Strain Fitness in Escherichia coli ECRC102 around NIAGMN_03450

Experiment: Bas14

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntNIAGMN_03445 and NIAGMN_03450 overlap by 4 nucleotidesNIAGMN_03450 and fhaC are separated by 240 nucleotides NIAGMN_03445: NIAGMN_03445 - helicase, at 617,235 to 619,403 _03445 NIAGMN_03450: NIAGMN_03450 - Putative UvrD/REP helicase-like protein, at 619,400 to 619,915 _03450 NIAGMN_03455: fhaC - ShlB/FhaC/HecB family hemolysin secretion/activation protein, at 620,156 to 621,202 fhaC Position (kb) 619 620Strain fitness (log2 ratio) -1 0 1 2at 618.501 kb on - strand, within NIAGMN_03445at 618.878 kb on - strand, within NIAGMN_03445at 619.293 kb on - strandat 620.031 kb on + strandat 620.104 kb on + strandat 620.185 kb on + strandat 620.476 kb on + strand, within fhaCat 620.536 kb on - strand, within fhaCat 620.633 kb on - strand, within fhaCat 620.661 kb on - strand, within fhaCat 620.737 kb on + strand, within fhaCat 620.740 kb on - strand, within fhaCat 620.740 kb on - strand, within fhaCat 620.740 kb on - strand, within fhaCat 620.789 kb on - strand, within fhaCat 620.789 kb on - strand, within fhaCat 620.854 kb on - strand, within fhaCat 620.854 kb on - strand, within fhaC

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas14
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618,501 - NIAGMN_03445 0.58 +1.5
618,878 - NIAGMN_03445 0.76 +2.4
619,293 - +0.1
620,031 + +1.5
620,104 + -0.8
620,185 + -0.9
620,476 + fhaC NIAGMN_03455 0.31 +0.2
620,536 - fhaC NIAGMN_03455 0.36 +1.8
620,633 - fhaC NIAGMN_03455 0.46 -0.5
620,661 - fhaC NIAGMN_03455 0.48 +1.0
620,737 + fhaC NIAGMN_03455 0.55 -0.2
620,740 - fhaC NIAGMN_03455 0.56 +0.5
620,740 - fhaC NIAGMN_03455 0.56 +0.2
620,740 - fhaC NIAGMN_03455 0.56 -1.4
620,789 - fhaC NIAGMN_03455 0.60 +1.2
620,789 - fhaC NIAGMN_03455 0.60 -1.0
620,854 - fhaC NIAGMN_03455 0.67 -0.5
620,854 - fhaC NIAGMN_03455 0.67 +0.1

Or see this region's nucleotide sequence