Strain Fitness in Escherichia coli ECRC101 around MCAODC_22000

Experiment: TP9

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntmenH and MCAODC_22000 are separated by 302 nucleotidesMCAODC_22000 and tnpB are separated by 49 nucleotidestnpB and insE overlap by 4 nucleotidesinsE and tfaX are separated by 75 nucleotides MCAODC_21995: menH - alpha/beta hydrolase, at 1,179,690 to 1,181,174 menH MCAODC_22000: MCAODC_22000 - IS66-like element ISEc8 family transposase, at 1,181,477 to 1,183,015 _22000 MCAODC_22005: tnpB - IS66 family insertion sequence element accessory protein TnpB, at 1,183,065 to 1,183,412 tnpB MCAODC_22010: insE - Transposase InsE and inactivated derivatives, at 1,183,409 to 1,183,789 insE MCAODC_22015: tfaX - Protein TfaX, at 1,183,865 to 1,184,113 tfaX Position (kb) 1181 1182 1183 1184Strain fitness (log2 ratio) -1 0 1 2at 1180.503 kb on - strand, within menHat 1180.528 kb on + strand, within menHat 1180.528 kb on + strand, within menHat 1180.529 kb on - strand, within menHat 1180.558 kb on + strand, within menHat 1180.885 kb on + strand, within menHat 1180.948 kb on + strand, within menHat 1181.030 kb on - strandat 1181.032 kb on + strandat 1181.135 kb on + strandat 1181.216 kb on - strandat 1181.403 kb on - strandat 1181.437 kb on - strandat 1181.448 kb on - strandat 1183.887 kb on - strandat 1183.895 kb on - strand, within tfaXat 1183.895 kb on - strand, within tfaXat 1183.976 kb on + strand, within tfaXat 1183.977 kb on - strand, within tfaXat 1184.000 kb on - strand, within tfaXat 1184.002 kb on - strand, within tfaXat 1184.002 kb on - strand, within tfaX

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Per-strain Table

Position Strand Gene LocusTag Fraction TP9
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1,180,503 - menH MCAODC_21995 0.55 +2.5
1,180,528 + menH MCAODC_21995 0.56 +1.5
1,180,528 + menH MCAODC_21995 0.56 -0.6
1,180,529 - menH MCAODC_21995 0.56 -0.3
1,180,558 + menH MCAODC_21995 0.58 -0.2
1,180,885 + menH MCAODC_21995 0.80 -0.2
1,180,948 + menH MCAODC_21995 0.85 -1.4
1,181,030 - +1.0
1,181,032 + +0.8
1,181,135 + -0.6
1,181,216 - -0.4
1,181,403 - +0.2
1,181,437 - -0.0
1,181,448 - +0.4
1,183,887 - +0.2
1,183,895 - tfaX MCAODC_22015 0.12 -0.2
1,183,895 - tfaX MCAODC_22015 0.12 +0.8
1,183,976 + tfaX MCAODC_22015 0.45 +0.6
1,183,977 - tfaX MCAODC_22015 0.45 -1.4
1,184,000 - tfaX MCAODC_22015 0.54 +0.3
1,184,002 - tfaX MCAODC_22015 0.55 -0.4
1,184,002 - tfaX MCAODC_22015 0.55 -0.8

Or see this region's nucleotide sequence