Strain Fitness in Escherichia coli ECRC102 around NIAGMN_03015

Experiment: Bas14

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntycbX and zapC overlap by 4 nucleotideszapC and pyrD are separated by 173 nucleotidespyrD and NIAGMN_03020 are separated by 277 nucleotidesNIAGMN_03020 and fimA are separated by 13 nucleotides NIAGMN_03005: ycbX - 6-N-hydroxylaminopurine resistance protein YcbX, at 517,721 to 518,830 ycbX NIAGMN_03010: zapC - cell division protein ZapC, at 518,827 to 519,369 zapC NIAGMN_03015: pyrD - quinone-dependent dihydroorotate dehydrogenase, at 519,543 to 520,553 pyrD NIAGMN_03020: NIAGMN_03020 - PapD-N domain-containing protein, at 520,831 to 521,352 _03020 NIAGMN_03025: fimA - fimbrial protein, at 521,366 to 521,929 fimA Position (kb) 519 520 521Strain fitness (log2 ratio) -1 0 1 2 3at 518.895 kb on + strand, within zapCat 518.959 kb on + strand, within zapCat 519.200 kb on + strand, within zapCat 519.289 kb on - strand, within zapCat 519.429 kb on - strandat 519.461 kb on + strandat 519.514 kb on - strandat 519.519 kb on - strandat 519.816 kb on - strand, within pyrDat 519.853 kb on - strand, within pyrDat 520.023 kb on - strand, within pyrDat 520.183 kb on - strand, within pyrDat 520.413 kb on - strand, within pyrDat 520.532 kb on + strandat 520.546 kb on + strandat 520.549 kb on + strandat 520.549 kb on + strandat 520.742 kb on + strandat 520.798 kb on + strandat 520.818 kb on - strandat 520.842 kb on + strandat 520.842 kb on + strandat 520.843 kb on - strandat 520.845 kb on + strandat 520.845 kb on + strandat 520.954 kb on - strand, within NIAGMN_03020at 520.979 kb on - strand, within NIAGMN_03020at 521.024 kb on - strand, within NIAGMN_03020at 521.239 kb on - strand, within NIAGMN_03020at 521.268 kb on + strand, within NIAGMN_03020at 521.364 kb on + strandat 521.364 kb on + strandat 521.365 kb on - strandat 521.373 kb on + strandat 521.373 kb on + strandat 521.381 kb on + strandat 521.382 kb on - strandat 521.474 kb on - strand, within fimAat 521.477 kb on + strand, within fimAat 521.477 kb on + strand, within fimAat 521.478 kb on - strand, within fimA

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas14
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518,895 + zapC NIAGMN_03010 0.13 -0.9
518,959 + zapC NIAGMN_03010 0.24 +2.7
519,200 + zapC NIAGMN_03010 0.69 +1.3
519,289 - zapC NIAGMN_03010 0.85 -0.3
519,429 - +1.7
519,461 + -0.5
519,514 - -0.3
519,519 - +0.1
519,816 - pyrD NIAGMN_03015 0.27 -0.9
519,853 - pyrD NIAGMN_03015 0.31 -0.9
520,023 - pyrD NIAGMN_03015 0.47 -1.2
520,183 - pyrD NIAGMN_03015 0.63 +0.7
520,413 - pyrD NIAGMN_03015 0.86 -0.6
520,532 + +3.7
520,546 + +1.5
520,549 + -0.5
520,549 + -0.6
520,742 + +1.7
520,798 + +0.7
520,818 - +1.7
520,842 + +2.1
520,842 + -0.9
520,843 - -0.5
520,845 + -0.6
520,845 + +1.0
520,954 - NIAGMN_03020 0.24 +0.5
520,979 - NIAGMN_03020 0.28 -0.9
521,024 - NIAGMN_03020 0.37 -0.6
521,239 - NIAGMN_03020 0.78 +0.1
521,268 + NIAGMN_03020 0.84 +3.3
521,364 + +0.1
521,364 + +0.0
521,365 - +1.3
521,373 + +0.7
521,373 + +1.7
521,381 + -0.5
521,382 - +1.1
521,474 - fimA NIAGMN_03025 0.19 -1.6
521,477 + fimA NIAGMN_03025 0.20 -0.9
521,477 + fimA NIAGMN_03025 0.20 +0.7
521,478 - fimA NIAGMN_03025 0.20 -0.6

Or see this region's nucleotide sequence