Experiment: Bas14
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt btuD and btuE overlap by 1 nucleotides btuE and btuC are separated by 62 nucleotides btuC and NIAGMN_00975 are separated by 100 nucleotides NIAGMN_00975 and pheT are separated by 4 nucleotides
NIAGMN_00955: btuD - vitamin B12 ABC transporter ATP-binding protein BtuD, at 168,094 to 168,843
btuD
NIAGMN_00960: btuE - bifunctional thioredoxin/glutathione peroxidase, at 168,843 to 169,394
btuE
NIAGMN_00965: btuC - vitamin B12 ABC transporter permease BtuC, at 169,457 to 170,437
btuC
NIAGMN_00975: NIAGMN_00975 - Integration host factor subunit alpha, at 170,538 to 170,837
_00975
NIAGMN_00980: pheT - phenylalanine--tRNA ligase subunit beta, at 170,842 to 173,229
pheT
Position (kb) 169
170
171 Strain fitness (log2 ratio) -1
0
1
2 at 168.527 kb on - strand, within btuD at 168.730 kb on - strand, within btuD at 169.526 kb on - strand at 169.651 kb on + strand, within btuC at 169.673 kb on - strand, within btuC at 169.761 kb on - strand, within btuC at 169.975 kb on + strand, within btuC at 170.441 kb on - strand at 170.526 kb on - strand at 170.530 kb on - strand at 170.670 kb on - strand, within NIAGMN_00975 at 170.670 kb on - strand, within NIAGMN_00975 at 170.688 kb on - strand, within NIAGMN_00975 at 170.784 kb on - strand, within NIAGMN_00975
Per-strain Table
Position Strand Gene LocusTag Fraction Bas14 remove 168,527 - btuD NIAGMN_00955 0.58 -1.6 168,730 - btuD NIAGMN_00955 0.85 -1.1 169,526 - +1.0 169,651 + btuC NIAGMN_00965 0.20 -0.2 169,673 - btuC NIAGMN_00965 0.22 +1.0 169,761 - btuC NIAGMN_00965 0.31 +0.7 169,975 + btuC NIAGMN_00965 0.53 +1.7 170,441 - +1.2 170,526 - +0.3 170,530 - +0.5 170,670 - NIAGMN_00975 0.44 +0.9 170,670 - NIAGMN_00975 0.44 +2.3 170,688 - NIAGMN_00975 0.50 +0.7 170,784 - NIAGMN_00975 0.82 +0.4
Or see this region's nucleotide sequence