Strain Fitness in Escherichia coli ECRC102 around NIAGMN_07860

Experiment: Bas14

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyafC and dkgB overlap by 4 nucleotidesdkgB and Asp_trna are separated by 162 nucleotidesAsp_trna and 5S_rrna are separated by 54 nucleotides5S_rrna and 23S_rrna are separated by 94 nucleotides NIAGMN_07850: yafC - DNA-binding transcriptional regulator YafC, at 1,507,834 to 1,508,748 yafC NIAGMN_07855: dkgB - 2,5-didehydrogluconate reductase DkgB, at 1,508,745 to 1,509,548 dkgB NIAGMN_07860: Asp_trna - tRNA-Asp(gtc), at 1,509,711 to 1,509,787 _trna NIAGMN_07865: 5S_rrna - 5S ribosomal RNA, at 1,509,842 to 1,509,957 _rrna NIAGMN_07870: 23S_rrna - 23S ribosomal RNA, at 1,510,052 to 1,512,954 _rrna Position (kb) 1509 1510Strain fitness (log2 ratio) -1 0 1 2at 1508.746 kb on + strandat 1508.746 kb on + strandat 1508.747 kb on - strandat 1508.882 kb on + strand, within dkgBat 1509.017 kb on - strand, within dkgBat 1509.017 kb on - strand, within dkgBat 1509.017 kb on - strand, within dkgBat 1509.023 kb on + strand, within dkgBat 1509.280 kb on + strand, within dkgBat 1509.414 kb on + strand, within dkgBat 1509.548 kb on + strandat 1509.548 kb on + strandat 1509.552 kb on - strandat 1509.569 kb on - strandat 1509.666 kb on - strandat 1509.705 kb on - strandat 1509.708 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas14
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1,508,746 + +0.1
1,508,746 + -0.1
1,508,747 - +1.4
1,508,882 + dkgB NIAGMN_07855 0.17 -0.4
1,509,017 - dkgB NIAGMN_07855 0.34 +1.1
1,509,017 - dkgB NIAGMN_07855 0.34 +1.0
1,509,017 - dkgB NIAGMN_07855 0.34 -0.6
1,509,023 + dkgB NIAGMN_07855 0.35 +1.0
1,509,280 + dkgB NIAGMN_07855 0.67 +0.6
1,509,414 + dkgB NIAGMN_07855 0.83 +0.4
1,509,548 + +2.0
1,509,548 + +0.4
1,509,552 - -0.9
1,509,569 - +0.5
1,509,666 - +2.0
1,509,705 - -0.5
1,509,708 - -0.9

Or see this region's nucleotide sequence