Experiment: Bas14
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Leu_trna and Cys_trna are separated by 12 nucleotides Cys_trna and Gly_trna are separated by 53 nucleotides Gly_trna and pgsA are separated by 151 nucleotides pgsA and uvrC are separated by 56 nucleotides uvrC and uvrC overlap by 4 nucleotides
NIAGMN_02205: Leu_trna - tRNA-Leu(taa), at 389,675 to 389,761
_trna
NIAGMN_02210: Cys_trna - tRNA-Cys(gca), at 389,774 to 389,847
_trna
NIAGMN_02215: Gly_trna - tRNA-Gly(gcc), at 389,901 to 389,976
_trna
NIAGMN_02220: pgsA - CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase, at 390,128 to 390,676
pgsA
NIAGMN_02225: uvrC - excinuclease ABC subunit UvrC, at 390,733 to 392,565
uvrC
NIAGMN_02230: uvrC - excinuclease ABC subunit UvrC, at 392,562 to 393,218
uvrC
Position (kb) 390
391
392
393 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 389.996 kb on - strand at 390.842 kb on + strand at 390.843 kb on - strand at 390.979 kb on - strand, within uvrC at 391.174 kb on - strand, within uvrC at 391.194 kb on + strand, within uvrC at 391.360 kb on - strand, within uvrC at 391.763 kb on - strand, within uvrC at 391.986 kb on - strand, within uvrC at 391.986 kb on - strand, within uvrC at 392.054 kb on - strand, within uvrC at 392.054 kb on - strand, within uvrC at 392.254 kb on - strand, within uvrC at 392.316 kb on - strand, within uvrC at 392.316 kb on - strand, within uvrC at 392.316 kb on - strand, within uvrC at 392.362 kb on + strand, within uvrC at 392.363 kb on - strand, within uvrC at 392.472 kb on - strand at 392.472 kb on - strand at 392.472 kb on - strand at 392.476 kb on - strand at 392.496 kb on - strand at 392.496 kb on - strand at 392.575 kb on - strand at 392.748 kb on - strand, within uvrC at 392.815 kb on - strand, within uvrC at 392.848 kb on + strand, within uvrC at 393.148 kb on - strand, within uvrC at 393.148 kb on - strand, within uvrC at 393.150 kb on - strand, within uvrC at 393.204 kb on + strand at 393.236 kb on + strand at 393.255 kb on - strand at 393.255 kb on - strand at 393.255 kb on - strand at 393.259 kb on - strand at 393.359 kb on + strand at 393.474 kb on + strand at 393.480 kb on + strand at 393.538 kb on + strand at 393.538 kb on + strand at 393.541 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bas14 remove 389,996 - +1.7 390,842 + -0.5 390,843 - +1.7 390,979 - uvrC NIAGMN_02225 0.13 +3.3 391,174 - uvrC NIAGMN_02225 0.24 +0.7 391,194 + uvrC NIAGMN_02225 0.25 -0.3 391,360 - uvrC NIAGMN_02225 0.34 +0.1 391,763 - uvrC NIAGMN_02225 0.56 -0.9 391,986 - uvrC NIAGMN_02225 0.68 +0.7 391,986 - uvrC NIAGMN_02225 0.68 -2.0 392,054 - uvrC NIAGMN_02225 0.72 +0.1 392,054 - uvrC NIAGMN_02225 0.72 -0.0 392,254 - uvrC NIAGMN_02225 0.83 +1.1 392,316 - uvrC NIAGMN_02225 0.86 +0.1 392,316 - uvrC NIAGMN_02225 0.86 -0.8 392,316 - uvrC NIAGMN_02225 0.86 -1.3 392,362 + uvrC NIAGMN_02225 0.89 +0.6 392,363 - uvrC NIAGMN_02225 0.89 +1.8 392,472 - +1.4 392,472 - +0.8 392,472 - +0.9 392,476 - +0.8 392,496 - +0.6 392,496 - -1.4 392,575 - +0.4 392,748 - uvrC NIAGMN_02230 0.28 +3.3 392,815 - uvrC NIAGMN_02230 0.39 +0.8 392,848 + uvrC NIAGMN_02230 0.44 -0.1 393,148 - uvrC NIAGMN_02230 0.89 +2.8 393,148 - uvrC NIAGMN_02230 0.89 -0.6 393,150 - uvrC NIAGMN_02230 0.89 -0.6 393,204 + -0.4 393,236 + -1.1 393,255 - -0.6 393,255 - +1.0 393,255 - +0.9 393,259 - +1.3 393,359 + +1.3 393,474 + -1.4 393,480 + +0.4 393,538 + +0.4 393,538 + +0.5 393,541 - +0.8
Or see this region's nucleotide sequence