Experiment: Bas14
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt manZ and NIAGMN_01545 are separated by 54 nucleotides NIAGMN_01545 and NIAGMN_01550 are separated by 310 nucleotides NIAGMN_01550 and mntP overlap by 4 nucleotides mntP and rlmA overlap by 4 nucleotides rlmA and cspE are separated by 165 nucleotides
NIAGMN_01540: manZ - PTS mannose transporter subunit IID, at 281,306 to 282,157
manZ
NIAGMN_01545: NIAGMN_01545 - UPF0266 membrane protein YobD, at 282,212 to 282,670
_01545
NIAGMN_01550: NIAGMN_01550 - Membrane protein, at 282,981 to 283,103
_01550
NIAGMN_01555: mntP - manganese efflux pump MntP, at 283,100 to 283,666
mntP
NIAGMN_01560: rlmA - 23S rRNA (guanine(745)-N(1))-methyltransferase, at 283,663 to 284,472
rlmA
NIAGMN_01565: cspE - transcription antiterminator/RNA stability regulator CspE, at 284,638 to 284,847
cspE
Position (kb) 283
284 Strain fitness (log2 ratio) -2
-1
0
1 at 282.166 kb on + strand at 282.166 kb on + strand at 282.980 kb on + strand at 283.041 kb on + strand, within NIAGMN_01550 at 283.041 kb on + strand, within NIAGMN_01550 at 283.041 kb on + strand, within NIAGMN_01550 at 283.041 kb on + strand, within NIAGMN_01550 at 283.042 kb on - strand, within NIAGMN_01550 at 283.135 kb on + strand at 283.537 kb on + strand, within mntP at 283.537 kb on + strand, within mntP at 283.668 kb on - strand at 283.888 kb on + strand, within rlmA at 284.007 kb on - strand, within rlmA at 284.029 kb on - strand, within rlmA at 284.199 kb on + strand, within rlmA at 284.415 kb on + strand at 284.417 kb on + strand at 284.611 kb on - strand at 284.644 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bas14 remove 282,166 + -0.3 282,166 + +0.1 282,980 + +1.1 283,041 + NIAGMN_01550 0.49 -0.6 283,041 + NIAGMN_01550 0.49 -2.2 283,041 + NIAGMN_01550 0.49 -1.1 283,041 + NIAGMN_01550 0.49 -2.3 283,042 - NIAGMN_01550 0.50 -0.5 283,135 + +0.7 283,537 + mntP NIAGMN_01555 0.77 -0.0 283,537 + mntP NIAGMN_01555 0.77 -0.7 283,668 - -0.3 283,888 + rlmA NIAGMN_01560 0.28 -1.8 284,007 - rlmA NIAGMN_01560 0.42 -1.4 284,029 - rlmA NIAGMN_01560 0.45 -0.1 284,199 + rlmA NIAGMN_01560 0.66 +0.3 284,415 + -0.6 284,417 + +0.7 284,611 - -0.3 284,644 - -0.2
Or see this region's nucleotide sequence