Strain Fitness in Escherichia coli ECRC102 around NIAGMN_00705

Experiment: Bas14

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntcfa and ribE are separated by 39 nucleotidesribE and mdtK are separated by 214 nucleotidesmdtK and ydhQ are separated by 40 nucleotides NIAGMN_00695: cfa - cyclopropane fatty acyl phospholipid synthase, at 116,695 to 117,843 cfa NIAGMN_00700: ribE - riboflavin synthase, at 117,883 to 118,524 ribE NIAGMN_00705: mdtK - multidrug efflux MATE transporter MdtK, at 118,739 to 120,112 mdtK NIAGMN_00710: ydhQ - Uncharacterized protein YdhQ, at 120,153 to 121,409 ydhQ Position (kb) 118 119 120 121Strain fitness (log2 ratio) -2 -1 0 1 2 3at 117.739 kb on + strandat 117.848 kb on + strandat 118.715 kb on + strandat 118.715 kb on + strandat 118.726 kb on - strandat 118.750 kb on + strandat 118.751 kb on - strandat 118.780 kb on - strandat 119.586 kb on + strand, within mdtKat 119.881 kb on + strand, within mdtKat 119.903 kb on + strand, within mdtKat 120.006 kb on + strandat 120.252 kb on + strandat 120.531 kb on - strand, within ydhQat 120.792 kb on - strand, within ydhQ

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas14
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117,739 + +0.7
117,848 + +1.1
118,715 + +1.7
118,715 + -1.5
118,726 - +0.7
118,750 + -0.5
118,751 - -2.2
118,780 - +3.7
119,586 + mdtK NIAGMN_00705 0.62 -1.3
119,881 + mdtK NIAGMN_00705 0.83 +0.7
119,903 + mdtK NIAGMN_00705 0.85 +2.0
120,006 + -0.5
120,252 + +1.3
120,531 - ydhQ NIAGMN_00710 0.30 +1.7
120,792 - ydhQ NIAGMN_00710 0.51 -0.4

Or see this region's nucleotide sequence