Experiment: Ying_all64 rep A; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2766 and GFF2767 are separated by 8 nucleotides GFF2767 and GFF2768 are separated by 95 nucleotides GFF2768 and GFF2769 are separated by 166 nucleotides
GFF2766 - Ribosomal-protein-S18p-alanine acetyltransferase (EC 2.3.1.-), at 365,504 to 366,085
GFF2766
GFF2767 - TsaB protein, required for threonylcarbamoyladenosine (t(6)A) formation in tRNA, at 366,094 to 366,852
GFF2767
GFF2768 - D-alanyl-D-alanine carboxypeptidase (EC 3.4.16.4), at 366,948 to 368,495
GFF2768
GFF2769 - TsaC protein (YrdC domain) required for threonylcarbamoyladenosine t(6)A37 modification in tRNA, at 368,662 to 369,678
GFF2769
Position (kb) 366
367
368
369 Strain fitness (log2 ratio) -1
0
1 at 367.230 kb on + strand, within GFF2768 at 367.231 kb on - strand, within GFF2768 at 367.231 kb on - strand, within GFF2768 at 367.282 kb on - strand, within GFF2768 at 367.282 kb on - strand, within GFF2768 at 367.282 kb on - strand, within GFF2768 at 367.326 kb on + strand, within GFF2768 at 367.326 kb on + strand, within GFF2768 at 367.326 kb on + strand, within GFF2768 at 367.326 kb on + strand, within GFF2768 at 367.326 kb on + strand, within GFF2768 at 367.327 kb on - strand, within GFF2768 at 367.327 kb on - strand, within GFF2768 at 367.380 kb on + strand, within GFF2768 at 367.381 kb on - strand, within GFF2768 at 367.381 kb on - strand, within GFF2768 at 367.381 kb on - strand, within GFF2768 at 367.381 kb on - strand, within GFF2768 at 367.500 kb on + strand, within GFF2768 at 367.500 kb on + strand, within GFF2768 at 367.501 kb on - strand, within GFF2768 at 367.501 kb on - strand, within GFF2768 at 367.782 kb on + strand, within GFF2768 at 367.782 kb on + strand, within GFF2768 at 367.782 kb on + strand, within GFF2768 at 368.076 kb on + strand, within GFF2768 at 368.400 kb on + strand at 368.400 kb on + strand at 368.400 kb on + strand at 368.401 kb on - strand at 368.401 kb on - strand at 368.498 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_all64 rep A; time point 2 remove 367,230 + GFF2768 0.18 +0.6 367,231 - GFF2768 0.18 -0.2 367,231 - GFF2768 0.18 +0.3 367,282 - GFF2768 0.22 -0.2 367,282 - GFF2768 0.22 -0.7 367,282 - GFF2768 0.22 -0.3 367,326 + GFF2768 0.24 +1.5 367,326 + GFF2768 0.24 +0.5 367,326 + GFF2768 0.24 -0.5 367,326 + GFF2768 0.24 -0.8 367,326 + GFF2768 0.24 +0.2 367,327 - GFF2768 0.24 +0.9 367,327 - GFF2768 0.24 -0.6 367,380 + GFF2768 0.28 -0.2 367,381 - GFF2768 0.28 -1.0 367,381 - GFF2768 0.28 +0.3 367,381 - GFF2768 0.28 +1.1 367,381 - GFF2768 0.28 +0.8 367,500 + GFF2768 0.36 +0.1 367,500 + GFF2768 0.36 +0.3 367,501 - GFF2768 0.36 -0.6 367,501 - GFF2768 0.36 +0.3 367,782 + GFF2768 0.54 +0.4 367,782 + GFF2768 0.54 -0.2 367,782 + GFF2768 0.54 +0.0 368,076 + GFF2768 0.73 -0.5 368,400 + -0.8 368,400 + +0.3 368,400 + -0.6 368,401 - -1.0 368,401 - +0.4 368,498 + +0.7
Or see this region's nucleotide sequence