Strain Fitness in Escherichia coli ECRC102 around NIAGMN_08505

Experiment: Bas14

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntmraY and murF overlap by 7 nucleotidesmurF and murE overlap by 4 nucleotides NIAGMN_08500: mraY - phospho-N-acetylmuramoyl-pentapeptide-transferase , at 1,640,355 to 1,641,437 mraY NIAGMN_08505: murF - UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alani ne ligase, at 1,641,431 to 1,642,789 murF NIAGMN_08510: murE - UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-di aminopimelate ligase, at 1,642,786 to 1,644,273 murE Position (kb) 1641 1642 1643Strain fitness (log2 ratio) -1 0 1at 1641.453 kb on - strandat 1641.462 kb on - strandat 1641.465 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas14
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1,641,453 - +0.6
1,641,462 - +0.0
1,641,465 - -0.2

Or see this region's nucleotide sequence