Experiment: Bas14
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yeeP and NIAGMN_03400 are separated by 328 nucleotides NIAGMN_03400 and NIAGMN_03405 are separated by 213 nucleotides NIAGMN_03405 and NIAGMN_03410 overlap by 4 nucleotides NIAGMN_03410 and NIAGMN_03415 are separated by 53 nucleotides
NIAGMN_03395: yeeP - 50S ribosome-binding GTPase, at 610,758 to 611,630
yeeP
NIAGMN_03400: NIAGMN_03400 - hypothetical protein, at 611,959 to 612,141
_03400
NIAGMN_03405: NIAGMN_03405 - IS66 transposase, at 612,355 to 612,444
_03405
NIAGMN_03410: NIAGMN_03410 - putative transposase of insertion sequence IS682, at 612,441 to 612,866
_03410
NIAGMN_03415: NIAGMN_03415 - Integrase catalytic domain-containing protein, at 612,920 to 613,168
_03415
NIAGMN_03420: NIAGMN_03420 - BfpM-like protein, at 613,050 to 613,550
_03420
Position (kb) 611
612
613 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 611.055 kb on + strand, within yeeP at 611.130 kb on - strand, within yeeP at 611.214 kb on + strand, within yeeP at 611.215 kb on - strand, within yeeP at 611.264 kb on + strand, within yeeP at 611.265 kb on - strand, within yeeP at 611.321 kb on - strand, within yeeP at 611.487 kb on - strand, within yeeP at 611.630 kb on + strand at 611.630 kb on + strand at 611.631 kb on - strand at 611.631 kb on - strand at 611.631 kb on - strand at 611.631 kb on - strand at 611.631 kb on - strand at 611.702 kb on - strand at 611.747 kb on - strand at 611.747 kb on - strand at 611.784 kb on - strand at 611.804 kb on + strand at 612.020 kb on - strand, within NIAGMN_03400 at 612.361 kb on + strand at 612.361 kb on + strand at 612.361 kb on + strand at 612.362 kb on - strand at 612.690 kb on - strand, within NIAGMN_03410 at 612.761 kb on + strand, within NIAGMN_03410 at 612.804 kb on - strand, within NIAGMN_03410 at 612.870 kb on + strand at 612.870 kb on + strand at 612.871 kb on - strand at 612.929 kb on + strand at 612.929 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bas14 remove 611,055 + yeeP NIAGMN_03395 0.34 +0.8 611,130 - yeeP NIAGMN_03395 0.43 +1.8 611,214 + yeeP NIAGMN_03395 0.52 +1.2 611,215 - yeeP NIAGMN_03395 0.52 +0.3 611,264 + yeeP NIAGMN_03395 0.58 -0.0 611,265 - yeeP NIAGMN_03395 0.58 -0.2 611,321 - yeeP NIAGMN_03395 0.64 -0.2 611,487 - yeeP NIAGMN_03395 0.84 +0.1 611,630 + -2.0 611,630 + -1.1 611,631 - -0.2 611,631 - +0.6 611,631 - -0.2 611,631 - -0.2 611,631 - +0.8 611,702 - +0.8 611,747 - +0.2 611,747 - -1.5 611,784 - +4.4 611,804 + -1.0 612,020 - NIAGMN_03400 0.33 -1.0 612,361 + -0.8 612,361 + +0.6 612,361 + +1.2 612,362 - +1.0 612,690 - NIAGMN_03410 0.58 -0.2 612,761 + NIAGMN_03410 0.75 +0.6 612,804 - NIAGMN_03410 0.85 +1.8 612,870 + +1.0 612,870 + -0.5 612,871 - +0.7 612,929 + +0.8 612,929 + +0.9
Or see this region's nucleotide sequence