Experiment: Bas14
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt aat and infA are separated by 284 nucleotides infA and Ser_trna are separated by 253 nucleotides Ser_trna and NIAGMN_03330 are separated by 149 nucleotides NIAGMN_03330 and NIAGMN_03335 are separated by 293 nucleotides NIAGMN_03335 and NIAGMN_03340 overlap by 4 nucleotides
NIAGMN_03315: aat - leucyl/phenylalanyl-tRNA--protein transferase, at 596,283 to 596,987
aat
NIAGMN_03320: infA - translation initiation factor IF-1, at 597,272 to 597,490
infA
NIAGMN_03325: Ser_trna - tRNA-Ser(gga), at 597,744 to 597,831
_trna
NIAGMN_03330: NIAGMN_03330 - restriction endonuclease subunit M, at 597,981 to 598,823
_03330
NIAGMN_03335: NIAGMN_03335 - DUF5983 domain-containing protein, at 599,117 to 599,608
_03335
NIAGMN_03340: NIAGMN_03340 - toxin, at 599,605 to 599,979
_03340
Position (kb) 597
598
599 Strain fitness (log2 ratio) -2
-1
0
1
2 at 597.020 kb on + strand at 597.118 kb on + strand at 597.118 kb on + strand at 597.118 kb on + strand at 597.118 kb on + strand at 597.118 kb on + strand at 597.119 kb on - strand at 597.119 kb on - strand at 597.528 kb on + strand at 597.588 kb on + strand at 597.589 kb on - strand at 597.615 kb on + strand at 597.616 kb on - strand at 597.695 kb on + strand at 597.697 kb on + strand at 597.697 kb on + strand at 597.837 kb on + strand at 597.870 kb on + strand at 597.921 kb on + strand at 597.922 kb on - strand at 597.924 kb on - strand at 598.112 kb on - strand, within NIAGMN_03330 at 598.395 kb on - strand, within NIAGMN_03330 at 598.454 kb on + strand, within NIAGMN_03330 at 598.482 kb on - strand, within NIAGMN_03330 at 598.653 kb on - strand, within NIAGMN_03330 at 598.724 kb on + strand, within NIAGMN_03330 at 598.847 kb on - strand at 598.847 kb on - strand at 599.713 kb on + strand, within NIAGMN_03340
Per-strain Table
Position Strand Gene LocusTag Fraction Bas14 remove 597,020 + -0.2 597,118 + -1.0 597,118 + +0.6 597,118 + -0.1 597,118 + +1.0 597,118 + +0.5 597,119 - +2.5 597,119 - -0.5 597,528 + -1.6 597,588 + +0.2 597,589 - +0.2 597,615 + +0.0 597,616 - +0.8 597,695 + +0.8 597,697 + -0.1 597,697 + -0.8 597,837 + -0.2 597,870 + -0.7 597,921 + -1.1 597,922 - -1.6 597,924 - +0.8 598,112 - NIAGMN_03330 0.16 +0.4 598,395 - NIAGMN_03330 0.49 -1.8 598,454 + NIAGMN_03330 0.56 +0.8 598,482 - NIAGMN_03330 0.59 +1.3 598,653 - NIAGMN_03330 0.80 +0.2 598,724 + NIAGMN_03330 0.88 +0.2 598,847 - -0.6 598,847 - +0.8 599,713 + NIAGMN_03340 0.29 -0.1
Or see this region's nucleotide sequence