Strain Fitness in Hydrogenophaga sp. GW460-11-11-14-LB1 around GFF5309

Experiment: Ying_all64 rep A; time point 2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF5305 and GFF5306 are separated by 46 nucleotidesGFF5306 and GFF5307 are separated by 44 nucleotidesGFF5307 and GFF5308 overlap by 4 nucleotidesGFF5308 and GFF5309 overlap by 4 nucleotidesGFF5309 and GFF5310 overlap by 4 nucleotides GFF5305 - gene 66 protein, at 2,316,627 to 2,317,052 GFF5305 GFF5306 - hypothetical protein, at 2,317,099 to 2,317,473 GFF5306 GFF5307 - hypothetical protein, at 2,317,518 to 2,317,730 GFF5307 GFF5308 - hypothetical protein, at 2,317,727 to 2,317,963 GFF5308 GFF5309 - hypothetical protein, at 2,317,960 to 2,318,475 GFF5309 GFF5310 - Replicative DNA helicase (EC 3.6.1.-), at 2,318,472 to 2,319,896 GFF5310 Position (kb) 2317 2318 2319Strain fitness (log2 ratio) -1 0 1 2at 2317.461 kb on - strandat 2317.502 kb on - strandat 2317.782 kb on + strand, within GFF5308at 2317.783 kb on - strand, within GFF5308at 2318.051 kb on + strand, within GFF5309at 2318.153 kb on + strand, within GFF5309at 2318.154 kb on - strand, within GFF5309at 2318.560 kb on + strandat 2318.561 kb on - strandat 2318.561 kb on - strandat 2318.561 kb on - strandat 2318.624 kb on - strand, within GFF5310at 2318.669 kb on - strand, within GFF5310at 2318.833 kb on - strand, within GFF5310at 2318.893 kb on + strand, within GFF5310at 2318.962 kb on - strand, within GFF5310

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_all64 rep A; time point 2
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2,317,461 - -0.2
2,317,502 - +0.4
2,317,782 + GFF5308 0.23 -0.6
2,317,783 - GFF5308 0.24 -0.1
2,318,051 + GFF5309 0.18 -0.3
2,318,153 + GFF5309 0.37 +1.9
2,318,154 - GFF5309 0.38 +0.4
2,318,560 + -0.2
2,318,561 - +0.4
2,318,561 - +0.0
2,318,561 - +0.4
2,318,624 - GFF5310 0.11 -0.1
2,318,669 - GFF5310 0.14 +0.1
2,318,833 - GFF5310 0.25 -0.1
2,318,893 + GFF5310 0.30 +0.4
2,318,962 - GFF5310 0.34 +0.3

Or see this region's nucleotide sequence