Experiment: Bas14
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pdeB and ybaA are separated by 41 nucleotides ybaA and atl are separated by 378 nucleotides atl and ybaY are separated by 32 nucleotides
NIAGMN_06285: pdeB - cyclic-guanylate-specific phosphodiesterase PdeB, at 1,196,049 to 1,197,599
pdeB
NIAGMN_06290: ybaA - Uncharacterized protein YbaA, at 1,197,641 to 1,197,994
ybaA
NIAGMN_06300: atl - DNA base-flipping protein, at 1,198,373 to 1,198,684
atl
NIAGMN_06305: ybaY - Uncharacterized lipoprotein YbaY, at 1,198,717 to 1,199,289
ybaY
Position (kb) 1197
1198 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 1196.644 kb on + strand, within pdeB at 1196.675 kb on + strand, within pdeB at 1196.688 kb on + strand, within pdeB at 1196.717 kb on + strand, within pdeB at 1196.726 kb on + strand, within pdeB at 1196.910 kb on + strand, within pdeB at 1196.930 kb on - strand, within pdeB at 1197.065 kb on - strand, within pdeB at 1197.065 kb on - strand, within pdeB at 1197.341 kb on - strand, within pdeB at 1197.345 kb on + strand, within pdeB at 1197.346 kb on - strand, within pdeB at 1197.383 kb on + strand, within pdeB at 1197.525 kb on + strand at 1197.643 kb on - strand at 1197.643 kb on - strand at 1197.643 kb on - strand at 1197.673 kb on - strand at 1197.778 kb on - strand, within ybaA at 1197.988 kb on - strand at 1198.002 kb on - strand at 1198.015 kb on - strand at 1198.015 kb on - strand at 1198.436 kb on + strand, within atl at 1198.436 kb on + strand, within atl at 1198.448 kb on + strand, within atl at 1198.510 kb on - strand, within atl at 1198.593 kb on + strand, within atl at 1198.593 kb on + strand, within atl at 1198.659 kb on + strand at 1198.659 kb on + strand at 1198.679 kb on + strand at 1198.679 kb on + strand at 1198.679 kb on + strand at 1198.680 kb on - strand at 1198.680 kb on - strand at 1198.682 kb on + strand at 1198.682 kb on + strand at 1198.711 kb on + strand at 1198.712 kb on - strand at 1198.718 kb on + strand at 1198.718 kb on + strand at 1198.718 kb on + strand at 1198.719 kb on - strand at 1198.721 kb on + strand at 1198.721 kb on + strand at 1198.721 kb on + strand at 1198.722 kb on - strand at 1198.722 kb on - strand at 1198.780 kb on + strand, within ybaY at 1198.786 kb on - strand, within ybaY at 1198.900 kb on - strand, within ybaY at 1198.904 kb on - strand, within ybaY at 1198.945 kb on - strand, within ybaY at 1198.974 kb on + strand, within ybaY at 1198.975 kb on - strand, within ybaY
Per-strain Table
Position Strand Gene LocusTag Fraction Bas14 remove 1,196,644 + pdeB NIAGMN_06285 0.38 +1.9 1,196,675 + pdeB NIAGMN_06285 0.40 +3.1 1,196,688 + pdeB NIAGMN_06285 0.41 +0.3 1,196,717 + pdeB NIAGMN_06285 0.43 +0.9 1,196,726 + pdeB NIAGMN_06285 0.44 +0.3 1,196,910 + pdeB NIAGMN_06285 0.56 -0.7 1,196,930 - pdeB NIAGMN_06285 0.57 +0.9 1,197,065 - pdeB NIAGMN_06285 0.66 -1.3 1,197,065 - pdeB NIAGMN_06285 0.66 -0.0 1,197,341 - pdeB NIAGMN_06285 0.83 +0.3 1,197,345 + pdeB NIAGMN_06285 0.84 -0.1 1,197,346 - pdeB NIAGMN_06285 0.84 -0.1 1,197,383 + pdeB NIAGMN_06285 0.86 +0.9 1,197,525 + -2.4 1,197,643 - -0.1 1,197,643 - +1.2 1,197,643 - -1.1 1,197,673 - -0.5 1,197,778 - ybaA NIAGMN_06290 0.39 -0.1 1,197,988 - +0.5 1,198,002 - -0.3 1,198,015 - -0.5 1,198,015 - -0.7 1,198,436 + atl NIAGMN_06300 0.20 +0.5 1,198,436 + atl NIAGMN_06300 0.20 -1.1 1,198,448 + atl NIAGMN_06300 0.24 -1.6 1,198,510 - atl NIAGMN_06300 0.44 +0.9 1,198,593 + atl NIAGMN_06300 0.71 -0.5 1,198,593 + atl NIAGMN_06300 0.71 +2.9 1,198,659 + +0.9 1,198,659 + -0.1 1,198,679 + -0.2 1,198,679 + +0.6 1,198,679 + +0.2 1,198,680 - +2.5 1,198,680 - +1.0 1,198,682 + +0.3 1,198,682 + +0.2 1,198,711 + -0.5 1,198,712 - -0.9 1,198,718 + -0.1 1,198,718 + -0.6 1,198,718 + -1.1 1,198,719 - -0.2 1,198,721 + +3.9 1,198,721 + -0.5 1,198,721 + -0.2 1,198,722 - +0.9 1,198,722 - +0.1 1,198,780 + ybaY NIAGMN_06305 0.11 -1.5 1,198,786 - ybaY NIAGMN_06305 0.12 -1.1 1,198,900 - ybaY NIAGMN_06305 0.32 +0.9 1,198,904 - ybaY NIAGMN_06305 0.33 +0.5 1,198,945 - ybaY NIAGMN_06305 0.40 -0.2 1,198,974 + ybaY NIAGMN_06305 0.45 +0.9 1,198,975 - ybaY NIAGMN_06305 0.45 -1.1
Or see this region's nucleotide sequence