Experiment: Ying_all64 rep A; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3145 and GFF3146 overlap by 4 nucleotides GFF3146 and GFF3147 overlap by 14 nucleotides GFF3147 and GFF3148 overlap by 4 nucleotides GFF3148 and GFF3149 overlap by 14 nucleotides
GFF3145 - Conjugative transfer protein TraV, at 23,883 to 24,620
GFF3145
GFF3146 - Thiol:disulfide involved in conjugative transfer, at 24,617 to 25,486
GFF3146
GFF3147 - IncF plasmid conjugative transfer pilus assembly protein TraB, at 25,473 to 26,891
GFF3147
GFF3148 - IncF plasmid conjugative transfer pilus assembly protein TraK, at 26,888 to 27,883
GFF3148
GFF3149 - IncF plasmid conjugative transfer pilus assembly protein TraE, at 27,870 to 28,436
GFF3149
Position (kb)
25
26
27 Strain fitness (log2 ratio)
-1
0
1 at 24.514 kb on - strand, within GFF3145 at 24.564 kb on - strand at 24.842 kb on + strand, within GFF3146 at 24.933 kb on + strand, within GFF3146 at 25.079 kb on + strand, within GFF3146 at 25.228 kb on - strand, within GFF3146 at 25.228 kb on - strand, within GFF3146 at 25.258 kb on - strand, within GFF3146 at 25.258 kb on - strand, within GFF3146 at 25.477 kb on + strand at 25.478 kb on - strand at 25.478 kb on - strand at 25.657 kb on + strand, within GFF3147 at 25.658 kb on - strand, within GFF3147 at 25.660 kb on + strand, within GFF3147 at 25.661 kb on - strand, within GFF3147 at 25.661 kb on - strand, within GFF3147 at 25.768 kb on + strand, within GFF3147 at 26.916 kb on + strand at 26.916 kb on + strand at 26.917 kb on - strand at 26.917 kb on - strand at 26.917 kb on - strand at 27.337 kb on - strand, within GFF3148 at 27.337 kb on - strand, within GFF3148 at 27.402 kb on + strand, within GFF3148 at 27.402 kb on + strand, within GFF3148 at 27.403 kb on - strand, within GFF3148 at 27.403 kb on - strand, within GFF3148
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_all64 rep A; time point 2 remove 24,514 - GFF3145 0.86 -0.7 24,564 - -0.3 24,842 + GFF3146 0.26 +1.3 24,933 + GFF3146 0.36 -0.1 25,079 + GFF3146 0.53 -0.8 25,228 - GFF3146 0.70 +0.1 25,228 - GFF3146 0.70 -0.3 25,258 - GFF3146 0.74 -0.2 25,258 - GFF3146 0.74 -0.0 25,477 + -1.1 25,478 - +0.8 25,478 - +0.4 25,657 + GFF3147 0.13 -0.3 25,658 - GFF3147 0.13 -0.1 25,660 + GFF3147 0.13 -0.7 25,661 - GFF3147 0.13 +0.2 25,661 - GFF3147 0.13 +0.6 25,768 + GFF3147 0.21 -0.5 26,916 + +0.1 26,916 + -0.5 26,917 - +1.1 26,917 - -0.3 26,917 - +0.3 27,337 - GFF3148 0.45 +0.1 27,337 - GFF3148 0.45 -0.2 27,402 + GFF3148 0.52 -0.9 27,402 + GFF3148 0.52 -0.7 27,403 - GFF3148 0.52 +0.9 27,403 - GFF3148 0.52 -0.1
Or see this region's nucleotide sequence