Strain Fitness in Escherichia coli ECRC101 around MCAODC_28010

Experiment: TP9

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntmhpD and mhpC are separated by 9 nucleotidesmhpC and mhpB are separated by 17 nucleotidesmhpB and mhpA are separated by 1 nucleotides MCAODC_28000: mhpD - 2-keto-4-pentenoate hydratase, at 2,348,945 to 2,349,754 mhpD MCAODC_28005: mhpC - 2-hydroxy-6-oxonona-2,4-dienedioate hydrolase, at 2,349,764 to 2,350,630 mhpC MCAODC_28010: mhpB - 3-carboxyethylcatechol 2,3-dioxygenase, at 2,350,648 to 2,351,592 mhpB MCAODC_28015: mhpA - bifunctional 3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid hydroxylase, at 2,351,594 to 2,353,258 mhpA Position (kb) 2350 2351 2352Strain fitness (log2 ratio) -2 -1 0 1 2 3 4at 2349.742 kb on + strandat 2349.743 kb on - strandat 2349.765 kb on + strandat 2349.850 kb on + strandat 2349.850 kb on + strandat 2349.850 kb on + strandat 2349.851 kb on - strand, within mhpCat 2349.851 kb on - strand, within mhpCat 2350.010 kb on - strand, within mhpCat 2350.045 kb on + strand, within mhpCat 2350.045 kb on + strand, within mhpCat 2350.045 kb on + strand, within mhpCat 2350.046 kb on - strand, within mhpCat 2350.049 kb on - strand, within mhpCat 2350.090 kb on + strand, within mhpCat 2350.146 kb on + strand, within mhpCat 2350.165 kb on + strand, within mhpCat 2350.165 kb on + strand, within mhpCat 2350.166 kb on - strand, within mhpCat 2350.168 kb on + strand, within mhpCat 2350.169 kb on - strand, within mhpCat 2350.169 kb on - strand, within mhpCat 2350.189 kb on + strand, within mhpCat 2350.276 kb on + strand, within mhpCat 2350.277 kb on - strand, within mhpCat 2350.277 kb on - strand, within mhpCat 2350.277 kb on - strand, within mhpCat 2350.379 kb on - strand, within mhpCat 2350.460 kb on - strand, within mhpCat 2350.581 kb on - strandat 2350.623 kb on + strandat 2350.646 kb on - strandat 2350.669 kb on + strandat 2350.670 kb on - strandat 2350.713 kb on - strandat 2350.726 kb on - strandat 2350.843 kb on + strand, within mhpBat 2350.843 kb on + strand, within mhpBat 2350.844 kb on - strand, within mhpBat 2350.844 kb on - strand, within mhpBat 2351.077 kb on + strand, within mhpBat 2351.206 kb on - strand, within mhpBat 2351.262 kb on + strand, within mhpBat 2351.262 kb on + strand, within mhpBat 2351.262 kb on + strand, within mhpBat 2351.262 kb on + strand, within mhpBat 2351.262 kb on + strand, within mhpBat 2351.262 kb on + strand, within mhpBat 2351.263 kb on - strand, within mhpBat 2351.263 kb on - strand, within mhpBat 2351.263 kb on - strand, within mhpBat 2351.263 kb on - strand, within mhpBat 2351.263 kb on - strand, within mhpBat 2351.417 kb on + strand, within mhpBat 2351.433 kb on - strand, within mhpBat 2351.433 kb on - strand, within mhpBat 2351.433 kb on - strand, within mhpBat 2351.433 kb on - strand, within mhpBat 2351.482 kb on - strand, within mhpBat 2351.482 kb on - strand, within mhpBat 2351.547 kb on - strandat 2351.582 kb on + strandat 2351.582 kb on + strandat 2351.593 kb on - strandat 2351.593 kb on - strandat 2351.659 kb on + strandat 2351.759 kb on - strandat 2351.759 kb on - strandat 2351.789 kb on + strand, within mhpAat 2351.789 kb on + strand, within mhpAat 2351.789 kb on + strand, within mhpAat 2351.790 kb on - strand, within mhpAat 2351.790 kb on - strand, within mhpAat 2351.790 kb on - strand, within mhpAat 2351.792 kb on + strand, within mhpAat 2351.792 kb on + strand, within mhpAat 2351.793 kb on - strand, within mhpAat 2351.793 kb on - strand, within mhpAat 2351.927 kb on + strand, within mhpAat 2351.927 kb on + strand, within mhpAat 2351.927 kb on + strand, within mhpAat 2351.927 kb on + strand, within mhpAat 2352.064 kb on - strand, within mhpAat 2352.064 kb on - strand, within mhpAat 2352.099 kb on + strand, within mhpAat 2352.099 kb on + strand, within mhpAat 2352.099 kb on + strand, within mhpAat 2352.120 kb on + strand, within mhpAat 2352.149 kb on + strand, within mhpAat 2352.149 kb on + strand, within mhpAat 2352.149 kb on + strand, within mhpAat 2352.150 kb on - strand, within mhpAat 2352.150 kb on - strand, within mhpAat 2352.246 kb on + strand, within mhpAat 2352.246 kb on + strand, within mhpAat 2352.246 kb on + strand, within mhpAat 2352.246 kb on + strand, within mhpAat 2352.246 kb on + strand, within mhpAat 2352.246 kb on + strand, within mhpAat 2352.246 kb on + strand, within mhpAat 2352.247 kb on - strand, within mhpAat 2352.247 kb on - strand, within mhpAat 2352.247 kb on - strand, within mhpAat 2352.326 kb on - strand, within mhpAat 2352.334 kb on + strand, within mhpAat 2352.335 kb on - strand, within mhpAat 2352.335 kb on - strand, within mhpAat 2352.336 kb on + strand, within mhpAat 2352.336 kb on + strand, within mhpAat 2352.337 kb on - strand, within mhpAat 2352.337 kb on - strand, within mhpAat 2352.390 kb on - strand, within mhpAat 2352.436 kb on - strand, within mhpAat 2352.436 kb on - strand, within mhpAat 2352.551 kb on - strand, within mhpAat 2352.566 kb on + strand, within mhpAat 2352.566 kb on + strand, within mhpAat 2352.566 kb on + strand, within mhpAat 2352.566 kb on + strand, within mhpAat 2352.567 kb on - strand, within mhpA

