Strain Fitness in Hydrogenophaga sp. GW460-11-11-14-LB1 around GFF4462

Experiment: Ying_all64 rep A; time point 2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF4460 and GFF4461 overlap by 4 nucleotidesGFF4461 and GFF4462 overlap by 1 nucleotidesGFF4462 and GFF4463 overlap by 4 nucleotidesGFF4463 and GFF4464 are separated by 95 nucleotides GFF4460 - Anti-sigma F factor antagonist (spoIIAA-2); Anti-sigma B factor antagonist RsbV, at 1,388,189 to 1,388,533 GFF4460 GFF4461 - UPF0028 protein YchK, at 1,388,530 to 1,389,417 GFF4461 GFF4462 - Serine phosphatase RsbU, regulator of sigma subunit, at 1,389,417 to 1,390,625 GFF4462 GFF4463 - Acetyl-CoA synthetase (ADP-forming) alpha and beta chains, putative, at 1,390,622 to 1,391,125 GFF4463 GFF4464 - Methyltransferase FkbM, at 1,391,221 to 1,391,976 GFF4464 Position (kb) 1389 1390 1391Strain fitness (log2 ratio) -1 0 1 2at 1388.487 kb on - strand, within GFF4460at 1388.487 kb on - strand, within GFF4460at 1389.058 kb on + strand, within GFF4461at 1389.058 kb on + strand, within GFF4461at 1389.317 kb on - strand, within GFF4461at 1389.317 kb on - strand, within GFF4461at 1389.361 kb on + strandat 1389.987 kb on + strand, within GFF4462at 1389.996 kb on + strand, within GFF4462at 1389.996 kb on + strand, within GFF4462at 1389.997 kb on - strand, within GFF4462at 1389.997 kb on - strand, within GFF4462at 1389.997 kb on - strand, within GFF4462at 1390.173 kb on + strand, within GFF4462at 1390.285 kb on - strand, within GFF4462at 1390.414 kb on - strand, within GFF4462at 1390.775 kb on + strand, within GFF4463at 1390.776 kb on - strand, within GFF4463at 1390.776 kb on - strand, within GFF4463at 1390.776 kb on - strand, within GFF4463at 1391.023 kb on + strand, within GFF4463at 1391.151 kb on + strandat 1391.151 kb on + strandat 1391.152 kb on - strandat 1391.152 kb on - strandat 1391.152 kb on - strandat 1391.311 kb on + strand, within GFF4464

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_all64 rep A; time point 2
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1,388,487 - GFF4460 0.86 +1.1
1,388,487 - GFF4460 0.86 +0.3
1,389,058 + GFF4461 0.59 +0.3
1,389,058 + GFF4461 0.59 +0.9
1,389,317 - GFF4461 0.89 +1.3
1,389,317 - GFF4461 0.89 +1.0
1,389,361 + +0.9
1,389,987 + GFF4462 0.47 +0.1
1,389,996 + GFF4462 0.48 +0.1
1,389,996 + GFF4462 0.48 +1.0
1,389,997 - GFF4462 0.48 -1.6
1,389,997 - GFF4462 0.48 -0.5
1,389,997 - GFF4462 0.48 -0.3
1,390,173 + GFF4462 0.63 +0.1
1,390,285 - GFF4462 0.72 +2.0
1,390,414 - GFF4462 0.82 -0.6
1,390,775 + GFF4463 0.30 +0.0
1,390,776 - GFF4463 0.31 -0.1
1,390,776 - GFF4463 0.31 -1.3
1,390,776 - GFF4463 0.31 +0.5
1,391,023 + GFF4463 0.80 +0.1
1,391,151 + +0.3
1,391,151 + -0.4
1,391,152 - -0.7
1,391,152 - -0.9
1,391,152 - -0.5
1,391,311 + GFF4464 0.12 -0.2

Or see this region's nucleotide sequence