 0.81 -1.3 3,151,022 + nlpI NIAGMN_15905 0.81 +1.7 3,151,026 + nlpI NIAGMN_15905 0.81 -0.3 3,151,026 + nlpI NIAGMN_15905 0.81 -0.3 3,151,026 + nlpI NIAGMN_15905 0.81 -0.9 3,151,026 + nlpI NIAGMN_15905 0.81 +0.8 3,151,027 - nlpI NIAGMN_15905 0.81 +0.1 3,151,030 + nlpI NIAGMN_15905 0.82 -0.0 3,151,030 + nlpI NIAGMN_15905 0.82 -0.9 3,151,030 + nlpI NIAGMN_15905 0.82 +0.5 3,151,034 + nlpI NIAGMN_15905 0.82 +0.4 3,151,034 + nlpI NIAGMN_15905 0.82 +1.3 3,151,034 + nlpI NIAGMN_15905 0.82 +2.1 3,151,034 + nlpI NIAGMN_15905 0.82 +0.9 3,151,034 + nlpI NIAGMN_15905 0.82 +0.3 3,151,037 + nlpI NIAGMN_15905 0.82 +0.2 3,151,041 + nlpI NIAGMN_15905 0.83 -0.3 3,151,041 + nlpI NIAGMN_15905 0.83 +0.8 3,151,041 + nlpI NIAGMN_15905 0.83 -1.3 3,151,041 + nlpI NIAGMN_15905 0.83 +0.5 3,151,105 + +0.5 3,151,105 + -0.6 3,151,190 + -1.1 3,151,190 + -0.7 3,151,190 + +0.3 3,151,190 + +1.0 3,151,199 + -0.5 3,151,199 + +0.0 3,151,199 + +0.1
Or see this region's nucleotide sequence