Experiment: Bas14
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt NIAGMN_02810 and NIAGMN_02815 are separated by 206 nucleotides NIAGMN_02815 and NIAGMN_02820 overlap by 17 nucleotides NIAGMN_02820 and hipB are separated by 108 nucleotides hipB and NIAGMN_02830 are separated by 27 nucleotides
NIAGMN_02810: NIAGMN_02810 - hypothetical protein, at 484,978 to 485,433
_02810
NIAGMN_02815: NIAGMN_02815 - Rha family transcriptional regulator, at 485,640 to 486,065
_02815
NIAGMN_02820: NIAGMN_02820 - Putative antitoxin of bacterial toxin-antitoxin system, YdaS/YdaT, at 486,049 to 486,321
_02820
NIAGMN_02825: hipB - XRE family transcriptional regulator, at 486,430 to 486,831
hipB
NIAGMN_02830: NIAGMN_02830 - antitoxin, at 486,859 to 487,050
_02830
Position (kb) 486
487 Strain fitness (log2 ratio) -1
0
1
2
3 at 485.431 kb on + strand at 485.528 kb on + strand at 486.138 kb on + strand, within NIAGMN_02820 at 486.312 kb on + strand at 486.853 kb on + strand at 487.031 kb on + strand at 487.031 kb on + strand at 487.035 kb on + strand at 487.103 kb on - strand at 487.139 kb on + strand at 487.139 kb on + strand at 487.171 kb on + strand at 487.192 kb on - strand at 487.195 kb on + strand at 487.196 kb on - strand at 487.197 kb on + strand at 487.247 kb on + strand at 487.251 kb on + strand at 487.251 kb on + strand at 487.255 kb on + strand at 487.270 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bas14 remove 485,431 + +0.9 485,528 + +1.2 486,138 + NIAGMN_02820 0.33 -0.4 486,312 + -0.9 486,853 + +3.3 487,031 + -0.2 487,031 + -1.5 487,035 + +0.6 487,103 - +0.8 487,139 + -1.1 487,139 + +0.3 487,171 + +0.3 487,192 - -0.6 487,195 + +2.5 487,196 - +0.2 487,197 + +0.8 487,247 + +1.8 487,251 + +1.8 487,251 + +1.6 487,255 + +0.8 487,270 + +0.2
Or see this region's nucleotide sequence