Experiment: Bas14
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt damX and dam are separated by 106 nucleotides dam and dam are separated by 17 nucleotides dam and gph overlap by 8 nucleotides gph and trpS overlap by 8 nucleotides
NIAGMN_14890: damX - cell division protein DamX, at 2,972,227 to 2,973,513
damX
NIAGMN_14895: dam - adenine-specific DNA-methyltransferase, at 2,973,620 to 2,974,456
dam
NIAGMN_14900: dam - adenine-specific DNA-methyltransferase, at 2,974,474 to 2,975,151
dam
NIAGMN_14905: gph - phosphoglycolate phosphatase, at 2,975,144 to 2,975,902
gph
NIAGMN_14910: trpS - tryptophan--tRNA ligase, at 2,975,895 to 2,976,899
trpS
Position (kb)
2974
2975
2976 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2973.511 kb on + strand at 2973.511 kb on + strand at 2973.511 kb on + strand at 2973.521 kb on + strand at 2973.538 kb on + strand at 2973.538 kb on + strand at 2973.604 kb on + strand at 2973.604 kb on + strand at 2973.604 kb on + strand at 2973.605 kb on - strand at 2973.605 kb on - strand at 2973.608 kb on + strand at 2973.608 kb on + strand at 2973.608 kb on + strand at 2973.612 kb on + strand at 2975.138 kb on + strand at 2975.149 kb on + strand at 2975.150 kb on - strand at 2975.162 kb on - strand at 2975.233 kb on + strand, within gph at 2975.233 kb on + strand, within gph at 2975.243 kb on + strand, within gph at 2975.281 kb on + strand, within gph at 2975.284 kb on + strand, within gph at 2975.297 kb on - strand, within gph at 2975.374 kb on + strand, within gph at 2975.415 kb on + strand, within gph at 2975.416 kb on - strand, within gph at 2975.416 kb on - strand, within gph at 2975.416 kb on - strand, within gph at 2975.419 kb on + strand, within gph at 2975.419 kb on + strand, within gph at 2975.419 kb on + strand, within gph at 2975.420 kb on - strand, within gph at 2975.420 kb on - strand, within gph at 2975.420 kb on - strand, within gph at 2975.420 kb on - strand, within gph at 2975.420 kb on - strand, within gph at 2975.420 kb on - strand, within gph at 2975.428 kb on + strand, within gph at 2975.429 kb on - strand, within gph at 2975.444 kb on + strand, within gph at 2975.448 kb on - strand, within gph at 2975.448 kb on - strand, within gph at 2975.497 kb on + strand, within gph at 2975.497 kb on + strand, within gph at 2975.498 kb on - strand, within gph at 2975.586 kb on + strand, within gph at 2975.586 kb on + strand, within gph at 2975.586 kb on + strand, within gph at 2975.587 kb on - strand, within gph at 2975.587 kb on - strand, within gph at 2975.587 kb on - strand, within gph at 2975.587 kb on - strand, within gph at 2975.587 kb on - strand, within gph at 2975.600 kb on + strand, within gph at 2975.732 kb on - strand, within gph at 2975.772 kb on + strand, within gph at 2975.772 kb on + strand, within gph at 2975.778 kb on - strand, within gph at 2975.785 kb on + strand, within gph at 2975.785 kb on + strand, within gph at 2975.785 kb on + strand, within gph at 2975.785 kb on + strand, within gph at 2975.785 kb on + strand, within gph at 2975.785 kb on + strand, within gph at 2975.785 kb on + strand, within gph at 2975.785 kb on + strand, within gph at 2975.785 kb on + strand, within gph at 2975.786 kb on - strand, within gph at 2975.786 kb on - strand, within gph at 2975.786 kb on - strand, within gph at 2975.786 kb on - strand, within gph at 2975.786 kb on - strand, within gph at 2975.786 kb on - strand, within gph at 2975.786 kb on - strand, within gph at 2975.786 kb on - strand, within gph at 2975.786 kb on - strand, within gph at 2975.786 kb on - strand, within gph at 2975.786 kb on - strand, within gph at 2975.786 kb on - strand, within gph at 2975.786 kb on - strand, within gph at 2975.789 kb on + strand, within gph at 2975.789 kb on + strand, within gph at 2975.789 kb on + strand, within gph at 2975.789 kb on + strand, within gph at 2975.800 kb on + strand, within gph at 2975.823 kb on + strand, within gph at 2975.823 kb on + strand, within gph at 2975.828 kb on + strand at 2975.828 kb on + strand at 2975.836 kb on + strand at 2975.838 kb on + strand at 2975.838 kb on + strand at 2975.838 kb on + strand at 2975.838 kb on + strand at 2975.838 kb on + strand at 2975.838 kb on + strand at 2975.838 kb on + strand at 2975.859 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bas14 remove 2,973,511 + -0.4 2,973,511 + -0.6 2,973,511 + -0.7 2,973,521 + +0.9 2,973,538 + +0.3 2,973,538 + -0.5 2,973,604 + +0.0 2,973,604 + -0.1 2,973,604 + +0.6 2,973,605 - -0.3 2,973,605 - -0.3 2,973,608 + +0.1 2,973,608 + -0.5 2,973,608 + -2.0 2,973,612 + -0.3 2,975,138 + -1.3 2,975,149 + -1.0 2,975,150 - +0.5 2,975,162 - -1.4 2,975,233 + gph NIAGMN_14905 0.12 +0.3 2,975,233 + gph NIAGMN_14905 0.12 +0.9 2,975,243 + gph NIAGMN_14905 0.13 +0.5 2,975,281 + gph NIAGMN_14905 0.18 +1.0 2,975,284 + gph NIAGMN_14905 0.18 -0.3 2,975,297 - gph NIAGMN_14905 0.20 -0.3 2,975,374 + gph NIAGMN_14905 0.30 -2.0 2,975,415 + gph NIAGMN_14905 0.36 -0.9 2,975,416 - gph NIAGMN_14905 0.36 +2.7 2,975,416 - gph NIAGMN_14905 0.36 -1.0 2,975,416 - gph NIAGMN_14905 0.36 +0.2 2,975,419 + gph NIAGMN_14905 0.36 +0.5 2,975,419 + gph NIAGMN_14905 0.36 +1.2 2,975,419 + gph NIAGMN_14905 0.36 +0.3 2,975,420 - gph NIAGMN_14905 0.36 +0.1 2,975,420 - gph NIAGMN_14905 0.36 +1.6 2,975,420 - gph NIAGMN_14905 0.36 -1.5 2,975,420 - gph NIAGMN_14905 0.36 -0.5 2,975,420 - gph NIAGMN_14905 0.36 +1.0 2,975,420 - gph NIAGMN_14905 0.36 +0.1 2,975,428 + gph NIAGMN_14905 0.37 +0.5 2,975,429 - gph NIAGMN_14905 0.38 +1.5 2,975,444 + gph NIAGMN_14905 0.40 +0.3 2,975,448 - gph NIAGMN_14905 0.40 +0.8 2,975,448 - gph NIAGMN_14905 0.40 -0.7 2,975,497 + gph NIAGMN_14905 0.47 -0.0 2,975,497 + gph NIAGMN_14905 0.47 +1.8 2,975,498 - gph NIAGMN_14905 0.47 +0.1 2,975,586 + gph NIAGMN_14905 0.58 +1.7 2,975,586 + gph NIAGMN_14905 0.58 +0.5 2,975,586 + gph NIAGMN_14905 0.58 +1.2 2,975,587 - gph NIAGMN_14905 0.58 +0.1 2,975,587 - gph NIAGMN_14905 0.58 +0.8 2,975,587 - gph NIAGMN_14905 0.58 -0.3 2,975,587 - gph NIAGMN_14905 0.58 -0.7 2,975,587 - gph NIAGMN_14905 0.58 -1.7 2,975,600 + gph NIAGMN_14905 0.60 +1.7 2,975,732 - gph NIAGMN_14905 0.77 +0.8 2,975,772 + gph NIAGMN_14905 0.83 -0.5 2,975,772 + gph NIAGMN_14905 0.83 -1.2 2,975,778 - gph NIAGMN_14905 0.84 +0.7 2,975,785 + gph NIAGMN_14905 0.84 +0.5 2,975,785 + gph NIAGMN_14905 0.84 +1.1 2,975,785 + gph NIAGMN_14905 0.84 +1.5 2,975,785 + gph NIAGMN_14905 0.84 +0.1 2,975,785 + gph NIAGMN_14905 0.84 +0.1 2,975,785 + gph NIAGMN_14905 0.84 +1.5 2,975,785 + gph NIAGMN_14905 0.84 +0.7 2,975,785 + gph NIAGMN_14905 0.84 -0.7 2,975,785 + gph NIAGMN_14905 0.84 +0.4 2,975,786 - gph NIAGMN_14905 0.85 -0.5 2,975,786 - gph NIAGMN_14905 0.85 +3.1 2,975,786 - gph NIAGMN_14905 0.85 +0.5 2,975,786 - gph NIAGMN_14905 0.85 -0.0 2,975,786 - gph NIAGMN_14905 0.85 +2.1 2,975,786 - gph NIAGMN_14905 0.85 -0.1 2,975,786 - gph NIAGMN_14905 0.85 +0.2 2,975,786 - gph NIAGMN_14905 0.85 -1.5 2,975,786 - gph NIAGMN_14905 0.85 -1.7 2,975,786 - gph NIAGMN_14905 0.85 +2.1 2,975,786 - gph NIAGMN_14905 0.85 +0.2 2,975,786 - gph NIAGMN_14905 0.85 +0.1 2,975,786 - gph NIAGMN_14905 0.85 -0.4 2,975,789 + gph NIAGMN_14905 0.85 +0.3 2,975,789 + gph NIAGMN_14905 0.85 +0.1 2,975,789 + gph NIAGMN_14905 0.85 +0.6 2,975,789 + gph NIAGMN_14905 0.85 -0.2 2,975,800 + gph NIAGMN_14905 0.86 +0.1 2,975,823 + gph NIAGMN_14905 0.89 +1.5 2,975,823 + gph NIAGMN_14905 0.89 -0.6 2,975,828 + +0.1 2,975,828 + -2.0 2,975,836 + -0.4 2,975,838 + -0.3 2,975,838 + -1.8 2,975,838 + +0.5 2,975,838 + -0.9 2,975,838 + -0.5 2,975,838 + -0.5 2,975,838 + +1.1 2,975,859 + -1.1
Or see this region's nucleotide sequence