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Per-strain Table

Position Strand Gene LocusTag Fraction TP9
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2,349,742 + -0.5
2,349,743 - -0.2
2,349,765 + +0.1
2,349,850 + +0.1
2,349,850 + +1.1
2,349,850 + +1.3
2,349,851 - mhpC MCAODC_28005 0.10 +0.2
2,349,851 - mhpC MCAODC_28005 0.10 -1.0
2,350,010 - mhpC MCAODC_28005 0.28 -0.5
2,350,045 + mhpC MCAODC_28005 0.32 +0.1
2,350,045 + mhpC MCAODC_28005 0.32 -0.1
2,350,045 + mhpC MCAODC_28005 0.32 -0.9
2,350,046 - mhpC MCAODC_28005 0.33 -0.6
2,350,049 - mhpC MCAODC_28005 0.33 -0.0
2,350,090 + mhpC MCAODC_28005 0.38 +0.9
2,350,146 + mhpC MCAODC_28005 0.44 +0.0
2,350,165 + mhpC MCAODC_28005 0.46 -1.0
2,350,165 + mhpC MCAODC_28005 0.46 +0.5
2,350,166 - mhpC MCAODC_28005 0.46 -0.3
2,350,168 + mhpC MCAODC_28005 0.47 +1.7
2,350,169 - mhpC MCAODC_28005 0.47 +1.7
2,350,169 - mhpC MCAODC_28005 0.47 -1.0
2,350,189 + mhpC MCAODC_28005 0.49 -0.6
2,350,276 + mhpC MCAODC_28005 0.59 +0.1
2,350,277 - mhpC MCAODC_28005 0.59 +2.3
2,350,277 - mhpC MCAODC_28005 0.59 -1.1
2,350,277 - mhpC MCAODC_28005 0.59 -1.0
2,350,379 - mhpC MCAODC_28005 0.71 +0.5
2,350,460 - mhpC MCAODC_28005 0.80 -0.1
2,350,581 - +0.7
2,350,623 + -1.1
2,350,646 - +0.0
2,350,669 + -0.3
2,350,670 - -0.5
2,350,713 - +0.2
2,350,726 - +0.7
2,350,843 + mhpB MCAODC_28010 0.21 -1.9
2,350,843 + mhpB MCAODC_28010 0.21 +1.5
2,350,844 - mhpB MCAODC_28010 0.21 -0.9
2,350,844 - mhpB MCAODC_28010 0.21 -0.9
2,351,077 + mhpB MCAODC_28010 0.45 -0.8
2,351,206 - mhpB MCAODC_28010 0.59 +0.2
2,351,262 + mhpB MCAODC_28010 0.65 -1.0
2,351,262 + mhpB MCAODC_28010 0.65 -0.5
2,351,262 + mhpB MCAODC_28010 0.65 -1.3
2,351,262 + mhpB MCAODC_28010 0.65 -0.9
2,351,262 + mhpB MCAODC_28010 0.65 -0.4
2,351,262 + mhpB MCAODC_28010 0.65 -1.6
2,351,263 - mhpB MCAODC_28010 0.65 +0.3
2,351,263 - mhpB MCAODC_28010 0.65 -1.0
2,351,263 - mhpB MCAODC_28010 0.65 +0.1
2,351,263 - mhpB MCAODC_28010 0.65 -1.0
2,351,263 - mhpB MCAODC_28010 0.65 -1.0
2,351,417 + mhpB MCAODC_28010 0.81 -0.9
2,351,433 - mhpB MCAODC_28010 0.83 +0.7
2,351,433 - mhpB MCAODC_28010 0.83 -0.8
2,351,433 - mhpB MCAODC_28010 0.83 +1.0
2,351,433 - mhpB MCAODC_28010 0.83 -1.7
2,351,482 - mhpB MCAODC_28010 0.88 -0.3
2,351,482 - mhpB MCAODC_28010 0.88 -0.6
