Experiment: Bas14
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yecD and yecE overlap by 4 nucleotides yecE and yecN are separated by 52 nucleotides yecN and cmoA are separated by 40 nucleotides cmoA and cmoB overlap by 4 nucleotides
NIAGMN_01820: yecD - hydrolase, at 328,344 to 328,910
yecD
NIAGMN_01825: yecE - UPF0759 protein YecE, at 328,907 to 329,725
yecE
NIAGMN_01830: yecN - Inner membrane protein YecN, at 329,778 to 330,173
yecN
NIAGMN_01835: cmoA - carboxy-S-adenosyl-L-methionine synthase CmoA, at 330,214 to 330,957
cmoA
NIAGMN_01840: cmoB - tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic acid(34) synthase CmoB, at 330,954 to 331,925
cmoB
Position (kb) 329
330
331 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 328.785 kb on - strand, within yecD at 328.838 kb on + strand, within yecD at 328.845 kb on - strand, within yecD at 328.853 kb on + strand, within yecD at 328.980 kb on - strand at 329.286 kb on - strand, within yecE at 329.475 kb on + strand, within yecE at 329.605 kb on - strand, within yecE at 329.609 kb on - strand, within yecE at 329.618 kb on - strand, within yecE at 329.644 kb on + strand at 329.645 kb on - strand at 329.661 kb on + strand at 329.662 kb on - strand at 329.666 kb on - strand at 329.736 kb on + strand at 329.736 kb on + strand at 329.737 kb on - strand at 329.737 kb on - strand at 329.737 kb on - strand at 329.934 kb on + strand, within yecN at 329.934 kb on + strand, within yecN at 329.935 kb on - strand, within yecN at 329.936 kb on + strand, within yecN at 330.141 kb on - strand at 330.141 kb on - strand at 330.141 kb on - strand at 330.339 kb on + strand, within cmoA at 330.565 kb on - strand, within cmoA at 331.136 kb on + strand, within cmoB
Per-strain Table
Position Strand Gene LocusTag Fraction Bas14 remove 328,785 - yecD NIAGMN_01820 0.78 +0.5 328,838 + yecD NIAGMN_01820 0.87 -1.3 328,845 - yecD NIAGMN_01820 0.88 -0.1 328,853 + yecD NIAGMN_01820 0.90 +2.7 328,980 - -0.6 329,286 - yecE NIAGMN_01825 0.46 -0.6 329,475 + yecE NIAGMN_01825 0.69 -0.9 329,605 - yecE NIAGMN_01825 0.85 +0.9 329,609 - yecE NIAGMN_01825 0.86 -1.1 329,618 - yecE NIAGMN_01825 0.87 -1.6 329,644 + +0.7 329,645 - +0.1 329,661 + +0.3 329,662 - +1.2 329,666 - +0.8 329,736 + +0.7 329,736 + +0.3 329,737 - +2.7 329,737 - +1.7 329,737 - +0.7 329,934 + yecN NIAGMN_01830 0.39 +0.1 329,934 + yecN NIAGMN_01830 0.39 +0.7 329,935 - yecN NIAGMN_01830 0.40 -2.0 329,936 + yecN NIAGMN_01830 0.40 +1.7 330,141 - +3.0 330,141 - -0.6 330,141 - +0.0 330,339 + cmoA NIAGMN_01835 0.17 +1.5 330,565 - cmoA NIAGMN_01835 0.47 +0.7 331,136 + cmoB NIAGMN_01840 0.19 -0.9
Or see this region's nucleotide sequence