Experiment: Ying_all64 rep A; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4460 and GFF4461 overlap by 4 nucleotides GFF4461 and GFF4462 overlap by 1 nucleotides GFF4462 and GFF4463 overlap by 4 nucleotides GFF4463 and GFF4464 are separated by 95 nucleotides
GFF4460 - Anti-sigma F factor antagonist (spoIIAA-2); Anti-sigma B factor antagonist RsbV, at 1,388,189 to 1,388,533
GFF4460
GFF4461 - UPF0028 protein YchK, at 1,388,530 to 1,389,417
GFF4461
GFF4462 - Serine phosphatase RsbU, regulator of sigma subunit, at 1,389,417 to 1,390,625
GFF4462
GFF4463 - Acetyl-CoA synthetase (ADP-forming) alpha and beta chains, putative, at 1,390,622 to 1,391,125
GFF4463
GFF4464 - Methyltransferase FkbM, at 1,391,221 to 1,391,976
GFF4464
Position (kb)
1389
1390
1391 Strain fitness (log2 ratio)
-1
0
1
2 at 1388.487 kb on - strand, within GFF4460 at 1388.487 kb on - strand, within GFF4460 at 1389.058 kb on + strand, within GFF4461 at 1389.058 kb on + strand, within GFF4461 at 1389.317 kb on - strand, within GFF4461 at 1389.317 kb on - strand, within GFF4461 at 1389.361 kb on + strand at 1389.987 kb on + strand, within GFF4462 at 1389.996 kb on + strand, within GFF4462 at 1389.996 kb on + strand, within GFF4462 at 1389.997 kb on - strand, within GFF4462 at 1389.997 kb on - strand, within GFF4462 at 1389.997 kb on - strand, within GFF4462 at 1390.173 kb on + strand, within GFF4462 at 1390.285 kb on - strand, within GFF4462 at 1390.414 kb on - strand, within GFF4462 at 1390.775 kb on + strand, within GFF4463 at 1390.776 kb on - strand, within GFF4463 at 1390.776 kb on - strand, within GFF4463 at 1390.776 kb on - strand, within GFF4463 at 1391.023 kb on + strand, within GFF4463 at 1391.151 kb on + strand at 1391.151 kb on + strand at 1391.152 kb on - strand at 1391.152 kb on - strand at 1391.152 kb on - strand at 1391.311 kb on + strand, within GFF4464
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_all64 rep A; time point 2 remove 1,388,487 - GFF4460 0.86 +1.1 1,388,487 - GFF4460 0.86 +0.3 1,389,058 + GFF4461 0.59 +0.3 1,389,058 + GFF4461 0.59 +0.9 1,389,317 - GFF4461 0.89 +1.3 1,389,317 - GFF4461 0.89 +1.0 1,389,361 + +0.9 1,389,987 + GFF4462 0.47 +0.1 1,389,996 + GFF4462 0.48 +0.1 1,389,996 + GFF4462 0.48 +1.0 1,389,997 - GFF4462 0.48 -1.6 1,389,997 - GFF4462 0.48 -0.5 1,389,997 - GFF4462 0.48 -0.3 1,390,173 + GFF4462 0.63 +0.1 1,390,285 - GFF4462 0.72 +2.0 1,390,414 - GFF4462 0.82 -0.6 1,390,775 + GFF4463 0.30 +0.0 1,390,776 - GFF4463 0.31 -0.1 1,390,776 - GFF4463 0.31 -1.3 1,390,776 - GFF4463 0.31 +0.5 1,391,023 + GFF4463 0.80 +0.1 1,391,151 + +0.3 1,391,151 + -0.4 1,391,152 - -0.7 1,391,152 - -0.9 1,391,152 - -0.5 1,391,311 + GFF4464 0.12 -0.2
Or see this region's nucleotide sequence