2,351,547 - -0.3
2,351,582 + +0.6
2,351,582 + +0.2
2,351,593 - -1.2
2,351,593 - -1.3
2,351,659 + +0.0
2,351,759 - -0.8
2,351,759 - -0.5
2,351,789 + mhpA MCAODC_28015 0.12 -1.4
2,351,789 + mhpA MCAODC_28015 0.12 -0.6
2,351,789 + mhpA MCAODC_28015 0.12 -0.9
2,351,790 - mhpA MCAODC_28015 0.12 -0.5
2,351,790 - mhpA MCAODC_28015 0.12 +1.1
2,351,790 - mhpA MCAODC_28015 0.12 +1.1
2,351,792 + mhpA MCAODC_28015 0.12 +0.2
2,351,792 + mhpA MCAODC_28015 0.12 -0.8
2,351,793 - mhpA MCAODC_28015 0.12 +0.3
2,351,793 - mhpA MCAODC_28015 0.12 -0.1
2,351,927 + mhpA MCAODC_28015 0.20 +0.1
2,351,927 + mhpA MCAODC_28015 0.20 -0.7
2,351,927 + mhpA MCAODC_28015 0.20 -0.1
2,351,927 + mhpA MCAODC_28015 0.20 +1.7
2,352,064 - mhpA MCAODC_28015 0.28 +1.2
2,352,064 - mhpA MCAODC_28015 0.28 -1.9
2,352,099 + mhpA MCAODC_28015 0.30 -0.3
2,352,099 + mhpA MCAODC_28015 0.30 -0.2
2,352,099 + mhpA MCAODC_28015 0.30 +0.4
2,352,120 + mhpA MCAODC_28015 0.32 -0.4
2,352,149 + mhpA MCAODC_28015 0.33 -1.9
2,352,149 + mhpA MCAODC_28015 0.33 -0.6
2,352,149 + mhpA MCAODC_28015 0.33 +0.0
2,352,150 - mhpA MCAODC_28015 0.33 -0.9
2,352,150 - mhpA MCAODC_28015 0.33 +0.1
2,352,246 + mhpA MCAODC_28015 0.39 -0.1
2,352,246 + mhpA MCAODC_28015 0.39 +0.1
2,352,246 + mhpA MCAODC_28015 0.39 +1.1
2,352,246 + mhpA MCAODC_28015 0.39 +0.3
2,352,246 + mhpA MCAODC_28015 0.39 -0.5
2,352,246 + mhpA MCAODC_28015 0.39 +0.4
2,352,246 + mhpA MCAODC_28015 0.39 -0.3
2,352,247 - mhpA MCAODC_28015 0.39 -0.3
2,352,247 - mhpA MCAODC_28015 0.39 -0.7
2,352,247 - mhpA MCAODC_28015 0.39 +1.3
2,352,326 - mhpA MCAODC_28015 0.44 -1.3
2,352,334 + mhpA MCAODC_28015 0.44 +0.6
2,352,335 - mhpA MCAODC_28015 0.45 -0.4
2,352,335 - mhpA MCAODC_28015 0.45 -1.0
2,352,336 + mhpA MCAODC_28015 0.45 -0.3
2,352,336 + mhpA MCAODC_28015 0.45 -0.7
2,352,337 - mhpA MCAODC_28015 0.45 -1.6
2,352,337 - mhpA MCAODC_28015 0.45 -1.0
2,352,390 - mhpA MCAODC_28015 0.48 -0.1
2,352,436 - mhpA MCAODC_28015 0.51 +0.3
2,352,436 - mhpA MCAODC_28015 0.51 -2.2
2,352,551 - mhpA MCAODC_28015 0.57 -0.9
2,352,566 + mhpA MCAODC_28015 0.58 +4.1
2,352,566 + mhpA MCAODC_28015 0.58 +1.7
2,352,566 + mhpA MCAODC_28015 0.58 +1.5
2,352,566 + mhpA MCAODC_28015 0.58 +1.6
2,352,567 - mhpA MCAODC_28015 0.58 +0.4

Or see this region's nucleotide sequence